Epithelial and stromal uterine cells cultured in vitro protect bovine sperm from hydrogen peroxide

Lapointe, Serge; Bilodeau, Jean‐François; D, Lemieux; Asselin, Éric; Ma, Fortier; Sirard, Marc‐André

doi:10.1016/s0093-691x(00)00354-x

Cited by 15 publications

(5 citation statements)

References 57 publications

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“…Those effects were also observed in the human model [101, 105]. However, the same response is observed when spermatozoa are coincubated with cells from different tissue origin, including oviductal epithelial cells [106, 107]. Studies carried out in bovine demonstrated that several protease inhibitors [108] and a 42-kDa protein are secreted and are capable of maintaining the sperm motility.…”

Section: The Role Of Epididymis In Sperm Survival and The Acquisitmentioning

confidence: 79%

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Caballero

Frenette

Sullivan

2011

Veterinary Medicine International

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After spermatogenesis, testicular spermatozoa are not able to fertilize an oocyte, they must undergo sequential maturational processes. Part of these essential processes occurs during the transit of the spermatozoa through the male reproductive tract. Since the sperm become silent in terms of translation and transcription at the testicular level, all the maturational changes that take place on them are dependent on the interaction of spermatozoa with epididymal and accessory gland fluids. During the last decades, reproductive biotechnologies applied to bovine species have advanced significantly. The knowledge of the bull reproductive physiology is really important for the improvement of these techniques and the development of new ones. This paper focuses on the importance of the sperm interaction with the male reproductive fluids to acquire the fertilizing ability, with special attention to the role of the membranous vesicles present in those fluids and the recent mechanisms of protein acquisition during sperm maturation.

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Section: The Role Of Epididymis In Sperm Survival and The Acquisitmentioning

confidence: 79%

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Caballero

Frenette

Sullivan

2011

Veterinary Medicine International

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“…The effects of ROS are prevented or diminished by the detoxifying enzymes superoxide dismutase (SOD), catalase, peroxidases (GPx and PHGPx), and by reducing agents, such as glutathione, ascorbic acid, taurine and hypotaurine, present in the sperm cell and sperm environment (Hinton et al. 1995, Lapoint et al. 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Although its toxic effects are unknown, ROS can be formed after peroxidation and produce citotoxic substances (Pires de Melo et al 1997). The effects of ROS are prevented or diminished by the detoxifying enzymes superoxide dismutase (SOD), catalase, peroxidases (GPx and PHGPx), and by reducing agents, such as glutathione, ascorbic acid, taurine and hypotaurine, present in the sperm cell and sperm environment (Hinton et al 1995, Lapoint et al 2000. Silva et al (2006) using the same freezing protocol with a Tris extender obtained similar results of intact plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Fertilizing Potential of Frozen‐thawed dog Spermatozoa Diluted in ACP‐106^®using anIn VitroSperm–Oocyte Interaction Assay

Cardoso

Silva

et al. 2007

Reprod Domestic Animals

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The aim of present study was to evaluate frozen canine semen with ACP-106 (Powder Coconut Water) using an in vitro sperm--oocyte interaction assay (SOIA). Ten ejaculates from five stud dogs were diluted in ACP-106 containing 20% egg yolk, submitted to cooling in a thermal box for 40 min and in a refrigerator for 30 min. After this period, a second dilution was performed using ACP-106 containing 20% egg yolk and 12% glycerol. Samples were thawed at 38 degrees C for 1 min. Post-thaw motility was evaluated by light microscopy and by using a computer aided semen analysis (CASA). Plasma membrane integrity and sperm morphology/acrosomal status were evaluated by fluorescent probes (C-FDA/PI) and Bengal Rose respectively. Moreover, frozen-thawed semen was analysed by a SOIA. Subjective post-thaw motility was 52.0 +/- 14.8% and it was significant higher than the total motility estimated by CASA (23.0 +/- 14.8%) because this system considered the egg yolk debris as immotile spermatozoa. Although normal sperm rate and acrosomal integrity evaluated by Bengal Rose stain was 89.6 +/- 3.1% and 94.3 +/- 3.1%, respectively, post-thaw percentage of intact plasma membrane was only 35.1 +/- 14.3%. Regarding SOIA, the percentage of interacted oocytes (bound, penetrated and bound and/or penetrated) was 75.3%. Using regression analysis, it was found significant relations between some CASA patterns and data for SOIA. In conclusion, the freezing-thawing procedure using ACP-106 was efficient for maintain the in vitro fertility potential of dog spermatozoa.

