Epinephrine effects on carbohydrate metabolism in catfish, Ictalurus melas

Ottolenghi, C; Puviani, A.C.; Baruffaldi, A.; Brighenti, L

doi:10.1016/0016-6480(84)90007-8

Cited by 22 publications

(7 citation statements)

References 21 publications

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“…Adrenaline stimulates glycogen phosphorylase activity and glycogenolysis via β-adrenergic receptors, linked to the cAMP signaling cascade (Jensen et al, 1999). A similar scenario probably occurs in trout, as adrenaline infusion into resting catfish and trout stimulates glycogen phosphorylase activity and glycogenolysis in white muscle (Ottolenghi et al, 1984;Frolow, 1999). This effect is probably mediated by cAMP-linked β-adrenergic receptors, which have recently been identified in trout white muscle (Lortie and Moon, 2003).…”

Section: Discussionmentioning

confidence: 96%

A regulatory role for cortisol in muscle glycogen metabolism in rainbow troutOncorhynchus mykissWalbaum

Milligan

2003

Journal of Experimental Biology

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SUMMARY To test the hypothesis that cortisol has a regulatory role in fish muscle glycogenesis post-exercise, rainbow trout were treated 1 h prior to exercise with either saline (control) or metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) to block cortisol synthesis. Following exercise(time 0), half of the metyrapone-treated fish received a single injection of cortisol, to mimic the post-exercise rise usually observed. Muscle glycogen and the relative activities of glycogen phosphorylase a (Phos a) and glycogen synthase I (GSase I), regulatory enzymes for glycogen resynthesis, were monitored 4 h post-exercise. Metyrapone treatment succeeded in blocking the post-exercise rise in plasma cortisol(17±2 vs 118±13 ng ml–1 in controls at time 0), and cortisol injection resulted in a larger and more prolonged cortisol increase than in controls (159±22 vs 121±14 ng ml–1 in controls at 1 h). Muscle glycogen was completely restored in the metyrapone-treated fish within 2 h after exercise(8.3±0.6 vs 8±0.7 μmol g–1pre-exercise), only partially restored in control fish at 4 h (5.4±01.4 vs 8.8±1.3 μmol g–1 pre-exercise), and not at all in cortisol-treated fish (1.0±0.5 μmol g–1at 4 h). The rapid glycogen resynthesis in the metyrapone-treated fish was associated with a more rapid inactivation of Phos a and stimulation of GSase I compared to controls. In cortisol-treated fish, Phos a activity persisted throughout 4 h post-exercise; there was also a significant stimulation of GSase I activity. As a consequence of dual activation of Phos a and GSase I, glycogen cycling probably occurred, thus preventing net synthesis. This explains why the post-exercise elevation of cortisol inhibits net glycogen synthesis in trout muscle.

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Section: Discussionmentioning

confidence: 96%

A regulatory role for cortisol in muscle glycogen metabolism in rainbow troutOncorhynchus mykissWalbaum

Milligan

2003

Journal of Experimental Biology

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“…It is generally thought that this hyperglycaemic response to stressful situations helps to protect and maintain the supply of glucose for the brain and other tissues. However, there is considerable evidence that, in addition to cortisol, the stressorlinked release of catecholamines from sympathetic neurons contributes to the hyperglycaemic response (Mazeaud et al, 1977;Ottolenghi et al, 1984). Recent work on mammalian species suggests that the development of hyperglycaemia is a result of the interplay not only between cortisol and catecholamines, but also between insulin, glucagon and P-endorphin (Halter et al, 1984;Vranic et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Cohort sampling, anaesthesia and stocking‐density effects on plasma cortisol, thyroid hormone, metabolite and ion levels in rainbow trout, Salmo gairdneri Richardson

Laidley

Leatherland

1988

Journal of Fish Biology

145

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The effect of serial removal of fish from aquaria, anaesthesia and stocking density on plasma cortisol, thyroid hormone, metabolite and ion levels was examined in rainbow trout, Sulmo gairheri, to determine the consequences of normal handling and maintenance procedures on the activity of the pituitary-adrenal and pituitary-thyroid axes in the species.The capture and handling associated with serial removal of fish from an aquarium resulted in a rapid elevation of plasma cortisol concentrations and a slower rise in plasma glucose, Mg2+ and Na' concentrations; plasma K t levels showed a transient fall shortly after commencing the sampling, followed by a significant increase.A lethal concentration of 3-aminobenzoic acid ethyl ester (MS 222) of 125 mgl-(but not a sublethal concentration of 62.5 ng 1-') prevented the capture stressor-associated changes in most of the measured parameters, including plasma cortisol levels, although plasma protein and ion concentrations were elevated in fish sampled after MS 222-anaesthesia.Stocking densities from 1 to 64 fish 80 I-' aquarium did not appear to effect changes in the measured parameters, except for plasma T 3 n 4 ratios which decreased with increasing stocking density. The exception was found in fish which were stocked in pairs: one member of each pair had a markedly elevated plasma cortisol level and there was a high incidence of mortality, probably related to aggressive social interaction.

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“…Catecholamines exert their action in catfish through a ~-adrenergic mechanism, which causes stimulation of cAMP dependent processes (Ottolenghi et al 1984b;Brighenti et al 1987a,b). In mammals, glucagon has a greater effect than epinephrine upon both glycogenolytic rate and tissue cAMP levels (Sokal et al 1964;Hems and Whitton 1980).…”

Section: Discussionmentioning

confidence: 99%

Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes

Ottolenghi

Puviani

Gavioli

et al. 1989

Fish Physiol Biochem

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Glycogenolytic effects of salmon and mammalian glucagons, salmon glucagon-like peptide (GLP) and epinephrine were studied on liver cells isolated from catfish (Ictalurus melas). In spring and summer, salmo-glucagon (3×10(-10) to 3×10(-8) M) was more effective than its mammalian counterpart in the stimulation of glucose release and cAMP synthesis in hepatocytes. GLP was less potent as compared to both glucagons. γ-amylase activity was not affected by the treatment with either glucagon-family peptides or epinephrine.The comparison of the glycogenolytic effects of salmon glucagon to those of epinephrine reveals a greater potency of the latter hormone in the stimulation of cAMP synthesis, glycogen-phosphorylase activity and glucose release. Glycogen content in the liver cells was equally depleted after treatment with both of the two hormones.

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Epinephrine effects on carbohydrate metabolism in catfish, Ictalurus melas

Cited by 22 publications

References 21 publications

A regulatory role for cortisol in muscle glycogen metabolism in rainbow troutOncorhynchus mykissWalbaum

A regulatory role for cortisol in muscle glycogen metabolism in rainbow troutOncorhynchus mykissWalbaum

Cohort sampling, anaesthesia and stocking‐density effects on plasma cortisol, thyroid hormone, metabolite and ion levels in rainbow trout, Salmo gairdneri Richardson

Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes

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