Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study

Зиганшина, М. М.; Долгушина, Н. В.; Kulikova, G. V.; Fayzullina, N.M.; Yarotskaya, Ekaterina; Khasbiullina, Nailia R.; Abdurakhmanova, Nigora F.; Asaturova, Asaturova A.V.; Щеголев, А. И.; Dovgan, Alina A.; Сухих, Г. Т.

doi:10.1186/s12958-021-00750-z

Cited by 9 publications

(7 citation statements)

References 53 publications

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“…In this review, we focused on glycan recognition by peripheral NK cells and modulation of activation toward the cytotoxic phenotype to uncover new insights into molecular communication mechanisms between the trophoblast and the peripheral innate immune system. In the last two decades, a glycobiological perspective on pregnancy has emerged ( 32 – 35 ), and these new approaches have the potential to provide critical insights into the pathogenesis of preeclampsia and the development of severe clinical forms of the disease ( 36 – 43 ).…”

Section: Introductionmentioning

confidence: 99%

Expression of placental glycans and its role in regulating peripheral blood NK cells during preeclampsia: a perspective

et al. 2023

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Preeclampsia is a pregnancy-related multisystem disorder characterized by altered trophoblast invasion, oxidative stress, exacerbation of systemic inflammatory response, and endothelial damage. The pathogenesis includes hypertension and mild-to-severe microangiopathy in the kidney, liver, placenta, and brain. The main mechanisms involved in its pathogenesis have been proposed to limit trophoblast invasion and increase the release of extracellular vesicles from the syncytiotrophoblast into the maternal circulation, exacerbating the systemic inflammatory response. The placenta expresses glycans as part of its development and maternal immune tolerance during gestation. The expression profile of glycans at the maternal–fetal interface may play a fundamental role in physiological pregnancy changes and disorders such as preeclampsia. It is unclear whether glycans and their lectin-like receptors are involved in the mechanisms of maternal–fetal recognition by immune cells during pregnancy homeostasis. The expression profile of glycans appears to be altered in hypertensive disorders of pregnancy, which could lead to alterations in the placental microenvironment and vascular endothelium in pregnancy conditions such as preeclampsia. Glycans with immunomodulatory properties at the maternal–fetal interface are altered in early-onset severe preeclampsia, implying that innate immune system components, such as NK cells, exacerbate the systemic inflammatory response observed in preeclampsia. In this article, we discuss the evidence for the role of glycans in gestational physiology and the perspective of glycobiology on the pathophysiology of hypertensive disorders in gestation.

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Section: Introductionmentioning

confidence: 99%

Expression of placental glycans and its role in regulating peripheral blood NK cells during preeclampsia: a perspective

et al. 2023

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“…Over the years, a number of hypotheses have been proposed regarding the pathogenesis of thin endometrium, including increased cellular senescence, excessive collagen deposition and downregulation of gene expression, based on thin uterine disease models ( Ziganshina et al, 2021 ; Lv et al, 2022 ). Therefore, a variety of therapeutically suggested therapies have been created to target these pathogenic pathways.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor 1 ameliorates thin endometrium in rats through activation of the autophagic pathway

Zhu

Yin

et al. 2023

Front. Pharmacol.

