Episomal and integrated human papillomavirus type 16 loads and anal intraepithelial neoplasia in HIV-seropositive men

Alvarez, Jennifer; Pokomandy, Alexandra de; Rouleau, Danielle; Ghattas, George; Vézina, Sylvie; Côté, Pierre; Allaire, Guy; Hadjeres, Rachid; Franco, Eduardo L.; Coutlée, François

doi:10.1097/qad.0b013e32833db9ea

Cited by 24 publications

(18 citation statements)

References 47 publications

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“…Also, we did not find an association with nadir CD4+ cell count. Some previous studies showed a significant correlation between a low nadir CD4 count and AIN [12,15,20], whereas other studies did not [11,16,19]. …”

Section: Discussionmentioning

confidence: 99%

“…The first study showed an OR of 0.18 for receiving cART[17], but in the latter study it was found that in only 4% of patients high grade lesions regressed after initiating cART [14]. Other studies do not show any beneficial effect of ART on AIN [11,12,15,16,19]. Likewise, contradictory results are reported on the influence of cART on CIN in HIV positive women [26-28].…”

Section: Discussionmentioning

confidence: 99%

“…The outcomes on other risk factors were conflicting. Two studies performed HRA as primary screening method [19,20]. The presence of high-risk HPV types, in particular HPV16, was a significant risk factor [20].…”

Section: Introductionmentioning

confidence: 99%

“…However, testing for the presence of high-risk HPV types is expensive and not generally available. In addition, these studies did not evaluate other potential risk factors like sexually transmitted infections (STI), anal enemas, sexual habits and drug use, and one of the two only included patients who were HPV 16 positive [19]. …”

Section: Introductionmentioning

confidence: 99%

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Risk Factors for the Presence of Anal Intraepithelial Neoplasia in HIV+ Men Who Have Sex with Men

et al. 2013

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ObjectiveAnal Intraepithelial Neoplasia (AIN) is present in the majority of HIV+ men who have sex with men (MSM) and routine AIN-screening is subject of discussion. In this study we analysed a wide range of potential risk factors for AIN in order to target screening programs. MethodsWe screened 311 HIV+ MSM by high resolution anoscopy, with biopsies of suspect lesions. HIV-parameters, previous sexual transmitted infections (STI’s), anal pathology, sexual practices and substance use were analysed in relation to AIN by uni- and multivariable logistic regression.ResultsAIN (any grade) was found in 175/311 MSM (56%), high grade (HG)AIN in 30%. In the univariable analysis, years since HIV diagnosis, years of antiretroviral therapy (cART) and anal XTC use decreased AIN risk, while a history of anogenital warts and use of GHB (γ-hydroxybutyric acid) increased this risk. In the multivariable analysis three parameters remained significant: years of cART (OR=0.92 per year, p=0.003), anal XTC use (OR=0.10, p=0.002) and GHB use (OR=2.60, p=0.003). No parameters were significantly associated with HGAIN, but there was a trend towards increased risk with anal enema use prior to sex (>50 times ever; p=0.07) and with a history of AIN (p=0.06). CD4 count, STI’s, anal pathology, smoking, number of sex partners and anal fisting were not associated with (HG)AIN. ConclusionGHB use increases the risk for AIN, while duration of cART and anal XTC use are negatively correlated with AIN. Given the high prevalence of AIN in HIV+ MSM, these associations are not helpful to guide a screening program.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Risk Factors for the Presence of Anal Intraepithelial Neoplasia in HIV+ Men Who Have Sex with Men

et al. 2013

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“…Some studies have shown that an association between HPV viral load and cytology does exist, especially for grades 2 and 3 intraepithelial neoplasia in HIV-positive MSM. 35,36 Other studies have not yielded the same results. 37 However, the clinical significance of the HPV viral load has not been clearly established and further studies are needed.…”

Section: Discussionmentioning

confidence: 73%

Assessing Urine Human Papillomavirus Polymerase Chain Reaction Testing As a Tool for Screening Anal HPV Infection in HIV-Positive MSM

Lanoix

Pannier²,

Borel³

et al. 2012

AIDS Patient Care and STDs

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Multiple types of human papillomavirus (HPV) are responsible for most cervical cancers but also cause anal cancers-especially in HIV-positive patients. Furthermore, men who have sex with men (MSM) are twice as likely to develop anal cancers as non-MSM. A simple screening test for HPV infection would be useful in these patients. The aim of our study was to evaluate the detection of HPV by real-time polymerase chain reaction (PCR) in urine as a marker of anal infection in MSM. The study included 52 HIV-positive MSM treated at Amiens University Hospital (Amiens, France). After obtaining informed consent, we performed an anal swab and gathered 10 mL of first-void urine. Samples were extracted and amplified in a real-time PCR. Genotypes were determined with a PapilloCheck(®) system (Greiner Bio-One, Frickenhausen, Germany). The anal test was the gold standard for calculating the characteristics of the urine test. The sensitivity of the urine test for diagnosing anal HPV infection was 15%, the specificity was 66%, the positive predictive value was 87.5%, and negative predictive value was 4.5%. The prevalence of anal HPV infection in the study population was 94%. Genotype 42 was the most common. The anal HPV viral load was significantly lower in men in a stable relationship than in single men. However, there was no statistically significant relationship between anal viral load and anal intraepithelial lesions. We conclude that urine-based HPV is a poor predictor of anal HPV infection in HIV-positive MSM.

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Assessment of the possible inhibitory effect of carrageenan in human papillomavirus DNA testing by polymerase chain reaction amplification

Laurie

El‐Zein

Franco

et al. 2021

Journal of Medical Virology

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Episomal and integrated human papillomavirus type 16 loads and anal intraepithelial neoplasia in HIV-seropositive men

Abstract: High episomal HPV-16 load but not HPV-16 integration load measured by real-time PCR was associated with AIN-2,3.

Cited by 24 publications

References 47 publications

Risk Factors for the Presence of Anal Intraepithelial Neoplasia in HIV+ Men Who Have Sex with Men

Risk Factors for the Presence of Anal Intraepithelial Neoplasia in HIV+ Men Who Have Sex with Men

Assessing Urine Human Papillomavirus Polymerase Chain Reaction Testing As a Tool for Screening Anal HPV Infection in HIV-Positive MSM

Assessment of the possible inhibitory effect of carrageenan in human papillomavirus DNA testing by polymerase chain reaction amplification

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