Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1/NF-κB pathway in mast cells

Moon, Phil‐Dong; Choi, In-Hwa; Kim, Hyung-Min

doi:10.1007/s00726-011-1050-8

Cited by 18 publications

(10 citation statements)

References 31 publications

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“…TSLP is a cytokine of the IL-7 family, is produced mainly by stromal cells, including mast cells,83–86 and is involved in the activation, expansion, and survival of T lymphocytes and dendritic cells. TSLP expression in the airway epithelium is inducible through a NF-κB-dependent pathway 87,88.…”

Section: Individual Cytokines Involved In the Pathogenesis Of Copdmentioning

confidence: 99%

Cytokine inhibition in the treatment of COPD

Caramori¹,

Adcock²,

Stefano³

et al. 2014

COPD

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Cytokines play an important part in many pathobiological processes of chronic obstructive pulmonary disease (COPD), including the chronic inflammatory process, emphysema, and altered innate immune response. Proinflammatory cytokines of potential importance include tumor necrosis factor (TNF)-α, interferon-γ, interleukin (IL)-1β, IL-6, IL-17, IL-18, IL-32, and thymic stromal lymphopoietin (TSLP), and growth factors such as transforming growth factor-β. The current objectives of COPD treatment are to reduce symptoms, and to prevent and reduce the number of exacerbations. While current treatments achieve these goals to a certain extent, preventing the decline in lung function is not currently achievable. In addition, reversal of corticosteroid insensitivity and control of the fibrotic process while reducing the emphysematous process could also be controlled by specific cytokines. The abnormal pathobiological process of COPD may contribute to these fundamental characteristics of COPD, and therefore targeting cytokines involved may be a fruitful endeavor. Although there has been much work that has implicated various cytokines as potentially playing an important role in COPD, there have been very few studies that have examined the effect of specific cytokine blockade in COPD. The two largest studies that have been reported in the literature involve the use of blocking antibody to TNFα and CXCL8 (IL-8), and neither has provided benefit. Blocking the actions of CXCL8 through its CXCR2 receptor blockade was not successful either. Studies of antibodies against IL-17, IL-18, IL-1β, and TSLP are currently either being undertaken or planned. There is a need to carefully phenotype COPD and discover good biomarkers of drug efficacy for each specific target. Specific groups of COPD patients should be targeted with specific anticytokine therapy if there is evidence of high expression of that cytokine and there are features of the clinical expression of COPD that will respond.

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Section: Individual Cytokines Involved In the Pathogenesis Of Copdmentioning

confidence: 99%

Cytokine inhibition in the treatment of COPD

Caramori¹,

Adcock²,

Stefano³

et al. 2014

COPD

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show abstract

“…We used the methods of Moon et al (2012) and Oh et al (2012), using an easy-BLUE™ RNA extraction kit (iNtRON Biotech, Republic of Korea) and isolated the total RNA from HMC-1 cells in accordance with the manufacturer's specification. The concentrations of total RNA in the final elutes were determined by spectrophotometer.…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Anamentioning

confidence: 99%

“…We used the enzyme-linked immunosorbent assay (ELISA) method to assay the culture supernatant for TSLP (Moon et al, 2012). A sandwich ELISA for TSLP was carried out in duplicate in a 96-well ELISA plate.…”

Section: Cytokine Assaymentioning

confidence: 99%

Effects of schizandrin on the expression of thymic stromal lymphopoietin in human mast cell line HMC-1

2012

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“…We used the enzyme-linked immunosorbent assay (ELISA) method to assay the culture supernatant for TSLP [22,24]. A sandwich ELISA for TSLP was carried out in duplicate in a 96-well ELISA plate.…”

Section: Cytokine Assaymentioning

confidence: 99%

“…We used the method of Moon et al [22], using an easy-BLUE™ RNA extraction kit (iNtRON Biotech, Republic of Korea) and isolated the total RNA from HMC-1 cells in accordance with the manufacturer's specifications. The concentrations of total RNA in the final elutes were determined by a spectrophotometer.…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Anamentioning

confidence: 99%

Down-regulation of thymic stromal lymphopoietin by curcumin

Moon

Jeong

Kim

2013

Pharmacological Reports

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Abstract:Background: Thymic stromal lymphopoietin (TSLP) is a cytokine implicated in the pathogenesis of allergic diseases such as asthma, atopic dermatitis and allergic rhinitis. Curcumin has various effects such as antidepressant, antioxidant, antihyperglycemic, antitumor and anti-inflammatory. However, the effect of curcumin on the production of TSLP has not been clarified. Thus, we investigated how curcumin inhibits the expression and production of TSLP in the human mast cell line, HMC-1 cells. Methods: We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and caspase-1 assay to investigate the effects of curcumin. Results:The results show that curcumin inhibited the production and mRNA expression of TSLP in HMC-1 cells: the maximal inhibition rate of TSLP production by curcumin (50 µM) was 59.16 ± 4.20%. In addition, curcumin suppressed the nuclear factor-kB luciferase activity induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, caspase-1 activity was increased, whereas caspase-1 activity was decreased by pretreatment with curcumin. Conclusion: These results suggest that curcumin can be used to treat inflammatory and atopic diseases through the suppression of TSLP.

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Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1/NF-κB pathway in mast cells

Cited by 18 publications

References 31 publications

Cytokine inhibition in the treatment of COPD

Cytokine inhibition in the treatment of COPD

Effects of schizandrin on the expression of thymic stromal lymphopoietin in human mast cell line HMC-1

Down-regulation of thymic stromal lymphopoietin by curcumin

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