“…Major evidence, albeit primarily in murine models, indicates a central role of miR-mediated posttranscriptional regulation in MDSC differentiation and function (40,51). Via in vitro studies on monocyte conditioning, we found that hallmarks of human MDSCs, i.e., downregulation of HLA-DR, secretion of IL-6, TGF-β, and CCL2, and inhibition of T cell proliferation and function (9, 10), can be reconstituted by in vitro cotransfection of the 8 MDSC-miRs; this finding held true even when the requirement was decreased to 4 miRs (miR-146a, miR-100, miR-125b, and miR-155) in some donors (data not shown).…”