Epigallocatechin gallate inhibits intracellular survival of Listeria monocytogenes in macrophages

Kohda, Chikara; Yanagawa, Yoko; Shimamura, Tadakatsu

doi:10.1016/j.bbrc.2007.10.190

Cited by 41 publications

(28 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kohda et al recently showed that sub-MIC levels of epigallocatechin gallate inhibit the growth of L. monocytogenes within macrophages [123]. This was attributed to LLO inhibition following observation that the flavan-3-ol prevented LLO from binding to membrane lipid and inhibited LLO-induced lysis [123]. If this is the case, then LLO inhibition may also be partially responsible for the activity recently detected from ␤-naphthoflavone against L. monocytogenes in hepatocytes [124].…”

Section: Inhibition Of Listeriolysin O (Llo)mentioning

confidence: 92%

“…Secretion of this protein enables bacteria to escape from phagosomes and enter the cytosol of host cells where they can multiply [122]. Kohda et al recently showed that sub-MIC levels of epigallocatechin gallate inhibit the growth of L. monocytogenes within macrophages [123]. This was attributed to LLO inhibition following observation that the flavan-3-ol prevented LLO from binding to membrane lipid and inhibited LLO-induced lysis [123].…”

Section: Inhibition Of Listeriolysin O (Llo)mentioning

confidence: 95%

See 1 more Smart Citation

Recent advances in understanding the antibacterial properties of flavonoids

Cushnie

Lamb

2011

International Journal of Antimicrobial Agents

1,002

672

View full text Add to dashboard Cite

Antibiotic resistance is a major global problem and there is a pressing need to develop new therapeutic agents. Flavonoids are a family of plant-derived compounds with potentially exploitable activities, including direct antibacterial activity, synergism with antibiotics, and suppression of bacterial virulence. In this review, recent advances towards understanding these properties are described. Information is presented on the ten most potently antibacterial flavonoids as well as the five most synergistic flavonoid-antibiotic combinations tested in the last 6 years (identified from PubMed and ScienceDirect). Top of these respective lists are panduratin A, with minimum inhibitory concentrations (MICs) of 0.06-2.0 μg/mL against Staphylococcus aureus, and epicatechin gallate, which reduces oxacillin MICs as much as 512-fold. Research seeking to improve such activity and understand structure-activity relationships is discussed. Proposed mechanisms of action are also discussed. In addition to direct and synergistic activities, flavonoids inhibit a number of bacterial virulence factors, including quorum-sensing signal receptors, enzymes and toxins. Evidence of these molecular effects at the cellular level include in vitro inhibition of biofilm formation, inhibition of bacterial attachment to host ligands, and neutralisation of toxicity towards cultured human cells. In vivo evidence of disruption of bacterial pathogenesis includes demonstrated efficacy against Helicobacter pylori infection and S. aureus α-toxin intoxication.

show abstract

Section: Inhibition Of Listeriolysin O (Llo)mentioning

confidence: 92%

Section: Inhibition Of Listeriolysin O (Llo)mentioning

confidence: 95%

Recent advances in understanding the antibacterial properties of flavonoids

Cushnie

Lamb

2011

International Journal of Antimicrobial Agents

1,002

672

View full text Add to dashboard Cite

show abstract

“…They have also reported that EGCG, inhibits InhA, the enoyl-ACP reductase of MT and interferes with the binding of NADH to InhA. Kohda et al have reported significant antimicrobial activity of ECGC against Listeria monocytogenes, another intracellular bacterium like MT [10] . Hu et al have observed a potent synergy between EGCG and Ampicillin/Sulbactam in the treatment of Methicillinresistant Staphylococcus aureus infection [11] .…”

Section: Research Papermentioning

confidence: 99%

Epigallocatechin Gallate, a Green Tea Polyphenol Inhibits Mycobacterium smegmatis: In silico and In vitro Studies

