Epigenetic regulations as drivers of insecticide resistance and resilience to climate change in arthropod pests

Mogilicherla, Kanakachari; Roy, Amit

doi:10.3389/fgene.2022.1044980

Cited by 5 publications

References 188 publications

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Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis

Liu,

Li,

Zhao

et al. 2024

Cell Biology International

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Cisplatin is commonly used for the chemotherapy of tongue squamous cell carcinoma (TSCC); however, adverse side effects and drug resistance impact its therapeutic efficacy. Capsaicin is an active ingredient in chili peppers that exerts antitumor effects, whether it exerts antitumor effects on cisplatin‐resistant cells remains unknown. Therefore, in this study, we investigated the effect of capsaicin on cisplatin resistance in TSCC cells and explored the underlying mechanisms. A cisplatin‐resistant TSCC cell line was established by treated with increasing cisplatin concentrations. Combined treatment with cisplatin and capsaicin decreased the glucose consumption and lactate dehydrogenase activity and increased the adenosine triphosphate production both in vitro and in vivo, suggesting the inhibition of the Warburg effect. Moreover, this combined treatment induced cell apoptosis and significantly upregulated the levels of proapoptotic proteins, such as Bax, cleaved caspase‐3, ‐7, and ‐9, and apoptosis‐inducing factor. In contrast, levels of the antiapoptotic protein, Bcl‐2, were downregulated. Additionally, LKB1 and AMPK activities were stimulated, whereas those of AKT and mTOR were suppressed. Notably, AMPK knockdown abolished the inhibitory effects of capsaicin and cisplatin on the AKT/mTOR signaling pathway and Warburg effect. Overall, combined treatment with capsaicin and cisplatin reversed cisplatin resistance by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis. Our findings suggest combination therapy with capsaicin and cisplatin as a potentially novel strategy and highlight capsaicin as a promising adjuvant drug for TSCC treatment.

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Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis

Liu,

Li,

Zhao

et al. 2024

Cell Biology International

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An optical system to detect, surveil, and kill flying insect vectors of human and crop pathogens

Patt,

Makagon,

Norton

et al. 2024

Sci Rep

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Sustainable and effective means to control flying insect vectors are critically needed, especially with widespread insecticide resistance and global climate change. Understanding and controlling vectors requires accurate information about their movement and activity, which is often lacking. The Photonic Fence (PF) is an optical system that uses machine vision, infrared light, and lasers to identify, track, and interdict vectors in flight. The PF examines an insect’s outline, flight speed, and other flight parameters and if these match those of a targeted vector species, then a low-power, retina-safe laser kills it. We report on proof-of-concept tests of a large, field-sized PF (30 mL × 3 mH) conducted with Aedes aegypti, a mosquito that transmits dangerous arboviruses, and Diaphorina citri, a psyllid which transmits the fatal huanglongbing disease of citrus. In tests with the laser engaged, < 1% and 3% of A. aegypti and D. citri, respectfully, were recovered versus a 38% and 19% recovery when the lacer was silenced. The PF tracked, but did not intercept the orchid bee, Euglossa dilemma. The system effectively intercepted flying vectors, but not bees, at a distance of 30 m, heralding the use of photonic energy, rather than chemicals, to control flying vectors.

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Oligonucleotide insecticides (contact unmodified antisense DNA biotechnology) and RNA biocontrols (double-stranded RNA technology): newly born fraternal twins in plant protection

Oberemok,

Gal’chinsky

2024

Preprint

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The rapid development of molecular genetics, methods of synthesis of nucleic acids, and bioinformatics have created fundamentally new opportunities in plant protection. DNA and RNA have become active ingredients of insecticides, going beyond their usual role as carriers of hereditary information. Now nucleic acids are increasingly acting as active tools for insect pest control using the mechanisms of DNA containment (oligonucleotide insecticides, contact unmodified antisense DNA (CUAD) biotechnology) and RNA interference (RNA biocontrols, double-stranded RNA biotechnology). Partial understanding of how antisense DNA oligonucleotides might work began in the late 1970s of XX century and for a long time (until the beginning of the XXI century) these biomolecules had not been implemented in plant protection in the form of any biotechnology based on unmodified or modified DNA fragments. RNA interference was discovered in the late 90s of XX century and also went through quite a long path of development in plant protection before the first encouraging results of its application as insecticides appeared. Both, CUAD biotechnology and double-stranded RNA technology aim to inactivate the expression of genes of insect pests using antisense DNA and double-stranded RNA that help trigger degradation of the target RNA. As two new classes of the next-generation insecticides, these products are supposed to have long operational life, low carbon footprint, high selectivity in action and rapid biodegradation in ecosystems. To date, antisense CUAD biotechnology shows the best results on sap-sucking insects while double-stranded RNA technology shows the best insecticidal potential on beetles from Tenebrionidae and Chrysomelidae families. With a high probability, oligonucleotide insecticides and RNA biocontrols, will be effective against certain groups of insects and can complement each other in complex formulations for control of wide range of pests.

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Epigenetic regulations as drivers of insecticide resistance and resilience to climate change in arthropod pests

Cited by 5 publications

References 188 publications

Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis

Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis

An optical system to detect, surveil, and kill flying insect vectors of human and crop pathogens

Oligonucleotide insecticides (contact unmodified antisense DNA biotechnology) and RNA biocontrols (double-stranded RNA technology): newly born fraternal twins in plant protection

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