Episome-carried Surface Antigen K88 of<i>Escherichia coli</i>III. Morphology

Stirm, Stephan; Ørskov, Frits; Ørskov, Ida

doi:10.1128/jb.93.2.740-748.1967

Cited by 122 publications

(61 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Filters were washed and reacted with hydrogen peroxide substrate and 4chloronaphthol chromogen. The immunological detection of CS31A was controlled by SDS-PAGE analysis of extracts obtained by heating the bacterial suspensions at 60°C for 20 min [10]. After silver staining of the gel, the 29K CS31A fimbrial subunit was identified [5].…”

Section: Detection Of Firnbrial Antigens F165 and Cs31amentioning

confidence: 99%

Prevalence of CS31A and F165 surface antigens in Escherichia coli isolates from animals in France, Canada and India

Contrepois¹

1989

FEMS Microbiology Letters

View full text Add to dashboard Cite

Bovine and porcine enterotoxigenic and non-enterotoxigenic Escherichia coli isolates from France, Canada, and India were characterized with respect to serogroup and production of fimbrial antigens CS31A and F165. Of 231 bovine isolates from the 3 countries, 20.5% produced CS31A alone, 17.7% produced F165 alone, and 17.3% produced both CS31A and F165. On the other hand, of 84 porcine isolates from Canada, 1.2% produced CS31A alone, 14.3% produced F165 alone, and no isolate produced both CS31A and F165. CS31A was found together with F5 (K99) in 7 of 16 bovine enterotoxigenic E. coli isolates of serogroups 08, 09, 020, and 023, but was not found in any of 20 F4 (K88)- or 5 F6 (987P)-positive porcine enterotoxigenic E. coli isolates. F165 was not found in enterotoxigenic E. coli. Among non-enterotoxigenic isolates, CS31A and F165 were mainly associated with serogroups 08, 09, 011, 015, 017, 023, 025, 078, 0101, 0115, 0117, 0141, and 0153.

show abstract

Section: Detection Of Firnbrial Antigens F165 and Cs31amentioning

confidence: 99%

Prevalence of CS31A and F165 surface antigens in Escherichia coli isolates from animals in France, Canada and India

Contrepois¹

1989

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…This attribute is recognized as a fundamental feature of the bacterial intestinal pathogens that infect and cause acute diarrhoea in piglets, calves, lambs and humans (Gaastra & deGraff 1982). The attachment of the pathogen to the mucosa of the small intestine in these species is mediated by antigenically distinct bacterial surface adhesions (Brinton 196.5;Stirm et al 1967;Jones & Rutter 1972;Smith & Linggood 1972;0rskov etal. 197.5;Evans & Evans 1978;Isaacson et al 1978;Morris et al 1982), morphologically identified as fimbriae or pili (Duguid & Anderson 1967).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism and inheritance of swine small intestinal receptors mediating adhesion of three serological variants of Escherichia coli‐producing K88 pilus antigen

Rapacz

Hasler‐Rapacz

1986

Animal Genetics

View full text Add to dashboard Cite

Brush borders, enterocytes, or both preparations obtained from the small intestine of 345 pedigreed pigs, carrying components of seven breeds, were tested by adhesion assay in vitro with 6 3 2 enteropathogenic Escherichia coli strains, each expressing one of the three K88 pilus antigens, K88ab, K88ac and K88ad. With few exceptions, all pigs were classified as belonging to one of four adhesion phenotypes:

show abstract

“…The colonization factor antigens of E. coli resemble the type 1 pili morphologically, but cause mannose-resistant agglutination ofhuman and bovine erythrocytes (11,12). K88 and K99 are pilus-like antigens on the E. coli strains enteropathogenic for pig, calf, and lamb (17,36,37). The significance of the pili for bacterial survival and pathogenicity in vivo is uncertain.…”

mentioning

confidence: 99%

New Method for isolation of immunologically pure pili from Escherichia coli

Korhonen¹,

Nurmiaho²,

Ranta³

et al. 1980

Infect Immun

164

View full text Add to dashboard Cite

A new technique for purification of bacterial pili was developed and applied to Escherichia coli strains isolated from the urine of patients with symptomatic urinary tract infections. After mechanical detachment from the bacterial cells, the pili were concentrated by precipitation with ammonium sulfate, dialyzed, and solubilized in buffer containing deoxycholate. The fraction containing the pili was purified further by ultracentrifugation in a sucrose gradient and by elution through a Sepharose 4B column in 6 M urea buffer. The pilus filaments were not dissociated by concentrated urea and were eluted in the void volume of the column. The purified pili had a molecular weight of 17,000. The isoelectric point of the pili from one of the strains was 4.9, and about 43% of the amino acids were hydrophobic. Hyperimmunesera raised in rabbits against the purified pili did not contain detectable antibodies to the lipopolysaccharide 0 antigen or to the capsular polysaccharide K antigen of the homologous strain. The pili obtained by this purification procedure are free from other detectable bacterial surface antigens, and the purified pilus filaments are of relatively homogeneous size. This procedure enables purification of the pili also from flagellated strains.flasks. The medium volume was 'Ao of the volume of 569 on July 9, 2020 by guest http://iai.asm.org/ Downloaded from LITERATURE CITED 1. Blakesley, R. W., and J. A. Boezi. 1l77. A new staining on July 9, 2020 by guest http://iai.asm.org/ Downloaded from turforsch. 76:148-155.

show abstract

Episome-carried Surface Antigen K88 ofEscherichia coliIII. Morphology

Cited by 122 publications

References 18 publications

Prevalence of CS31A and F165 surface antigens in Escherichia coli isolates from animals in France, Canada and India

Prevalence of CS31A and F165 surface antigens in Escherichia coli isolates from animals in France, Canada and India

Polymorphism and inheritance of swine small intestinal receptors mediating adhesion of three serological variants of Escherichia coli‐producing K88 pilus antigen

New Method for isolation of immunologically pure pili from Escherichia coli

Contact Info

Product

Resources

About