Epigenetic regulation by polycomb group complexes: focus on roles of CBX proteins

Ma, Rong-gang; Zhang, Yang; Sun, Tingting; Cheng, Bo

doi:10.1631/jzus.b1400077

Cited by 79 publications

(88 citation statements)

References 114 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also found that HP1α regulates several angiogenesis‐related genes in EPCs. HP1α is a constituent of mammalian epigenetic inhibitory complexes that typically maintain a repressive status of local chromosome structure . However, in our experimental system, an increase in HP1α expression during EPC differentiation was associated with the increased expression of genes that are known to have important functions in angiogenesis, and the knockdown of HP1α in OECs downregulated these genes.…”

Section: Discussionmentioning

confidence: 62%

Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells

et al. 2015

View full text Add to dashboard Cite

As the ability to control the differentiation of endothelial stem/progenitor cells (EPCs) into vascular endothelial cell lineages could be useful for promoting neovascularization, it is important to obtain a deeper understanding of the epigenetic mechanisms that regulate EPC differentiation and neovascularization. Heterochromatin protein 1a (HP1a) is known to be involved in the epigenetic regulation of gene silencing. However, recent reports demonstrate that HP1a can also activate gene expression during cell differentiation. In this study, microarray analysis revealed that HP1a expression was induced during EPC differentiation and is associated with the expression of outgrowing endothelial cell (OEC)-specific protein markers. To explore the role of HP1a in the differentiation of EPCs to OECs, its expression was knocked-down or overexpressed in differentiating EPCs. Overexpression of HP1a promoted the differentiation and angiogenic activity of EPCs in vitro and in vivo, whereas knockdown of HP1a led to a defect in OEC migration, tube formation, and angiogenic sprouting activity. Gene expression profiling showed increased expression of angiogenic genes, including NOTCH1, cadherin-5, and angiopoietin-like-2, and decreased expression of progenitor cell marker genes, including CD133, CXCR4, and C-KIT, in HP1a-overexpressing EPCs. Also, increased HP1a at an early stage of EPC differentiation may regulate angiogenic gene transcription by interacting with chromatin that modifies epigenetic factors such as the methyl-CpG binding domain, Polycomb group ring finger 2, and DNA methyltransferases. Our findings demonstrate, for the first time, that HP1a plays an important role in the differentiation and angiogenic function of EPCs by regulating endothelial gene expression. STEM CELLS 2015;33:1512-1522

show abstract

Section: Discussionmentioning

confidence: 62%

Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Polycombgroup (PCG) proteins were originally discovered in Drosophila and are involved in the development of the body segments of Drosophila (Lewis, 1978;Orlando, Jane, Chinwalla, Harte, & Paro, 1998). In mammals, PCG proteins are key regulatory factors of many developmental genes (Ma, Zhang, Sun, & Cheng, 2014). PCGF5 is a promoting factor for the SMAD2/TGF-β signalling pathway and for neural differentiation, with findings reveal a critical context-specific function for PCGF5 in directing PRC1 to control cell fate (Yao et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes

Wang

Zhang

Gan

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non‐oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up‐regulated and 21 were down‐regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac‐related pathways, such as ‘dilated cardiomyopathy’, ‘hypertrophic cardiomyopathy’, ‘cardiac muscle contraction’ and ‘adrenergic signalling in cardiomyopathy’. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus.

show abstract

“…By definition, all PRC1s consist of homologs of these four core members we call "canonical" (cPRC1). The composition, targeting and activity of the various cPRC1s changes dynamically during embryonic development, cell differentiation or in cancer (reviewed in [184][185][186][187]). This is in accordance with the notion that PcG complexes are involved in repressing several thousand genes in mammalian genomes, and the pool of regulatory target genes are not simply tissue specific, but it is changing spatiotemporally as differentiation proceeds (reviewed in [188]).…”

Section: Canonical Prc1 Complexesmentioning

confidence: 99%

From Flies to Mice: The Emerging Role of Non-Canonical PRC1 Members in Mammalian Development

2018

View full text Add to dashboard Cite

Originally two types of Polycomb Repressive Complexes (PRCs) were described, canonical PRC1 (cPRC1) and PRC2. Recently, a versatile set of complexes were identified and brought up several dilemmas in PRC mediated repression. These new class of complexes were named as non-canonical PRC1s (ncPRC1s). Both cPRC1s and ncPRC1s contain Ring finger protein (RING1, RNF2) and Polycomb group ring finger catalytic (PCGF) core, but in ncPRCs, RING and YY1 binding protein (RYBP), or YY1 associated factor 2 (YAF2), replaces the Chromobox (CBX) and Polyhomeotic (PHC) subunits found in cPRC1s. Additionally, ncPRC1 subunits can associate with versatile accessory proteins, which determine their functional specificity. Homozygous null mutations of the ncPRC members in mice are often lethal or cause infertility, which underlines their essential functions in mammalian development. In this review, we summarize the mouse knockout phenotypes of subunits of the six major ncPRCs. We highlight several aspects of their discovery from fly to mice and emerging role in target recognition, embryogenesis and cell-fate decision making. We gathered data from stem cell mediated in vitro differentiation assays and genetically engineered mouse models. Accumulating evidence suggests that ncPRC1s play profound role in mammalian embryogenesis by regulating gene expression during lineage specification of pluripotent stem cells.

show abstract

Epigenetic regulation by polycomb group complexes: focus on roles of CBX proteins

Cited by 79 publications

References 114 publications

Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells

Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes

From Flies to Mice: The Emerging Role of Non-Canonical PRC1 Members in Mammalian Development

Contact Info

Product

Resources

About