Epigenetic regulation of mammalian sex determination

Tachibana, Makoto

doi:10.2152/jmi.62.19

Cited by 16 publications

(7 citation statements)

References 48 publications

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“…Recently, epigenetic mechanisms have been demonstrated to regulate genes involved in sex determination and gonadal differentiation (Kuroki et al, 2013;Piferrer, 2013;Shao et al, 2014;Tachibana, 2015;Zhang et al, 2013). Epigenetic mechanisms, including DNA methylation, histone modification and non-coding RNAs, can integrate environmental information with the regulation of gene expression, and have been emerging as a promising regulatory mechanism for TSD (Matsumoto et al, 2013a;Navarro-Martín et al, 2011;Parrott et al, 2014;Piferrer, 2013;Venegas et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Dmrt1 induces the male pathway in a turtle with temperature-dependent sex determination

Ye²,

Zhang

et al. 2017

Development

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The molecular mechanism underlying temperature-dependent sex determination (TSD) has been a long-standing mystery; in particular, the thermosensitive genetic triggers for gonadal sex differentiation are largely unknown. Here, we have characterized a conserved DM domain gene, Dmrt1, in the red-eared slider turtle Trachemys scripta (T. scripta), which exhibits TSD. We found that Dmrt1 has a temperature-dependent, sexually dimorphic expression pattern, preceding gonadal sex differentiation, and is capable of responding rapidly to temperature shifts and aromatase inhibitor treatment. Most importantly, loss-and gain-of-function analyses provide solid evidence that Dmrt1 is both necessary and sufficient to initiate male development in T. scripta. Furthermore, the DNA methylation dynamics of the Dmrt1 promoter are tightly correlated with temperature and could mediate the impact of temperature on sex determination. Collectively, our findings demonstrate that Dmrt1 is a candidate master male sex-determining gene in this TSD species, consistent with the idea that DM domain genes are conserved during the evolution of sex determination mechanisms.

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Section: Discussionmentioning

confidence: 99%

Dmrt1 induces the male pathway in a turtle with temperature-dependent sex determination

Ye²,

Zhang

et al. 2017

Development

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“…In forensic medicine, DNA methylation is typically chosen over other epigenetic alterations because of its in vitro stability and great sensitivity in terms of the amount of DNA required [ 205 ]. Based on the notion that environmental stimuli can cause individual epigenomic variations [ 205 , 206 ], forensic investigations apply DNA methylation to the identification of tissues and cells [ 207 ], to the determination of both sex and age of individuals [ 208 , 209 ], and to the differentiation between monozygotic twins [ 210 ]. Furthermore, DNA methylation can disclose information about a person’s socioeconomic status, diet, physical activity, alcohol use [ 211 ], smoking status and drug use [ 212 ], and thus may aid in the discovery of specific markers that can provide more detailed information about the characteristics and effects of NPSs.…”

Section: New Perspectives In the Forensic Fieldmentioning

confidence: 99%

Epigenetic Studies for Evaluation of NPS Toxicity: Focus on Synthetic Cannabinoids and Cathinones

et al. 2022

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In the recent decade, numerous new psychoactive substances (NPSs) have been added to the illicit drug market. These are synthetized to mimic the effects of classic drugs of abuse (i.e., cannabis, cocaine, etc.), with the purpose of bypassing substance legislations and increasing the pharmacotoxicological effects. To date, research into the acute pharmacological effects of new NPSs is ongoing and necessary in order to provide an appropriate contribution to public health. In fact, multiple examples of NPS-related acute intoxication and mortality have been recorded in the literature. Accordingly, several in vitro and in vivo studies have investigated the pharmacotoxicological profiles of these compounds, revealing that they can cause adverse effects involving various organ systems (i.e., cardiovascular, respiratory effects) and highlighting their potential increased consumption risks. In this sense, NPSs should be regarded as a complex issue that requires continuous monitoring. Moreover, knowledge of long-term NPS effects is lacking. Because genetic and environmental variables may impact NPS responses, epigenetics may aid in understanding the processes behind the harmful events induced by long-term NPS usage. Taken together, “pharmacoepigenomics” may provide a new field of combined study on genetic differences and epigenetic changes in drug reactions that might be predictive in forensic implications.

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“…In genetic sex determination (GSD) species, promoter methylation in diacylglycerol kinase delta gene (Dgkd) has been found to be involved in Pacific oyster (Crassostrea gigas) sex determination [7]. Sry gene has been found to play a key role in mammal sex determination and its hypomethylation had been reported to promote testis development [8].…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation Difference between Female and Male Ussuri Catfish (Pseudobagrus ussuriensis) in Brain and Gonad Tissues

Chen

Zhu

et al. 2022

Life

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DNA methylation has been found to be involved in sex determination and differentiation in many aquaculture species. The Ussuri catfish (Pseudobagrus ussuriensis) is a popular aquaculture fish in China with high economic value in which male-biased sex dimorphism was observed in terms of body size and body weight. In this study, DNA methylation-sensitive RAD sequencing (Methyl-RAD) was used to explore the epigenetic difference between adult male and female samples in brain and gonad tissues. In brain tissues, 5,442,496 methylated cytosine sites were found and 9.94% of these sites were from symmetric CCGG or CCWGG sites. Among these sites, 321 differential DNA methylation sites (DMSs) in 171 genes were identified, while in gonad tissues, 4,043,053 methylated cytosines sites were found in total and 11.70% of them were from CCGG or CCWGG. Among these sites, 78 differential DNA methylation sites were found which were located in 64 genes. We also found several sex-determination genes among these differential methylated genes, such as amh, gsdf and hsd11b2 in brain tissues and slco3a1, socs2 and trim47 in gonad tissues. These results provided evidence for understanding the function of DNA methylation in the sex differentiation in Pseudobagrus ussuriensis, which further deepens the relationship between gene regulation and epigenetics.

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Epigenetic regulation of mammalian sex determination

Cited by 16 publications

References 48 publications

Dmrt1 induces the male pathway in a turtle with temperature-dependent sex determination

Dmrt1 induces the male pathway in a turtle with temperature-dependent sex determination

Epigenetic Studies for Evaluation of NPS Toxicity: Focus on Synthetic Cannabinoids and Cathinones

DNA Methylation Difference between Female and Male Ussuri Catfish (Pseudobagrus ussuriensis) in Brain and Gonad Tissues

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