Epigallocatechin-3-O-gallate Decreases Tumor Necrosis Factor-α–induced Fractalkine Expression in Endothelial Cells by Suppressing NF-ĸB

Abstract

Epigallocatechin-3-O-gallate (EGCG), the main catechin in green tea, has anti-oxidant, anti-atherosclerotic and anti-inflammatory properties. Fractalkine, a chemokine involved in inflammation and early atherosclerotic processes, acts as a chemoattractant as well as an adhesion molecule in endothelial cells activated by proinflammatory cytokines. In the present study, we investigated the effect of EGCG on fractalkine expression in TNF-α-induced human umbilical vein endothelial cells (HUVECs). EGCG decreased TNF-α-induced fractalkine mRNA and protein expression in HUVECs in a time-dependent manner. EGCG suppressed the TNF-α-induced phosphorylation and degradation of IκB-α, thereby decreasing the phosphorylation and nuclear translocation of the NF-κB p65 subunit in HUVECs. The DNA binding activity of the NF-κB p65 subunit was lower in EGCG-pretreated HUVECs than in those treated with TNF-α alone. Furthermore, EGCG inhibited monocyte adhesion to HUVECs stimulated by TNF-α. The silencing of fractalkine with an siRNA or treatment with a blocking antibody against fractalkine suppressed the TNF-α-induced increase in monocyte adhesion. These results demonstrate that EGCG prevents TNF-α-induced vascular endothelial fractalkine expression.

Green Tea Polyphenol Epigallocatechin-3-Gallate Inhibits TNF-a-Induced Production of Monocyte Chemoattractant Protein-1 in Human Umbilical Vein Endothelial Cells

“…In our study, the concentrations of EGCG (10-50μM) are generally within the range used in most published in vitro studies [27,28,29]. Nevertheless, the relatively high concentrations of EGCG obtained in cell culture studies have been considered to far exceed the levels found in human plasma after green tea consumption.…”

Genistein Suppression of TNF-α-induced Fractalkine Expression in Endothelial Cells

“…Our previous data showed that TNF-α-induced expression of fractalkine occurs mainly through the activation of the NF-κB and PI3-K pathways [28]. Recently, we reported that genistein protects against cisplatin-induced renal injury via the NF-κB signaling pathway [22].…”

Sulforaphane Inhibits TNF-α-Induced Adhesion Molecule Expression Through the Rho A/ROCK/NF-κB Signaling Pathway

“…EGCG decreased TNF-ainduced fractalkine expression by suppressing NF-jB 44 and inhibited TNF-a-induced MCP-1 production and the activation of AP-1 in vascular endothelial cells using heme oxygenase-1 (HO-1)-dependent mechanisms. 45,46 Apigenin is a flavone that is the aglycone of apiin isolated from parsley and celery.…”