Epinephrine induced platelet aggregation in rat platelet-rich plasma

Yun‐Choi, Hye Sook; Park, Kyung Mi; Pyo, Mi Kyung

doi:10.1016/s0049-3848(00)00353-4

Cited by 50 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, such a specific elevation of epinephrine aggregation has not been reported in other diseases. The presence of α 2 -adrenoceptors on human platelets has been previously reported, 21 and it is recognized that epinephrine aggregation is mediated by α 2 -adrenoceptors since it is antagonized by α 2 -antagonists but not by α 1 -antagonists. 21 These findings suggest that the platelets of IBD patients are potentially sensitive to sympathetic stimuli, even during the inactive phase.…”

Section: Discussionmentioning

confidence: 85%

Increased aggregation response of platelets in patients with inflammatory bowel disease

et al. 2006

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 85%

Increased aggregation response of platelets in patients with inflammatory bowel disease

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Platelets are populated with alpha 2-adrenoceptor and play a role similar to ADP and thrombin receptors in platelet aggregation. 10 Any defect in alpha 2-adrenoceptors manifests clinically as haemostatic abnormalities and alteration in number or affinity of alpha 2-adrenoceptors in platelet membrane lead to defective platelet function. 11 Under the threshold concentration of collagen with epinephrine as an agonist, the effect of many alpha 2-adrenoceptor antagonists on platelet aggregation has been explored.…”

Section: Discussionmentioning

confidence: 99%

“…11 Under the threshold concentration of collagen with epinephrine as an agonist, the effect of many alpha 2-adrenoceptor antagonists on platelet aggregation has been explored. 10,[12][13][14] We used yohimbine (a standard alpha 2 adrenoceptor antagonist) as a comparator control and measured the degree of platelet aggregation at baseline and 5 h which was compared with cleistanthins A and B. Even though the half-life of yohimbine is 0.6 h, it produces ten times more potent active metabolite called 11-OH yohimbine with a half-life of 6-7 h. 15 Hence the effect of yohimbine could be observed even after 5 h. In our study, yohimbine at 2 mg/kg dose has shown a significant inhibition of platelet aggregation after 5 h by blocking alpha 2-adrenoceptor in platelet.…”

Section: Discussionmentioning

confidence: 99%

Action of Cleistanthins A and B on Alpha Adrenoceptors in Rats

Lakshmanan¹,

Bobby²,

Raveendran³

2016

JYP

View full text Add to dashboard Cite

Objectives: Cleistanthins A and B (CAB), active phytochemicals present in Cleistanthus collinus, have been proven to have alpha 1-adrenoceptor blocking property. However action on alpha 2-adrenoceptor is not studied. We aimed to study the action of CAB on alpha 2-adrenoceptor by measuring platelet aggregation, insulin and blood glucose levels with yohimbine as a standard comparator control and alpha 1-adrenoceptor by measuring the blockade of relaxation induced by phenylephrine in the jejunum of Wistar albino rats. Materials and methods: Forty eight adult male Wistar rats were divided into eight drug groups of six each, namely control (CMC 0.5%), yohimbine 2 mg/kg, 10, 30 and 100 mg/kg doses of cleistanthin A and cleistanthin B. Blood samples were drawn at baseline, after 5 h and after high-epinephrine dose (5 mg/kg) from the retro-orbital sinus and used for estimation of platelet aggregation and plasma insulin levels. Blood glucose levels were estimated at six different time points. Degree of jejunal relaxation was studied by relaxing the acetylcholine pre-contracted jejunum with phenylephrine. Results: All groups, except control and cleistanthin A 10 mg/kg group, significantly inhibited the platelet aggregation when compared to the respective baseline values (p<0.05). No significant difference was observed between different drug groups in plasma insulin and blood glucose levels. With regard to phenylephrine induced jejunal relaxation, the median IC 50 for all three doses of cleistanthin A and cleistanthin B 100 mg/kg differ significantly from that of control (CMC 0.5%). Conclusion: CAB block both alpha 1 and 2-adrenoceptorand hence are non-selective alpha-adrenoceptor blockers.

show abstract

“…The local infusion of norepinephrine before releasing the tourniquet induces contraction of peripheral vessels, with a reduction of this peak. Moreover, norepinephrine is a platelet-stimulating agent since it causes aggregation of human platelets through α2-adrenoceptors [12]. These actions may explain the efficacy of this drug at reducing perioperative (contraction of peripheral vessels) and postoperative (haemostatic effect) blood loss.…”

Section: Discussionmentioning

confidence: 99%

Local infusion of norepinephrine reduces blood losses and need of transfusion in total knee arthroplasty

Gasparini

Papaleo

Pola

et al. 2006

International Orthopaedics (SICO

View full text Add to dashboard Cite

Blood loss after total knee arthroplasty (TKA) is often associated with cardiovascular complications and a high transfusion rate of allogenic blood. In our study we focused our attention on developing a new intra-surgical procedure that appears safe, easy to perform and effective in the reduction of bleeding in TKA. We evaluated 84 patients who underwent TKA and met our inclusion criteria; they were assigned to two groups: 55 controls in which a saline solution was used to wash the surgical field before tourniquet release, and a second group of 29 patients, in which a saline solution containing a low dose of norepinephrine was locally applied before tourniquet release. The local administration of a low dose of norepinephrine has induced a significant reduction of perioperative blood loss and blood transfusion requirements; in addition, this method was characterised by the absence of complications or adverse effects. In conclusion, our data suggest that intraoperative local administration of a low dose of norepinephrine could represent an effective and safe method of reducing blood loss and preventing blood transfusions in patients with TKA.

show abstract

Epinephrine induced platelet aggregation in rat platelet-rich plasma

Cited by 50 publications

References 27 publications

Increased aggregation response of platelets in patients with inflammatory bowel disease

Increased aggregation response of platelets in patients with inflammatory bowel disease

Action of Cleistanthins A and B on Alpha Adrenoceptors in Rats

Local infusion of norepinephrine reduces blood losses and need of transfusion in total knee arthroplasty

Contact Info

Product

Resources

About