Epigenetic modulation and apoptotic induction by a novel imidazo-benzamide derivative in human lung adenocarcinoma cells

Arjunan, Amrutha; Pajaniradje, Sankar; Francis, Arul Prakash; Subramanian, Srividya; Chandramohan, Sathyapriya; Parthasarathi, D.; Sajith, Ayyiliath M.; Padusha, M. Syed Ali; Mathur, P. B.; Rajagopalan, Rukkumani

doi:10.1007/s40199-021-00419-3

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…This indicates the apoptosis induced by the formulation. Live cells appear bright green, and early to late apoptotic cells appear with yellow, orange and red nuclei [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…To detect apoptotic cells with condensed chromatin, the acridine orange (AO) and ethidium bromide (EB) dual staining method was employed as per Arjunan et al (2021) [ 16 ]. Briefly, 1 × 10 5 cells/mL of SK-MEL-3 cells was seeded in 24-well plates and was with sub-optimal concentrations of doxorubicin, RC, ACV and RC+ACV for 24 h. After the treatment, apoptotic cells took up EB, emitting red/orange fluorescence under 550 nm.…”

Section: Methodsmentioning

confidence: 99%

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Development of a Novel Red Clay-Based Drug Delivery Carrier to Improve the Therapeutic Efficacy of Acyclovir in the Treatment of Skin Cancer

Francis,

Ahmad,

Nagarajan

et al. 2023

Pharmaceutics

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Acyclovir (ACV) is a promising candidate for drug repurposing because of its potential to provide an effective treatment for viral infections and non-viral diseases, such as cancer, for which limited treatment options exist. However, its poor physicochemical properties limit its application. This study aimed to formulate and evaluate an ACV-loaded red clay nanodrug delivery system exhibiting an effective cytotoxicity. The study focused on the preparation of a complex between ACV and red clay (RC) using sucrose stearate (SS) (nanocomplex F1) as an immediate-release drug-delivery system for melanoma treatment. The synthesized nanocomplex, which had nanosized dimensions, a negative zeta potential and the drug release of approximately 85% after 3 h, was found to be promising. Characterization techniques, including FT-IR, XRD and DSC-TGA, confirmed the effective encapsulation of ACV within the nanocomplex and its stability due to intercalation. Cytotoxicity experiments conducted on melanoma cancer cell lines SK-MEL-3 revealed that the ACV release from the nanocomplex formulation F1 effectively inhibited the growth of melanoma cancer cells, with an IC50 of 25 ± 0.09 µg/mL. Additionally, ACV demonstrated a significant cytotoxicity at approximately 20 µg/mL in the melanoma cancer cell line, indicating its potential repurposing for skin cancer treatment. Based on these findings, it can be suggested that the RC-SS complex could be an effective drug delivery carrier for localized cancer therapy. Furthermore, the results of an in silico study suggested the addition of chitosan to the formulation for a more effective drug delivery. Energy and interaction analyses using various modules in a material studio demonstrated the high stability of the composite comprising red clay, sucrose stearate, chitosan and ACV. Thus, it could be concluded that the utilization of the red clay-based drug delivery system is a promising strategy to improve the effectiveness of targeted cancer therapy.

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“…This indicates the apoptosis induced by the formulation. Live cells appear bright green, and early to late apoptotic cells appear with yellow, orange and red nuclei [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Development of a Novel Red Clay-Based Drug Delivery Carrier to Improve the Therapeutic Efficacy of Acyclovir in the Treatment of Skin Cancer

Francis,

Ahmad,

Nagarajan

et al. 2023

Pharmaceutics

Self Cite

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Enhancing the fisetin efficacy through encapsulation with polymannose and nanotization for effective anticancer therapy towards breast cancer

Yadav,

Francis,

Chandramohan

et al. 2024

J Nanopart Res

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PHD3 as a Prognosis Marker and its Relationship with Immune Infiltration in Lung Adenocarcinoma

Wang

Zhong

Wang

et al. 2022

CCHTS

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Background: Lung adenocarcinoma (LUAD) is a highly heterogeneous malignant tumor. Therefore, it is necessary to find predictive biomarkers related to the prognosis and immune infiltration of lung adenocarcinoma, which may provide an effective theoretical basis for clinical treatment Objective: This study aimed to evaluate whether the expression level of PHD3 in lung adenocarcinoma (LUAD) was related to immunity. Method: PHD3 expression was analyzed by the ONCOMINE, TIMER, UALCAN, and GEPIA database. The correlations between clinical information and PHD3 expression were analyzed by LinkedOmics database. Then, we evaluated the influence of PHD3 on the survival of LUAD patients by Kaplan-Meier Plotter and HPA database. We explore the correlation between PHD3 and tumor immunity using TIMER and correlation module of TISDIB. Finally, we used the cBioportal database to analyze PHD3 mutations in LUAD Results: Comprehensive analysis displayed that PHD3 expression was clearly higher in LUAD compared with adjacent normal tissues. PHD3 expression was identified to be positively associated with tumor purity, histological type, and later pathological stage. Survival curve results revealed that the high expression of PHD3 in LUAD patients was accompanied via poor prognosis. Further study indicated that PHD3 was significantly related to a variety of tumor immune cells and molecules. Moreover, among the LUAD cases with gene alteration of PHD3, amplification was the most common of all alteration types Conclusion: PHD3 may be used as biomarkers for survival and immunotherapy of LUAD.

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Epigenetic modulation and apoptotic induction by a novel imidazo-benzamide derivative in human lung adenocarcinoma cells

Cited by 3 publications

References 33 publications

Development of a Novel Red Clay-Based Drug Delivery Carrier to Improve the Therapeutic Efficacy of Acyclovir in the Treatment of Skin Cancer

Development of a Novel Red Clay-Based Drug Delivery Carrier to Improve the Therapeutic Efficacy of Acyclovir in the Treatment of Skin Cancer

Enhancing the fisetin efficacy through encapsulation with polymannose and nanotization for effective anticancer therapy towards breast cancer

PHD3 as a Prognosis Marker and its Relationship with Immune Infiltration in Lung Adenocarcinoma

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