Narayanan¹,

Ramesh²

2017

pharmaceutical-sciences

View full text Add to dashboard Cite

Narayanan and Ramesh: Epigallocatechin Gallate Inhibits M. smegmatisPresence of multidrug resistant strains makes treatment of tuberculosis difficult. Conventional frontline antituberculosis therapy has high incidence of treatment failure due to non-adherence and severe side effects, and therefore warrants alternative approaches using safe, natural compounds. (-) Epigallocatechin gallate, a green tea catechin with significant antimicrobial properties, and its derivatives have been studied for their inhibitory effects on Mycobacterium smegmatis. Enoyl reductase (Inh A, PDB ID: 2NV6), an important drug target inhibited by the antituberculosis drug, isoniazid, was docked with geometrically optimized conformation of epigallocatechin gallate and two of its derivatives using AutoDock software. Perpropionate and permethyl derivatives of epigallocatechin gallate were synthesized and, along with epigallocatechin gallate, evaluated for their inhibitory and cytotoxic properties against M. smegmatis using microplate dilution method and alamarBlue® assay, respectively. Ames test was performed to assess the mutagenic potential of epigallocatechin gallate. It was docked successfully onto the InhA with docking energy of -9.38 kcal mol -1 . Analysis of neighbouring groups revealed 11 amino acid residues common to both isoniazid and epigallocatechin gallate. The minimum inhibitory concentrations for epigallocatechin gallate, perpropionate and permethyl were found to be 128 (58.2% inhibition), 8 (32.9%) and 4 (12.5%) µg/ml, respectively. Epigallocatechin gallate showed a cytotoxicity of 18.6% at a concentration of 8 µg/ml, while the two derivatives did not show any toxicity, even at 64 µg/ml. Ames test revealed the absence of mutagenicity for epigallocatechin gallate, in presence and absence of metabolic activation. Nonmutagenic epigallocatechin gallate inhibits the growth of M. smegmatis and warrants further investigation as an adjunct therapy for pathogenic mycobacteria.

show abstract

“…EGCG was shown to inhibit the growth of Bacillus subtilis through binding to several surface membrane proteins [111]. EGCG was also shown to inhibit Listeria monocytogenes growth in macrophages derived from murine peritoneal cavity, through mechanisms that involved inhibition of disruption of the phagosomal membrane, which is important during the escaping phase of the bacteria [112]. Although most of these studies have been performed in vitro , there is sufficient evidence to evoke interest in development of select classes of flavonoids as alternatives to antiviral and antibiotics.…”

Section: 3 Antimicrobial Properties Of Flavonoidsmentioning

confidence: 99%

Induction of cellular and molecular immunomodulatory pathways by vitamin A and flavonoids

Patel¹,

Vajdy²

2015

Expert Opinion on Biological Therapy

View full text Add to dashboard Cite

Introduction A detailed study of reports on the immunomodulatory properties of vitamin A and select flavonoids may pave the way for using these natural compounds or compounds with similar structures in novel drug and vaccine designs against infectious and autoimmune diseases and cancers. Areas Covered Intracellular transduction pathways, cellular differentiation and functional immunomodulatory responses have been reviewed. The reported studies encompass in vitro, in vivo preclinical and clinical studies that address the role of Vitamin A and select flavonoids in induction of innate and adaptive B and T cell responses, including TH1, TH2 and Treg. Expert Opinion While the immunomodulatory role of vitamin A, and related compounds, is well-established in many preclinical studies, its role in humans has begun to gain wider acceptance. In contrast, the role of flavonoids is mostly controversial in clinical trials, due to the diversity of the various classes of these compounds, and possibly due to the purity and the selected doses of the compounds. However, current preclinical and clinical studies warrant further detailed studies of these promising immuno-modulatory compounds.

show abstract

Epigallocatechin gallate inhibits intracellular survival of Listeria monocytogenes in macrophages

Cited by 41 publications

References 29 publications

Recent advances in understanding the antibacterial properties of flavonoids

Recent advances in understanding the antibacterial properties of flavonoids

Epigallocatechin Gallate, a Green Tea Polyphenol Inhibits Mycobacterium smegmatis: In silico and In vitro Studies

Induction of cellular and molecular immunomodulatory pathways by vitamin A and flavonoids

Contact Info

Product

Resources

About