Epigallocatechin-3-Gallate Regulates Anti-Inflammatory Action Through 67-kDa Laminin Receptor-Mediated Tollip Signaling Induction in Lipopolysaccharide-Stimulated Human Intestinal Epithelial Cells

Byun, Eui‐Baek; Kim, Woo Sik; Sung, Nak-Yun; Byun, Eui-Hong

doi:10.1159/000489447

Cited by 41 publications

(25 citation statements)

References 28 publications

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“…67LR is a membrane receptor of EGCG 22 and EGCG was reported to trigger cell death via 67LR in multiple types of tumour cells 23,36 . Previous studies showed that EGCG could activate the 67LR signalling pathway to inhibit inflammation in endothelial cells 27 , adipocytes 26 and intestinal epithelial cells 37 . EGCG remarkably decreased oxidative stress and inflammation levels through 67LR in an acute lung injury mouse model 24 , and EGCG inhibited H 2 O 2 -induced apoptosis via 67LR in mouse vascular smooth muscle cells, indicating that the antioxidant effect of EGCG is associated with 67LR 25 .…”

Section: Discussionmentioning

confidence: 99%

(−)-Epigallocatechin-3-gallate (EGCG) attenuates salt-induced hypertension and renal injury in Dahl salt-sensitive rats

Luo

Chen

et al. 2020

Sci Rep

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Epigallocatechin-3-gallate (EGCG), a main active catechin in green tea, was reported to attenuate renal injury and hypertension. However, its effects on salt-induced hypertension and renal injury remain unclear. In the present study, we explored its effects on hypertension and renal damage in Dahl rats with salt-sensitive hypertension. We found that EGCG could lower blood pressure after 6 weeks of oral administration, reduce 24 h urine protein levels and decrease creatinine clearance, and attenuate renal fibrosis, indicating that it could attenuate hypertension by protecting against renal damage. Furthermore, we studied the renal protective mechanisms of EGCG, revealing that it could lower malondialdehyde levels, reduce the numbers of infiltrated macrophages and T cells, and induce the apoptosis of NRK-49F cells. Considering that the 67 kD laminin receptor (67LR) binds to EGCG, its role in EGCG-induced fibroblast apoptosis was also investigated. The results showed that an anti-67LR antibody partially abrogated the apoptosis-inducing effects of EGCG on NRK-49F cells. In summary, EGCG may attenuate renal damage and salt-sensitive hypertension via exerting anti-oxidant, antiinflammatory, and apoptosis-inducing effects on fibroblasts; the last effect is partially mediated by 67LR, suggesting that EGCG represents a potential strategy for treating salt-sensitive hypertension.Salt-sensitive hypertension is characterized by a significant increase in blood pressure after high salt intake. Patients with this condition are at high risk for cardiovascular and renal morbidity and mortality. Renal injury plays a key role in the pathogenesis of salt sensitive hypertension 1 . Recently, the roles of oxidative stress and inflammatory cell infiltration in the kidney in the pathogenesis of salt-sensitive hypertension were verified 2,3 . Renal fibrosis is a common pathway for a variety of chronic kidney diseases to progress to end-stage renal failure 4 and excessive proliferation of renal interstitial fibroblasts participates in the development of renal fibrosis 5,6 .(−)-Epigallocatechin-3-gallate (EGCG) is the most active and abundant polyphenol in green tea, accounting for approximately 50% of green tea polyphenols 7 . Recently, EGCG has attracted interest due to its wide range of biological activities, such as its antioxidant, NO-scavenging, anti-inflammatory, anti-arthritic and apoptosis-inducing effects in multiple types of tumour cells 8,9 . EGCG benefits a broad spectrum of hypertension disorders, including renovascular hypertension 10 and spontaneous hypertension 11 . The renoprotective effect of EGCG has been reported in several renal disease models 12 , such as acute kidney injury 13 , cisplatin-induced nephrotoxicity 14 , obstructive nephropathy 15 , glomerulonephritis 16 , lupus nephritis 17 , diabetic nephropathy 18 , and high-fat diet-induced kidney injury 19 . However, the effects of EGCG on salt-induced hypertension and renal injury remain unclear.

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Section: Discussionmentioning

confidence: 99%

(−)-Epigallocatechin-3-gallate (EGCG) attenuates salt-induced hypertension and renal injury in Dahl salt-sensitive rats

Luo

Chen

et al. 2020

Sci Rep

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“…For instance, dietary naringenin induces T regulatory (Treg) cells binding to intestinal AhR [75]. Furthermore, EGCG is able to bind to the 67 kDa laminin receptor, the zeta-chain-associated 70kDa protein (ZAP-70), and the retinoic acid-inducible gene (RIG)-I, respectively [75][76][77]. Neutrophils, monocytes/macrophages, mast cells, and T cells express ZAP-70 [78,79].…”