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Background: Thin endometrium is a reproductive disorder that affects embryo implantation. There are several therapies available for this disease, however they are not so effective. Fibroblast growth factor 1 (FGF1) is a member of fibroblast growth factor superfamily (FGFs), and it has been demonstrated that FGF1 expression was altered in samples collected from patients with thin endometrium. However, it is unclear if FGF1 could improve thin endometrium. The aim of this study was to investigate whether FGF1 have a therapeutic effect on thin endometrium.Methods: A model of thin endometrium induced by ethanol was constructed to investigate the effect and mechanism of action of FGF1 in thin endometrium. In the characterization experiments, 6–8 weeks female rats (n = 40) were divided into four groups: i) Control group; ii) Sham group; iii) Injured group; (iv) FGF1 therapy group. Endometrial tissues would be removed after three sexuel cycles after molding. Morphology and histology of the endometrium were evaluated by visual and hematoxylin and eosin staining. Masson staining and expression of α-SMA in endometrium showed the degree of endometrial fibrosis. Western blotting (PCNA、vWF and Vim) and immunohistochemistry (CK19 and MUC-1) demonstrated the effect of FGF1 on cell proliferation and angiogenesis. Moreover, immunohistochemistry (ER and PR) was used to explore the function of endometrium. The remaining rats (n = 36) were divided into three groups: i) Injured group; ii) FGF1 therapy group; and iii) 3-methyladenine. Western blotting (p38、p-p38、PI3K 、SQSTM1/p62、beclin-1 and LC3) was used to explore the mechanisms of FGF1.Results: In FGF1 therapy group, the morphology and histology of endometrium improved compared with the model group. Masson staining and the expression level of α-SMA showed that FGF1 could decrease the fibrotic area of endometrium. Besides, changes in ER and PR expression in the endometrium suggested that FGF1 could restore endometrium-related functions. Western blotting and immunohistochemistry revealed that PCNA, vWF, Vim, CK19 and MUC-1 were significantly increased after FGF1 treatment compared with the thin endometrium. In addition, Western blotting showed that p38, p-p38, PI3K, SQSTM1/p62, beclin-1 and LC3 levels were higher in FGF1 group than in the injured group.Conclusion: FGF1 application cured the thin endometrium caused by ethanol through autophagy mechanism.

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“…An example of such an environmental modulation is that in certain hostile conditions that alpacas are exposed, these animals mobilize their own micronutrients like selenium to support the survival of the conceptus. This transplacental nutrient transportation is highly influenced by the glycosylation pattern, once a modification in this profile may compromise the maternal–fetal communication ( Ziganshina et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

Silva

et al. 2023

Front. Cell Dev. Biol.

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Alpaca is a South American camelid, particularly present in Peruvian highlands, where oxygen concentration and atmospheric pressure are very low. Due to this fact, gestational physiology has adapted to preserve the conceptus’ and mother’s health. In this context, several cellular and molecular features play an essential role during and at the end of gestation. Structural carbohydrates act on maternal–fetal communication, recognize exogenous molecules, and contribute to placental barrier selectivity. Therefore, this study aimed to characterize the structural carbohydrate profiles that are present in the term alpaca placenta, kept in their natural habitat of around 4,000 m height. For this propose, 12 term alpaca placentas were collected, and the material was obtained at the time of birth from camelids raised naturally in the Peruvian highlands, in the Cusco region. All placenta samples were processed for histological analysis. A lectin histochemical investigation was performed using 13 biotinylated lectins, allowing us to determine the location of carbohydrates and their intensity on a semi-quantitative scale. Our results demonstrated that during term gestation, the epitheliochorial alpaca placenta shows a high presence of carbohydrates, particularly glucose, α-linked mannose, N-acetylglucosamine β (GlcNAc), galactose (αGal), and N-acetylgalactosamine α (GalNAc), present in the trophoblast, amnion epithelium, and mesenchyme, as well as the presence of sialic acid residues and low affinity for fucose. In fetal blood capillaries, the presence of bi- and tri-antennary complex structures and α-linked mannose was predominated. In conclusion, we characterized the glycosylation profile in the term alpaca placenta. Based on our data, compared to those reported in the bibliography, we suggest that these carbohydrates could participate in the labor of these animals that survive in Peruvian extreme environments.

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Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study

Cited by 9 publications

References 53 publications

Expression of placental glycans and its role in regulating peripheral blood NK cells during preeclampsia: a perspective

Expression of placental glycans and its role in regulating peripheral blood NK cells during preeclampsia: a perspective

Fibroblast growth factor 1 ameliorates thin endometrium in rats through activation of the autophagic pathway

Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

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