Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation

Moraleda-Prados, Javier; Caballero‐Huertas, Marta; Valdivieso, A.; Joly, Sílvia; Ji, Jeong‐Seon; Roher, Nerea; Ribas, Laia

doi:10.1016/j.dci.2020.103848

Cited by 15 publications

(9 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another environmental factor, the density—the number of fishes in a tank—, caused masculinization in zebrafish [ 97 ] revealing some epigenetic alterations in the fish gonads [ 98 ]. Additionally, environmental-epigenetics interactions after immune stimulation during sex differentiation in zebrafish showed an alteration of the methylation of some immune-related genes [ 99 , 100 ]. The present data indicated that the epigenetic machinery was reprogrammed by gravity alterations, and although much data needs to be performed, particularly whether zebrafish would be used as a space-related animal model, the underlying epigenetic alterations need to be considered to fully comprehend the gravity-epigenome interactions.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Gravity is one of the most constant environmental factors across Earth’s evolution and all organisms are adapted to it. Consequently, spatial exploration has captured the interest in studying the biological changes that physiological alterations are caused by gravity. In the last two decades, epigenetics has explained how environmental cues can alter gene functions in organisms. Although many studies addressed gravity, the underlying biological and molecular mechanisms that occur in altered gravity for those epigenetics-related mechanisms, are mostly inexistent. The present study addressed the effects of hypergravity on development, behavior, gene expression, and most importantly, on the epigenetic changes in a worldwide animal model, the zebrafish (Danio rerio). To perform hypergravity experiments, a custom-centrifuge simulating the large diameter centrifuge (100 rpm ~ 3 g) was designed and zebrafish embryos were exposed during 5 days post fertilization (dpf). Results showed a significant decrease in survival at 2 dpf but no significance in the hatching rate. Physiological and morphological alterations including fish position, movement frequency, and swimming behavior showed significant changes due to hypergravity. Epigenetic studies showed significant hypermethylation of the genome of the zebrafish larvae subjected to 5 days of hypergravity. Downregulation of the gene expression of three epigenetic-related genes (dnmt1, dnmt3, and tet1), although not significant, was further observed. Taken altogether, gravity alterations affected biological responses including epigenetics in fish, providing a valuable roadmap of the putative hazards of living beyond Earth.

show abstract

Section: Discussionmentioning

confidence: 99%

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another environmental factors, the density —the number of fishes in a tank—, caused masculinization in zebrafish (Ribas, Valdivieso, et al, 2017) revealing some epigenetic alterations in the fish gonads (Caballero, unpublished results). Additionally, environmental-epigenetics interactions after immune stimulation during sex differentiation in zebrafish showed and alteration of the methylation of some immune-related genes (Caballero-Huertas et al, 2020; Moraleda-Prados et al, 2021). The present data indicated that the epigenetic machinery was reprogrammed by gravity alterations, and although many data needs to be performed, in particularly whether zebrafish would be used as a space-related animal model, the underlying epigenetic alterations need to be considered to fully comprehend the gravity-epigenome interactions.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Gravity is one of the most constant environmental factors across Earth’s evolution and all organisms are adapted to it. Consequently, spatial exploration has captured the interest in studying the biological changes that physiological alterations are caused by gravity. In the last two decades, epigenetics has explained how the environmental cues are able to altered gene functions in the organisms. Although many studies addressed gravity, the underlying biological and molecular mechanisms that are occurred in altered gravity for those epigenetics-related mechanisms, are mostly inexistent. The present study addressed the effects of hypergravity on development, behavior, gene expression, and most importantly, on the epigenetic changes in a world-wide animal model, the zebrafish (Danio rerio). To perform hypergravity experiments, a custom-centrifuge simulating the large diameter centrifuge (100 rpm ∼ 3g) were designed and zebrafish embryos were exposed during 5 days post fertilization (dpf). Results showed a significant decrease of survival at 2 dpf but not significance in the hatching rate. Physiological and morphological alterations including fish position, movement frequency and swimming behavior showed significant changes due to hypergravity. Epigenetic studies showed a significant hypermethylation of the genome of the zebrafish larvae subjected to 5 days of hypergravity. A downregulation of the gene expression of three epigenetic-related genes (dnmt1,dnmt3, andtet1), although not significant, were further observed. Taken altogether, gravity alterations affected biological responses including epigenetics in fish, providing a valuable roadmap of the putative hazards of living beyond Earth.

show abstract

“…In fact, head kidney is considered the principal immune organ in fish (Rauta et al 2012) and it is developed from larval pronephros, where also P. neurophilia presence has been recorded (Figure 2a) (Sanders et al 2014). Moreover, pronephros has been recently described as a location where lipopolysaccharide (LPS) is recruited after bath immune stimulation in zebrafish larvae (Moraleda-Prados et al, 2021). Therefore, future studies on both head kidney and pronephros roles in P. neurophilia infections are of interest to cope with the disease by developing immunity.…”

Section: Target Organs For Pseudoloma Neurophilia Infestationmentioning

confidence: 99%

“…2014). Moreover, pronephros has been recently described as a location where lipopolysaccharide (LPS) is recruited after bath immune stimulation in zebrafish larvae (Moraleda‐Prados et al, 2021). Therefore, future studies on both head kidney and pronephros roles in P. neurophilia infections are of interest to cope with the disease by developing immunity.…”

Section: Target Organs For Pseudoloma Neurophilia Infestationmentioning

confidence: 99%

“…different LPS strains) feminized zebrafish populations in dose and LPS‐strain‐dependent manner (Moraleda‐Prados et al . 2021). Therefore, and due to the plasticity of the sexual phenotype in early stages, it would not be surprising that the sex ratio of zebrafish populations was altered by the effects of pathogen colonization, including microsporidia.…”

Section: Alterations In the Reproductive Systemmentioning

confidence: 99%

See 1 more Smart Citation

Reviewing Pseudoloma neurophilia infections in the popular zebrafish model

Caballero‐Huertas

Soto

Ribas

2021

Reviews in Aquaculture

Self Cite

View full text Add to dashboard Cite

Zebrafish is a worldwide used animal model to better understand cultured fish species; thus, in the last two decades, zebrafish facilities have been created in many aquaculture research centres. However, and despite the attempts of rigorous aseptic conditions for animal husbandry in wet laboratory facilities, the presence of parasites cannot be underestimated. The microsporidium Pseudoloma neurophilia, which mostly affects the central nervous system, has been listed as the most prevalent parasite in reared zebrafish. The emergence of P. neurophilia is usually detected when the individual presents irreversible morphological and/or behavioural symptoms, and consequently, the spread of the disease is inevitable, indicating that the infection is expanded across rearing tanks. This review attempts, for the first time, to collect and discuss the current information on P. neurophilia life cycle, target tissues and symptomatology of the infection occurred in zebrafish. Due to its vertical transmission together with the increasing presence of zebrafish as a model in reproduction-related problems found in aquaculture, it is highlighted, among others, the sexual dimorphism of the pathogenic effects and the possible transgenerational implications of parasitic infections. Further, to date none effective treatments have been described, so here we provide in detail available diagnostic methods, discussing the importance of the early detection, giving safety-rearing recommendations to decrease the presence of P. neurophilia. Our intention is to minimize parasite outbreaks in zebrafish facilities, which, on the one hand, can compromise zebrafish welfare, and on the other hand, could interfere furtively in the resulting research previously designed for improving aquaculture production but also other biomedical-related research.

show abstract

Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation

Cited by 15 publications

References 82 publications

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Reviewing Pseudoloma neurophilia infections in the popular zebrafish model

Contact Info

Product

Resources

About