Epigenetic and hormonal profile during maturation of Quercus Suber L. somatic embryos

Pérez, Marta; Viejo, Marcos; Lacuesta, M.; Toorop, Peter E.; Cañal, María Jesús

doi:10.1016/j.jplph.2014.07.028

Cited by 30 publications

(28 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Huang et al (2012) highlighted that endogenous auxin stimulate shoot regeneration in rice calli. Abscisic acid (ABA) supplemented medium produces high quality somatic embryos by decreased osmotic water potential compared to the maintenance medium (Klimaszewska et al, 2000;Pérez et al, 2015). Osmotic water potential depends on types of media, carbon sources and gelleting agent concentrations (Hadeler et al, 1995;Klimaszewska et al, 1997;Laine et al, 2000;Triqui et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Rahman

Ramli

Kamaruzaman

et al. 2015

Emir. J. Food Agric

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Rahman

Ramli

Kamaruzaman

et al. 2015

Emir. J. Food Agric

View full text Add to dashboard Cite

“…Those authors showed that the observed changes could be related to the toxicity of DMSO as a component of PVS2. DNA methylation plays a key role in the control of plant development (Perez et al 2015); thus, in our study, the expression of genes responsible for the rooting of seedlings may have started after 30 days of post-thaw culture. The stronger development of the roots of F. sylvatica was likely a result of major changes in DNA methylation in hypocotyls.…”

Section: Dna Methylation During In Vitro Culturementioning

confidence: 76%

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Nuc

Marszałek

Pukacki

2016

Trees

View full text Add to dashboard Cite

Key message Dehydration and cryo-stress (2196°C) caused a greater increase in the DNA methylation of embryonic axes (EAs) of beech seeds (tolerant to both stresses) than in EAs of oak (sensitive to both stresses). Simultaneously, in vitro survival of cryopreserved beech EAs was also significantly higher than in those of oak. Abstract DNA methylation plays a key role in the regulation of growth and differentiation in plants. The present study focused on global DNA methylation of embryonic axes (EAs) isolated from the seeds of pedunculate oak (Quercus robur L.) and European beech (Fagus sylvatica L.) after cryopreservation. Oak seeds can be labeled recalcitrant, i.e., sensitive to dehydration, while beech seeds fall under the suborthodox category, i.e., tolerant to dehydration. DNA methylation was determined using a specific antibody directed at the methylated cytosine in CpG dinucleotides. The analysis was carried out on DNA isolated from EA tissues and subjected to (1) dehydration (control), (2) a combination of dehydration and plant vitrification solution (PVS3) and (3) dehydration with vitrification PVS3, and rapid cooling in liquid nitrogen (LN 2 , -196°C) at a cooling rate of 32°C s -1 . Such treatments induced global DNA methylation primarily in tolerant EAs from F. sylvatica seeds. The level of global DNA methylation in this material was 30 % higher than in the control, i.e., dehydrated (not frozen) beech EAs. The same treatment in oak EAs did not evoke such changes. After 120 days of tissue growth, we observed substantially more DNA demethylation in the beech tissues after cryopreservation in LN 2 than in the controls or in tissues subjected to vitrification but not cryopreservation. Information on the global level of DNA methylation can be useful in monitoring procedures aimed at increasing the rate of recovery of EAs following cryopreservation.

show abstract

“…Somatic embryogenesis procedure was accomplished following previous reports (Bueno et al 2000;Pérez et al 2015). Embryogenic cultures were initiated from a pool of immature embryos of Q. suber L. collected during the period Plant Cell Tiss Organ Cult of fruit development according to Bueno et al (2000).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Once somatic embryogenesis was inducted, embryogenic lines were transferred to a medium supplemented with 3.4 mM glutamine and maintained in a proliferating phase by secondary embryogenesis and monthly subculture. Before each subculture, somatic embryos at a cotyledonary stage and without signs of recurrent embryogenesis were matured according to Pérez et al (2015). Somatic embryos were cultured on a medium supplemented with 1 % activated charcoal for 1 month in darkness at 25°C followed by 60 days in darkness at 4°C.…”

Section: Plant Materialsmentioning

confidence: 99%

“…During somatic embryogenesis, embryo development and maturation are regulated by a complex signalling network that integrates genetic and epigenetic programs as well as hormonal and metabolic signals (Gutierrez et al 2007;Gao et al 2012). Abscisic acid (ABA) plays a key role in many developmental processes and more specifically endogenous ABA levels are correlated with the correct development and maturation of seeds and somatic embryos (Rai et al 2011;Pérez et al 2015). ABA forms a master regulator of the synthesis and deposition of storage proteins during somatic embryogenesis, as well as prevents precocious germination in maturing embryos.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression analysis of epigenetic and abscisic acid-related genes during maturation of Quercus suber somatic embryos

Pérez

Cañal

Toorop

2015

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

Clonal propagation of Quercus suber via somatic embryogenesis is an alternative to conventional tree propagation methods; however, complete maturation of somatic embryos is considered the major bottleneck for mass propagation of Quercus species. During somatic embryogenesis, embryo development and maturation are controlled by signaling pathways that integrate information from genetic and epigenetic programs as well as hormonal signals. Therefore, in this study genes were identified related to epigenetic regulation and the abscisic acid (ABA) pathway during development and maturation of cork oak somatic embryos. A total of eight expressed sequence tags were obtained of genes encoding a 9-cisepoxycarotenoid dioxygenase (NCED), two histone deacetylases (HDA6 and HDA19), two histone monoubiquitinases (HUB1 and HUB2), a histone H3 kinase (AUR3) as well as genes related to chromatin remodeling processes PICKLE and VP1/ABSCISIC ACID INSENSI-TIVE 3-LIKE 1 (VAL1). The analysis of the expression patterns of selected genes during different developmental stages indicated that QsNCED3 may play a role in ABA synthesis during embryogenesis. The change in the expression levels for all seven genes associated with epigenetic regulation showed that QsHUB1 and QsHUB2 may have a role in ABA signalling while QsHDA6 and QsHDA19 could act in different pathways than in Arabidopsis. Furthermore, expression levels of QsAUR3 indicated that histone phosphorylation is an early epigenetic mark in Q. suber somatic embryos while QsPICKLE and QsVAL1 may be necessary for the correct development of cork oak somatic embryos.

show abstract

Epigenetic and hormonal profile during maturation of Quercus Suber L. somatic embryos

Cited by 30 publications

References 51 publications

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Expression analysis of epigenetic and abscisic acid-related genes during maturation of Quercus suber somatic embryos

Contact Info

Product

Resources

About