Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen

Chakraborty, Joydeep; Ghosh, Prithwi; Sen, Senjuti; Das, Sampa

doi:10.1016/j.plantsci.2018.07.014

Cited by 32 publications

(21 citation statements)

References 79 publications

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“…The WRKY40 protein has been shown to be involved in the ABA signaling response (Chen et al ., ; Liu et al ., ), defense against pathogens (Xu et al ., ; Pandey et al ., ; Abeysinghe et al ., ; Chakraborty et al ., ; Wang C. et al ., ) and salt tolerance (Dai et al ., ). The role of WRKY40 in response to wounding and in the regulation of anthocyanin biosynthesis might enrich functional studies of the WRKY40 protein.…”

Section: Discussionmentioning

confidence: 99%

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

et al. 2019

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Summary Wounding stress leads to anthocyanin accumulation. However, the underlying molecular mechanism remains elusive. In this study, MdWRKY40 was found to promote wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergo MdBT2‐mediated degradation in apple. We found that MdMYB1, a positive regulator of anthocyanin biosynthesis, was essential for the wounding‐induced anthocyanin biosynthesis in apple. MdWRKY40 was identified as an MdMYB1‐interacting protein, and enhanced the binding of MdMYB1 to its target genes in response to wounding. We found that MdBT2 interacted physically with MdWRKY40 and was involved in its degradation through the 26S proteasome pathway. Our results demonstrate that MdWRKY40 is a key modulator in the wounding‐induced anthocyanin biosynthesis, which provides new insights into the regulation of wounding‐induced anthocyanin biosynthesis at both the transcriptional and post‐translational levels in apple.

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Section: Discussionmentioning

confidence: 99%

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

et al. 2019

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“…In addition, WRKY transcription factors can combine with W box elements in their own gene promoters. In chickpea infected with Fusarium , for example, WRKY40 has been found to bind to its own promoter to regulate its gene expression [40]. Many genes in the three Asterales species examined in this study contain W box elements.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of WRKY Transcription Factors in the Asteranae

Guo

Zhang

Wang

et al. 2019

Plants

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The WRKY transcription factors family, which participates in many physiological processes in plants, constitutes one of the largest transcription factor families. The Asterales and the Apiales are two orders of flowering plants in the superorder Asteranae. Among the members of the Asterales, globe artichoke (Cynara cardunculus var. scolymus L.), sunflower (Helianthus annuus L.), and lettuce (Lactuca sativa L.) are important economic crops worldwide. Within the Apiales, ginseng (Panax ginseng C. A. Meyer) and Panax notoginseng (Burk.) F.H. Chen are important medicinal plants, while carrot (Daucus carota subsp. carota L.) has significant economic value. Research involving genome-wide identification of WRKY transcription factors in the Asterales and the Apiales has been limited. In this study, 490 WRKY genes, 244 from three species of the Apiales and 246 from three species of the Asterales, were identified and categorized into three groups. Within each group, WRKY motif characteristics and gene structures were similar. WRKY gene promoter sequences contained light responsive elements, core regulatory elements, and 12 abiotic stress cis-acting elements. WRKY genes were evenly distributed on each chromosome. Evidence of segmental and tandem duplication events was found in all six species in the Asterales and the Apiales, with segmental duplication inferred to play a major role in WRKY gene evolution. Among the six species, we uncovered 54 syntenic gene pairs between globe artichoke and lettuce. The six species are thus relatively closely related, consistent with their traditional taxonomic placement in the Asterales. This study, based on traditional species classifications, was the first to identify WRKY transcription factors in six species from the Asteranae. Our results lay a foundation for further understanding of the role of WRKY transcription factors in species evolution and functional differentiation.

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“…10 a). Previous studies showed that stress-induced genes that are upregulated following cold treatment were associated with the histone enrichment of H3K9 acetylation and H3K4 methylation (H3K9ac/H3K4me3), which are known as activating epigenetic marks, in the promoter region [ 21 – 23 ]. To investigate whether the histone modifications influenced the gene expression epigenetically between these two geographically distinct populations, we measured the levels of H3K9ac and H3K4me3 at the WRKY40 gene locus before and after cold stress.…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis reveals ecological adaption of cold tolerance in northward invasion of Alternanthera philoxeroides

et al. 2020

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Background Alternanthera philoxeroides (alligator weed) is a highly invasive alien plant that has continuously and successfully expanded from the tropical to the temperate regions of China via asexual reproduction. During this process, the continuous decrease in temperature has been a key limiting environmental factor. Results In this study, we provide a comprehensive analysis of the cold tolerance of alligator weed via transcriptomics. The transcriptomic differences between the southernmost population and the northernmost population of China were compared at different time points of cold treatments. GO enrichment and KEGG pathway analyses showed that the alligator weed transcriptional response to cold stress is associated with genes encoding protein kinases, transcription factors, plant-pathogen interactions, plant hormone signal transduction and metabolic processes. Although members of the same gene family were often expressed in both populations, the levels of gene expression between them varied. Further ChIP experiments indicated that histone epigenetic modification changes at the candidate transcription factor gene loci are accompanied by differences in gene expression in response to cold, without variation in the coding sequences of these genes in these two populations. These results suggest that histone changes may contribute to the cold-responsive gene expression divergence between these two populations to provide the most beneficial response to chilling stimuli. Conclusion We demonstrated that the major alterations in gene expression levels belonging to the main cold-resistance response processes may be responsible for the divergence in the cold resistance of these two populations. During this process, histone modifications in cold-responsive genes have the potential to drive the major alterations in cold adaption necessary for the northward expansion of alligator weed.

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Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen

Cited by 32 publications

References 79 publications

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

Genome-Wide Identification of WRKY Transcription Factors in the Asteranae

Comparative transcriptome analysis reveals ecological adaption of cold tolerance in northward invasion of Alternanthera philoxeroides

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