2000
DOI: 10.1046/j.1432-1327.2000.01645.x
|View full text |Cite
|
Sign up to set email alerts
|

Episomal vectors for gene expression in mammalian cells

Abstract: An important reason for preferring mammalian cells for heterologous gene expression is their ability to make authentic proteins containing post-translational modifications similar to those of the native protein. The development of expression systems for mammalian cells has been ongoing for several years, resulting in a wide variety of effective expression vectors. The aim of this review is to highlight episomal expression vectors. Such episomal plasmids are usually based on sequences from DNA viruses, such as … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
102
1

Year Published

2005
2005
2023
2023

Publication Types

Select...
6
4

Relationship

0
10

Authors

Journals

citations
Cited by 129 publications
(104 citation statements)
references
References 188 publications
(256 reference statements)
0
102
1
Order By: Relevance
“…It shows that moss is capable of faithful episomal replication of foreign DNA without rearrangements. This is unlike episomal vectors in mammalian cells, where rearrangements and insertions of host DNA are frequent (19), and instead resembles yeast shuttle vectors. It does not rule out that replication in moss involves complex intermediates, such as episomal concatemers (15), since the latter could pop out circles by recombination (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…It shows that moss is capable of faithful episomal replication of foreign DNA without rearrangements. This is unlike episomal vectors in mammalian cells, where rearrangements and insertions of host DNA are frequent (19), and instead resembles yeast shuttle vectors. It does not rule out that replication in moss involves complex intermediates, such as episomal concatemers (15), since the latter could pop out circles by recombination (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…Cloning and Expression of HtrA1 Recombinant Proteins-Recombinant murine HtrA proteins were produced using a mammalian episomal expression vector (pCEP-His) and 293-EBNA cells (29). pCEP-His has been modified from the pCEP-pu/AC7 vector (30) to incorporate an N-terminal His 6 tag following a signal peptide of the BM40 protein (31).…”
Section: Methodsmentioning
confidence: 99%
“…The generation of artificial chromosomes with an optimal size with respect to stability as well as transfection efficiencies, can be promising for the future of nonviral gene therapy as it may form the perfect alternative for viral episomal gene vectors (see next paragraph). This is even more interesting in the context of possible immunogenic or oncogenic properties of viral vectors [171]. Additionally, the transgenes bear strong constitutive promoters, which result in nonphysiological overexpression.…”
Section: Nonviral Chromatin Tethering Factorsmentioning
confidence: 99%