Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: Potential for HIV-1 therapy

Williamson, Michael P.; McCormick, Theron G.; Nance, Christina; Shearer, William T.

doi:10.1016/j.jaci.2006.08.016

Cited by 181 publications

(118 citation statements)

References 32 publications

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“…S-11). In conclusion, these and previously published results point to EGCG as a generic binder [34][35][36][37][38][39][40][41][42] and modulator 41,42 of protein structure.…”

Section: Egcg Binding Promotes As Compactionsupporting

confidence: 79%

Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein

et al. 2016

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The intrinsically disordered and amyloidogenic protein -synuclein (AS) has been linked to several neurodegenerative states, including Parkinson's disease. Here, nano-electrospray-ionization mass spectrometry (nano-ESI-MS), ion mobility (IM), and native top-down electron transfer dissociation (ETD) techniques are employed to study AS interaction with small molecules known to modulate its aggregation, such as epigallocatechin-3-gallate (EGCG) and dopamine (DA). The complexes formed by the two ligands under identical conditions reveal peculiar differences. While EGCG engages AS in compact conformations, DA preferentially binds to the protein in partially extended conformations. The two ligands also have different effects on AS structure as assessed by IM, with EGCG leading to protein compaction and DA to its extension. Native top-down ETD on the protein-ligand complexes shows how the different observed modes of binding of the two ligands could be related to their known opposite effects on AS aggregation. The results also show that the protein can bind either ligand in the absence of any covalent modifications, such as e.g. oxidation.

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“…S-11). In conclusion, these and previously published results point to EGCG as a generic binder [34][35][36][37][38][39][40][41][42] and modulator 41,42 of protein structure.…”

Section: Egcg Binding Promotes As Compactionsupporting

confidence: 79%

Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein

et al. 2016

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“…However, given the components in the mixture, it is likely to be effective in acute infection. in this respect, it should be noted that micronutrients such as ascorbate (vitamin c) have also been shown to suppress HiV replication in acutely infected cells (9), and epigallocatechin gallate (eGcG), a component of GT, has been reported to inhibit HiV binding to host cells (11,21). This broad-based anti-HIV action of specific micronutrients at various phases of viral infection combined with their minimal cellular toxicity suggests the potential value of these natural compounds in controlling HiV infection.…”

Section: Discussionmentioning

confidence: 99%

“…eGcG has been shown to have anti-HiV-1 activity in human lymphocytes in vitro by inhibiting the biochemical activity of HiV-1 rT, resulting in a decrease in HiV-1 p24 antigen concentration (25). it has also been shown to prevent the binding of HiV-1 glycoprotein 120 to the cd4 molecule on T cells (11,21).…”

Section: Discussionmentioning

confidence: 99%

Micronutrient cooperation in the suppression of HIV production in chronically and latently infected cells

Jariwalla

Gangapurkar²,

Pandit³

et al. 2010

Mol Med Rep

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Abstract. nutrients are known to display pharmacologic activity against viruses and to exert cooperative effects in cells. To study the influence of nutrient cooperation on HiV production in chronically infected T lymphocytes, we evaluated the individual and combined effects of nutrients on HiV-1 reverse transcriptase (rT) released into the culture supernatant. in unstimulated cells, low concentrations of single nutrients, namely ascorbic acid (aa), green tea polyphenols (GT) or lysine, did not significantly suppress HiV-1 rT production. However, when GT (25 µg/ml) and aa (32-64 µg/ml) were combined and applied to cells, extracellular RT was significantly reduced relative to the control. combining GT (25 µg/ml) with lysine (25 µg/ml) also reduced the rT level to a greater extent (51% of control) than was observed wih lysine alone, and the addition of aa (16 µg/ml) to the combination further decreased rT to 17% of the control (p=0.06). under the same assay conditions, the nucleoside analog azidothymidine did not significantly suppress HiV production at low to moderate concentrations (0.5-1.0 µg/ml), but did reduce the rT level to 40% of the control (p=0.02) at the highest dose tested (2 µg/ml). in unstimulated cells as well as in latently infected cells stimulated with mitogen (PMa or TnF-α), a nutrient mixture containing GT, AA and amino acids imparted significantly greater rT suppression than equivalent concentrations of key individual components. nutrient effects on rT suppression were virus-specific and were not due to non-specific cellular toxicity. These results suggest that relatively non-toxic micronutrient combinations are more potent than single nutrients in suppressing virus production in chronically infected T cells, indicating that the constituent nutrients have a cooperative effect in HiV inhibition.

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“…EGCG has been reported to possess several biochemical and pharmacological properties, which include anti-HIV and HCV infection activity (49,94,318,325), reduction of Sjögren's syndrome in murine models (83,110), prevention of Alzheimer's and Parkinson's diseases (203), anti-obesity effects on mice and humans (41,319), anti-oxidant activity (75,104,293,348), and anti-neoplastic activity (135,262). The cancer-preventive effects of EGCG have been proposed to suppress the transformative, hyperproliferative, and inflammatory processes that are involved in carcinogenesis (288).…”

Section: The Inhibitory Mechanisms Of Natural Compounds Against Npc Smentioning

confidence: 99%

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

Chen¹

2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: Potential for HIV-1 therapy

Cited by 181 publications

References 32 publications

Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein

Opposite Structural Effects of Epigallocatechin-3-gallate and Dopamine Binding to α-Synuclein

Micronutrient cooperation in the suppression of HIV production in chronically and latently infected cells

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

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