Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea

Chandana, B. S.; Mahto, Rohit Kumar; Singh, Rajesh Kumar; Ford, Rebecca; Vaghefi, Niloofar; Gupta, Santosh Kumar; Yadav, Hemant Kumar; Manohar, Murli; Kumar, Rajendra

doi:10.3389/fgene.2022.900253

Cited by 7 publications

(6 citation statements)

References 247 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, plants that are subjected to dryness may compress chromatin around genes involved in photosynthesis. This aids in energy conservation by preventing these genes from being activated [22]. Histone acetylation and methylation are mediated by a number of epigenetic regulators, including acetyltransferases, methyltransferases, deacetylases, and demethylases, during the heat stress response [23].…”

Section: Foundations Of Epigenetics In Plantsmentioning

confidence: 99%

Unveiling the epigenetic landscape of plants using flow cytometry approach

Jyoti,

Chandel,

Singh

2024

Cytometry Pt A

View full text Add to dashboard Cite

Plants are sessile creatures that have to adapt constantly changing environmental circumstances. Plants are subjected to a range of abiotic stressors as a result of unpredictable climate change. Understanding how stress‐responsive genes are regulated can help us better understand how plants can adapt to changing environmental conditions. Epigenetic markers that dynamically change in response to stimuli, such as DNA methylation and histone modifications are known to regulate gene expression. Individual cells or particles' physical and/or chemical properties can be measured using the method known as flow cytometry. It may therefore be used to evaluate changes in DNA methylation, histone modifications, and other epigenetic markers, making it a potent tool for researching epigenetics in plants. We explore the use of flow cytometry as a technique for examining epigenetic traits in this thorough discussion. The separation of cell nuclei and their subsequent labeling with fluorescent antibodies, offering information on the epigenetic mechanisms in plants when utilizing flow cytometry. We also go through the use of high‐throughput data analysis methods to unravel the complex epigenetic processes occurring inside plant systems.

show abstract

Section: Foundations Of Epigenetics In Plantsmentioning

confidence: 99%

Unveiling the epigenetic landscape of plants using flow cytometry approach

Jyoti,

Chandel,

Singh

2024

Cytometry Pt A

View full text Add to dashboard Cite

show abstract

“…Further, correlation between transcriptome (eQTL) and methylome (meQTLs) have been established for genetic regulation of complex traits ( Oliva et al, 2022 ). In addition, a better comprehension of and capacity to choose advantageous epigenomic modifications is suggested to incorporate a more effective and comprehensive approach to crop improvement ( AieseCigliano et al, 2022 ; Chandana et al, 2022 ). This is because many plant stress responses are governed by epigenomic processes, notably through cell-autonomous epigenetic switching.…”

Section: Strategies To Overcome Limitations Of Microgreen Productionmentioning

confidence: 99%

Prospects of microgreens as budding living functional food: Breeding and biofortification through OMICS and other approaches for nutritional security

Gupta

Sharma

Singh³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Nutrient deficiency has resulted in impaired growth and development of the population globally. Microgreens are considered immature greens (required light for photosynthesis and growing medium) and developed from the seeds of vegetables, legumes, herbs, and cereals. These are considered “living superfood/functional food” due to the presence of chlorophyll, beta carotene, lutein, and minerals like magnesium (Mg), Potassium (K), Phosphorus (P), and Calcium (Ca). Microgreens are rich at the nutritional level and contain several phytoactive compounds (carotenoids, phenols, glucosinolates, polysterols) that are helpful for human health on Earth and in space due to their anti-microbial, anti-inflammatory, antioxidant, and anti-carcinogenic properties. Microgreens can be used as plant-based nutritive vegetarian foods that will be fruitful as a nourishing constituent in the food industryfor garnish purposes, complement flavor, texture, and color to salads, soups, flat-breads, pizzas, and sandwiches (substitute to lettuce in tacos, sandwich, burger). Good handling practices may enhance microgreens’stability, storage, and shelf-life under appropriate conditions, including light, temperature, nutrients, humidity, and substrate. Moreover, the substrate may be a nutritive liquid solution (hydroponic system) or solid medium (coco peat, coconut fiber, coir dust and husks, sand, vermicompost, sugarcane filter cake, etc.) based on a variety of microgreens. However integrated multiomics approaches alongwith nutriomics and foodomics may be explored and utilized to identify and breed most potential microgreen genotypes, biofortify including increasing the nutritional content (macro-elements:K, Ca and Mg; oligo-elements: Fe and Zn and antioxidant activity) and microgreens related other traits viz., fast growth, good nutritional values, high germination percentage, and appropriate shelf-life through the implementation of integrated approaches includes genomics, transcriptomics, sequencing-based approaches, molecular breeding, machine learning, nanoparticles, and seed priming strategiesetc.

show abstract

“…CH is a high heritability trait, as effective as yield (Singh et al, 2005). Higher heritability indicates that the environment has less in uence on the trait, and further describes the value of breeding (Oumata et al, 2022;Chandana et al, 2022). Several studies have already veri ed the potential of CH from many 3D sensing platforms, including the use of LiDAR (Kronenberg et al, 2017;Walter et al, 2019) and UAV imagery (Volpato et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Field-measured canopy height may not be as accurate and heritable as believed – Evidence from advanced 3D sensing

Zang

Jin

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Canopy height (CH) is an important trait for crop breeding and production. The rapid development of 3D sensing technologies shed new light on high-throughput height measurement. However, a systematic comparison of the accuracy and heritability of different 3D sensing technologies is seriously lacking. Moreover, it is questionable whether the field-measured height is as reliable as believed. This study uncovered these issues by comparing traditional height measurement with four advanced 3D sensing technologies, including terrestrial laser scanning (TLS), backpack laser scanning (BLS), gantry laser scanning (GLS), and digital areal photogrammetry (DAP). A total of 1920 plots covering 120 varieties were selected for comparison. Cross-comparisons of different data sources were performed to evaluate their performances in CH estimation concerning different CH, leaf area index (LAI), and growth stage (GS) groups. Results showed that 1) All 3D sensing data sources had high correlations with field measurement (r>0.82), while the correlations between different 3D sensing data sources were even better (r>0.87). 2) The prediction accuracy between different data sources decreased in subgroups of CH, LAI, and GS. 3) Canopy height showed high heritability from all datasets, and 3D sensing datasets had even higher heritability (H2=0.79-0.89) than FM (H2=0.77). Finally, outliers of different datasets are analyzed. The results provide novel insights into different methods for canopy height measurement that may ensure the high-quality application of this important trait.

show abstract

Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea

Cited by 7 publications

References 247 publications

Unveiling the epigenetic landscape of plants using flow cytometry approach

Unveiling the epigenetic landscape of plants using flow cytometry approach

Prospects of microgreens as budding living functional food: Breeding and biofortification through OMICS and other approaches for nutritional security

Field-measured canopy height may not be as accurate and heritable as believed – Evidence from advanced 3D sensing

Contact Info

Product

Resources

About