Epigenome-wide association analysis of pancreatic exocrine cells from high-fat- and normal diet-fed mice and its potential use for understanding the oncogenesis of human pancreatic cancer

Selective epigenetic alterations in RNF43 in pancreatic exocrine cells from high-fat-diet-induced obese mice; implications for pancreatic cancer

“…All procedures for the experiments using mice were approved by the Animal Committee of Saitama Medical University (protocol:2499). All methods were performed following experimental procedures mirroring those described in our previous study [ 25 ]. Briefly, mice were euthanized at approximately 60-week old with CO 2 gas, and pancreatic tissues were excised.…”

“…In our recent study, we performed an epigenome-wide analysis of isolated pancreatic exocrine cells obtained from high fat-diet (HFD)-induced obesity (DIO) mice. Obesity induces whole-genome scale abnormalities in DNA methylation in the presymptomatic stage, with enrichment in cellular processes, such as DNA repair, transcription regulation, and cell proliferation [ 25 ]. Comparing differentially methylated regions (DMRs) with those in stage IB PC, we identified three potential pathway candidates for PC development.…”