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“…Several in vitro studies have shown that bovine sperm motility can be efficiently maintained by coincubation with cultured cells from different tissue origin (Ashizawa et al, 1982;Ijaz et al, 1994;Lapointe et al, 2000), including bovine epididymal epithelial cells Reyes-Moreno et al, 2000). Epididymal cell culture systems were also developed for rodent (Moore and Hartman, 1986;Smith et al, 1986;Byers et al, 1992;Bongso and Trounson, 1996), and human (Cooper et al, 1990;Moore et al, 1992Moore et al, , 1998.…”

Section: Discussionmentioning

confidence: 99%

Characterization of secretory proteins from cultured cauda epididymal cells that significantly sustain bovine sperm motility in vitro

Reyes‐Moreno

Boilard

Sullivan

et al. 2002

Molecular Reproduction Devel

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Epididymis provides a safe environment in which stored-spermatozoa could survive for days before ejaculation. In vitro studies suggested that epididymal proteins seem to be implicated in sperm survival during coincubation with cultured epididymal cells. This study was basically designed to confirm if secretory proteins from bovine epididymal cell cultures provide sperm protection against rapid loss of sperm motility in vitro. Bovine spermatozoa were incubated in conditioned media (CM), which were prepared from cultured cauda epididymal cell (CEC). Motion parameters were recorded using a computer-assisted sperm analyzer. Sperm-free protein extracts from CM were fractionated by ultrafiltration through a 10-kDa cut off membrane. A significantly positive effect on sperm motility was observed when spermatozoa were incubated in CM (54 +/- 4%) and CM > 10 kDa (57 +/- 4%) compared to CM < 10-kDa fraction (30 +/- 3%) or fresh media (34 +/- 3%), after a 6-hr incubation period. This beneficial effect on sperm motility was abolished when the CM > 10-kDa fraction was heat-treated at 100 degrees C for 10 min. The CM > 10 kDa fraction provides factors that remained active even though spermatozoa were washed twice after a 2-hr preincubation period. To identify potential beneficial factors, bovine spermatozoa were incubated with radiolabeled proteins obtained using (35)S-methionine in culture medium. SDS-PAGE analysis of proteins extracted from CM-preincubated spermatozoa revealed the presence of a 42-kDa protein strongly associated to the sperm surface. This 42-kDa spot was trypsin-digested and identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) as a protein homologue to a 35-kDa bovine estrogen-sulfotransferase. This protein can play a role in epididymal biology and sperm function. Taken together, these results suggest that specific epididymal proteins can be implicated in the sperm protection in vitro, and can be characterized in our cell culture system.

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Epithelial and stromal uterine cells cultured in vitro protect bovine sperm from hydrogen peroxide

Cited by 15 publications

References 57 publications

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Evaluation of Fertilizing Potential of Frozen‐thawed dog Spermatozoa Diluted in ACP‐106^®using anIn VitroSperm–Oocyte Interaction Assay

Characterization of secretory proteins from cultured cauda epididymal cells that significantly sustain bovine sperm motility in vitro

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Epithelial and stromal uterine cells cultured in vitro protect bovine sperm from hydrogen peroxide

Cited by 15 publications

References 57 publications

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Evaluation of Fertilizing Potential of Frozen‐thawed dog Spermatozoa Diluted in ACP‐106®using anIn VitroSperm–Oocyte Interaction Assay

Characterization of secretory proteins from cultured cauda epididymal cells that significantly sustain bovine sperm motility in vitro

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Evaluation of Fertilizing Potential of Frozen‐thawed dog Spermatozoa Diluted in ACP‐106^®using anIn VitroSperm–Oocyte Interaction Assay