Epigenetic modification cooperates with Zeb1 transcription factor to regulate Bmp4 to promote chicken PGCs formation

Zhou, Shujian; Li, Tingting; Zhang, Ming; Chen, Chen; Gao, Xiaomin; Zhang, Chen; Hu, Cai; Zuo, Qishenng; Chen, Guohong; Li, Bichun

doi:10.1016/j.gene.2021.145760

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…Therefore, nowadays, avian germ cells are thought to be specified by the preformation model; however, the possibility that avian PGCs are formed via epigenetic regulation cannot be excluded [ 30 ]. Thus, while several studies have supported the preformation model in avian PGC formation, the origin of PGCs remains unknown.…”

Section: Early Development Of Chicken Pgcsmentioning

confidence: 99%

“…In chickens, BMP4 is also essential for the integrity of PGCs. While the nucleotide sequence in the promoter region of chicken BMP4 is poorly conserved with mammalian Bmp4 , the protein sequences in the coding region of BMP4 are highly conserved in birds and mammals [ 30 ]. Using chicken embryonic stem cells (ESCs) [ 68 , 69 ], Zuo et al demonstrated that BMP4 contributes to the induction of PGC formation from the ESCs [ 70 , 71 , 72 ].…”

Section: Integrity Of Chicken Pgcsmentioning

confidence: 99%

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Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms

Ichikawa

Horiuchi

2023

Genes

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Primordial germ cells (PGCs) are precursor cells of sperm and eggs. The fate decisions of chicken PGCs in terms of their development, integrity, and sex determination have unique features, thereby providing insights into evolutionary developmental biology. Additionally, fate decisions in the context of a self-renewal mechanism have been applied to establish culture protocols for chicken PGCs, enabling the production of genome-edited chickens and the conservation of genetic resources. Thus, studies on the fate decisions of chicken PGCs have significantly contributed to both academic and industrial development. Furthermore, studies on fate decisions have rapidly advanced owing to the recent development of essential research technologies, such as genome editing and RNA sequencing. Here, we reviewed the status of fate decisions of chicken PGCs and provided insight into other important research issues that require attention.

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Section: Early Development Of Chicken Pgcsmentioning

confidence: 99%

Section: Integrity Of Chicken Pgcsmentioning

confidence: 99%

Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms

Ichikawa

Horiuchi

2023

Genes

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“…DNA methylation is a form of epigenetics that plays an important role in cell differentiation, development, aging, and disease by regulating gene expression. DNA methylation has been widely studied in chickens, mainly involving embryonic development [ 12 ], meat quality traits [ 13 , 14 , 15 ], fat deposition [ 16 , 17 , 18 ], environmental adaptation [ 19 , 20 ], nutritional regulation [ 21 , 22 ], disease resistance [ 23 , 24 , 25 ], and reproductive traits [ 26 , 27 , 28 ]. However, these complex biological phenomena also occur during FM.…”

Section: Introductionmentioning

confidence: 99%

Chicken Hypothalamic and Ovarian DNA Methylome Alteration in Response to Forced Molting

Zhang

Li²,

Deng

et al. 2023

Animals

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Epigenetic modifications play an important role in regulating animal adaptation to external stress. To explore how DNA methylation regulates the expression levels of related genes during forced molting (FM) of laying hens, the hypothalamus and ovary tissues were analyzed at five periods using Whole-Genome Bisulfite Sequencing. The results show that methylation levels fluctuated differently in the exon, intron, 5′UTR, 3′UTR, promoter, and intergenic regions of the genome during FM. In addition, 16 differentially methylated genes (DMGs) regulating cell aging, immunity, and development were identified in the two reversible processes of starvation and redevelopment during FM. Comparing DMGs with differentially expressed genes (DEGs) obtained in the same periods, five hypermethylated DMGs (DSTYK, NKTR, SMOC1, SCAMP3, and ATOH8) that inhibited the expression of DEGs were found. Therefore, DMGs epigenetically modify the DEGs during the FM process of chickens, leading to the rapid closure and restart of their reproductive function and a re-increase in the egg-laying rate. Therefore, this study further confirmed that epigenetic modifications could regulate gene expression during FM and provides theoretical support for the subsequent optimization of FM technology.

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Selection of reference genes for RT-qPCR analysis in developing chicken embryonic ovary

Wang

Zhang

et al. 2023

Mol Biol Rep

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Epigenetic modification cooperates with Zeb1 transcription factor to regulate Bmp4 to promote chicken PGCs formation

Cited by 8 publications

References 26 publications

Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms

Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms

Chicken Hypothalamic and Ovarian DNA Methylome Alteration in Response to Forced Molting

Selection of reference genes for RT-qPCR analysis in developing chicken embryonic ovary

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