2007
DOI: 10.1093/nar/gkm118
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Epigenetic disruption of ribosomal RNA genes and nucleolar architecture in DNA methyltransferase 1 (Dnmt1) deficient cells

Abstract: The nucleolus is the site of ribosome synthesis in the nucleus, whose integrity is essential. Epigenetic mechanisms are thought to regulate the activity of the ribosomal RNA (rRNA) gene copies, which are part of the nucleolus. Here we show that human cells lacking DNA methyltransferase 1 (Dnmt1), but not Dnmt33b, have a loss of DNA methylation and an increase in the acetylation level of lysine 16 histone H4 at the rRNA genes. Interestingly, we observed that SirT1, a NAD+-dependent histone deacetylase with a pr… Show more

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Cited by 128 publications
(115 citation statements)
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“…Anti-Gal4 and anti-␤-actin immunoblot assays were performed to assess Gal4-DNMT1 expression and to serve as loading controls. and its consequent recruitment to rRNA genes in colon cancer cells (20). SIRT1, however, is not the only DNMT1 deacetylase.…”
Section: Discussionmentioning
confidence: 95%
“…Anti-Gal4 and anti-␤-actin immunoblot assays were performed to assess Gal4-DNMT1 expression and to serve as loading controls. and its consequent recruitment to rRNA genes in colon cancer cells (20). SIRT1, however, is not the only DNMT1 deacetylase.…”
Section: Discussionmentioning
confidence: 95%
“…For instance, in a-thalassemia/mental retardation syndrome X-linked (ATRX), hypomethylation of the ribosomal genes at chromosomes 13, 14, 15, 21, and 22 is known to be associated with mutations in ATRX, a chromatin remodeling factor (Gibbons et al 2000). Also, genetic manipulation of DNMT levels reduces the DNA methylation levels of ribosomal genes (Espada et al 2007). We therefore performed bisulfite genomic sequencing for the five original pairs of MZ twins discordant for SLE on three regions of the ribosomal RNA gene cluster: the ribosomal gene promoter, 18S, and 28S.…”
Section: Sle Discordant Mz Twins Are Differentially Methylated At Ribmentioning
confidence: 99%
“…Studies have shown that SirT1 colocalizes in rDNA regions with the DNA methyltransferase Dnmt1 (Espada et al 2007). Although the presence of SirT1 in these regions is dependent on Dnmt1, the levels of DNA methylation are unaffected by its absence.…”
Section: The Exertion Of Sirtuinsmentioning
confidence: 99%