Epileptic Focus Stimulation and Seizure Control in the Rat Model of Kainic Acid-Induced Limbic Seizures

Urino, Tomoyuki; Hashizume, Kazuyoshi; Maehara, Michiyo; Kato, Kouichi; Okada, Yoshikazu; Hori, Tomokatsu; Tanaka, Tatsuya

doi:10.2176/nmc.50.355

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…The mediodorsal thalamic nucleus is thought to be involved in the modulation of seizures, specifically those involving limbic regions (Bertram et al, 1998(Bertram et al, , 2001. Previous clinical studies showed that electrical stimulation in MT can decrease seizure frequency (Sterman et al, 1982;Urino et al, 2010), and these results demonstrated that MT activities are involved in blocking seizures. Yet, the mechanisms by which thalamic stimulation through deep brain stimulation may reduce seizure frequency are not clear.…”

Section: Discussionmentioning

confidence: 98%

Spatiotemporal organization and thalamic modulation of seizures in the mouse medial thalamic-anterior cingulate slice

Chang

Lee

et al. 2011

Epilepsia

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SUMMARYPurpose: Seizure-like activities generated in anterior cingulate cortex (ACC) are usually classified as simple partial and are associated with changes in autonomic function, motivation, and thought. Previous studies have shown that thalamic inputs can modulate ACC seizure, but the exact mechanisms have not been studied thoroughly. Therefore, we investigated the role of thalamic inputs in modulating ACC seizure-like activities. In addition, seizure onset and propagation are difficult to determine in vivo in ACC. We studied the spatiotemporal changes in epileptiform activity in this cortex in a thalamic-ACC slice to clearly determine seizure onset. Methods: We used multielectrode array (MEA) recording and calcium imaging to investigate the modulatory effect of thalamic inputs in a thalamic-ACC slice preparation. Key Findings: Seizure-like activities induced with 4-aminopyridine (4-AP; 250 lM) and bicuculline (5-50 lM) in ACC were attenuated by glutamate receptor antagonists, and the degree of disinhibition varied with the dose of bicuculline. Seizure-like activities were decreased with 1 Hz thalamic stimulation, whereas corpus callosum stimulation could increase ictal discharges. Amplitude and duration of cingulate seizure-like activities were augmented after removing thalamic inputs, and this effect was not observed with those induced with elevated bicuculline (50 lM). Seizure-like activities were initiated in layers II/III and, after thalamic lesions, they occurred mainly in layers V/VI. Two-dimensional current-source density analyses revealed sink signals more frequently in layers V/VI after thalamic lesions, indicating that these layers produce larger excitatory synchronization. Calcium transients were synchronized after thalamic lesions suggesting that ACC seizure-like activities are subjected to desynchronizing modulation by thalamic inputs. Therefore, ACC seizure-like activities are subject to desynchronizing modulation from medial thalamic inputs to deep layer pyramidal neurons. Significance: Cingulate seizure-like activities were modulated significantly by thalamic inputs. Repeated stimulation of the thalamus efficiently inhibited epileptiform activity, demonstrating that the desynchronization was pathway-specific. The clinical implications of deep thalamic stimulation in the modulation of cingulate epileptic activity require further investigation.

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Section: Discussionmentioning

confidence: 98%

Spatiotemporal organization and thalamic modulation of seizures in the mouse medial thalamic-anterior cingulate slice

Chang

Lee

et al. 2011

Epilepsia

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“…The acquired chronic epileptic state in this rat model for TLE makes it a good model to study the effects of anti-epileptic treatments on the occurrence rate of spontaneous epileptic seizures. Several preclinical studies investigating the effect of high frequency hippocampal DBS are performed in animal models where seizures are evoked either after kindling stimuli [4,5], or after injection of chemo-convulsants [6,7,32]. Only a few pre-clinical studies have investigated the effect of hippocampal DBS on the occurrence of spontaneous seizures in the chronic epileptic state [3,30,31].…”

Section: Discussionmentioning

confidence: 99%

In Search of Optimal DBS Paradigms to Treat Epilepsy: Bilateral Versus Unilateral Hippocampal Stimulation in a Rat Model for Temporal Lobe Epilepsy

Nieuwenhuyse¹,

Raedt²,

Delbeke³

et al. 2015

Brain Stimulation

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“…Following injection of kainic acid into rat amygdala, producing acute status epilepticus, 10 Hz stimulation of the amygdala and 130 Hz stimulation of the dorsal thalamus inhibited seizures, but 10 Hz ventral hippocampal stimulation had no effect (Urino et al, 2010). Following injection of kainic acid into rat amygdala, producing acute status epilepticus, 10 Hz stimulation of the amygdala and 130 Hz stimulation of the dorsal thalamus inhibited seizures, but 10 Hz ventral hippocampal stimulation had no effect (Urino et al, 2010).…”

Section: Hippocampal Stimulation For Epilepsy: Animal Studiesmentioning

confidence: 97%

Deep brain stimulation for epilepsy

Fisher

2013

Handbook of Clinical Neurology

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Epileptic Focus Stimulation and Seizure Control in the Rat Model of Kainic Acid-Induced Limbic Seizures

Cited by 8 publications

References 26 publications

Spatiotemporal organization and thalamic modulation of seizures in the mouse medial thalamic-anterior cingulate slice

Spatiotemporal organization and thalamic modulation of seizures in the mouse medial thalamic-anterior cingulate slice

In Search of Optimal DBS Paradigms to Treat Epilepsy: Bilateral Versus Unilateral Hippocampal Stimulation in a Rat Model for Temporal Lobe Epilepsy

Deep brain stimulation for epilepsy

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