Section: Receptors For Polyphenolsmentioning

confidence: 99%

Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

Magrone

Russo

et al. 2019

Antioxidants

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In this review, special emphasis will be placed on red grape polyphenols for their antioxidant and anti-inflammatory activities. Therefore, their capacity to inhibit major pathways responsible for activation of oxidative systems and expression and release of proinflammatory cytokines and chemokines will be discussed. Furthermore, regulation of immune cells by polyphenols will be illustrated with special reference to the activation of T regulatory cells which support a tolerogenic pathway at intestinal level. Additionally, the effects of red grape polyphenols will be analyzed in obesity, as a low-grade systemic inflammation. Also, possible modifications of inflammatory bowel disease biomarkers and clinical course have been studied upon polyphenol administration, either in animal models or in clinical trials. Moreover, the ability of polyphenols to cross the blood–brain barrier has been exploited to investigate their neuroprotective properties. In cancer, polyphenols seem to exert several beneficial effects, even if conflicting data are reported about their influence on T regulatory cells. Finally, the effects of polyphenols have been evaluated in experimental models of allergy and autoimmune diseases. Conclusively, red grape polyphenols are endowed with a great antioxidant and anti-inflammatory potential but some issues, such as polyphenol bioavailability, activity of metabolites, and interaction with microbiota, deserve deeper studies.

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“…Epigallocatechin-3-gallate found in green tea can regulates anti-inflammatory action through laminin receptor-mediated tollip signaling induction in LPS-stimulated human intestinal epithelial cells [157]. Epigallocatechin-3-gallate (25-50 µM) can also suppress nicotine-induced migration and invasion by blocking angiogenesis and EMT of non-small cell lung carcinoma (NSCLC) cells [158].…”

Section: Reversal Of Emt By Anti-inflammatory Natural Compoundsmentioning

confidence: 99%

Reversal of Epithelial–Mesenchymal Transition by Natural Anti-Inflammatory and Pro-Resolving Lipids

Lee

2019

Cancers

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Epithelial mesenchymal transition (EMT) is a key process in the progression of malignant cancer. Therefore, blocking the EMT can be a critical fast track for the development of anticancer drugs. In this paper, we update recent research output of EMT and we explore suppression of EMT by natural anti-inflammatory compounds and pro-resolving lipids.A typical signaling pathway of EMT is the transforming growth factor-β1 (TGF-β1) pathway. TGF-β1 induces EMT via SMAD-dependent or non-SMAD signaling pathway [7]. Growth factors including FGF, HGF, IGF1, EGF, and PDGF via receptor tyrosine kinase can induce EMT via signaling pathway of PI3K-AKT and ERK MAPK [8][9][10][11]. Wnt signaling, hedgehog signaling, Notch signaling, hypoxia, and inflammatory tumor microenvironment also involves in EMT [5]. Recently, it has been shown that hippo signaling is also involved in EMT [12]. YAP and TAZ can enhance EMT through upregulation of EMT transcription factors such as forkhead box C2 (FOXC2), snail family zinc finger 1/2 (SNAIL1, SLUG), twist-related protein 1 (TWIST1), and ZEB1 [12-15]. Transcription Factors Involved in EMTNovel players are newly recognized as regulatory transcription factor in the EMT. Brachyury, the T-box transcription factor, is a novel transcription factor implicated in the EMT of cancer cells [16]. Brachyury is known as the target gene of WNT, one of the m ajor signaling pathways of EMT [17]. Foxq1, one of forkhead transcription factor, has also regarded as a novel transcription factor mediating the EMT of gastric cancer [6,18]. Runt-related transcription factor 2 (Runx2) belongs to the runt-related transcription factor family [19]. Runx2 plays a key role in EMT of hepatocellular carcinoma (HCC) [20]. GATA transcription factors are also implicated in the EMT of cancer cells [21].A typical signaling pathway of EMT is the transforming growth factor-β1 (TGF-β1) pathway. TGF-β1 induces EMT via SMAD-dependent or non-SMAD signaling pathway [7]. Growth factors including FGF, HGF, IGF1, EGF, and PDGF via receptor tyrosine kinase can induce EMT via signaling pathway of PI3K-AKT and ERK MAPK [8][9][10][11]. Wnt signaling, hedgehog signaling, Notch signaling, hypoxia, and inflammatory tumor microenvironment also involves in EMT [5]. Recently, it has been shown that hippo signaling is also involved in EMT [12]. YAP and TAZ can enhance EMT through upregulation of EMT transcription factors such as forkhead box C2 (FOXC2), snail family zinc finger 1/2 (SNAIL1, SLUG), twist-related protein 1 (TWIST1), and ZEB1 [12-15]. Transcription Factors Involved in EMTNovel players are newly recognized as regulatory transcription factor in the EMT. Brachyury, the T-box transcription factor, is a novel transcription factor implicated in the EMT of cancer cells [16]. Brachyury is known as the target gene of WNT, one of the major signaling pathways of EMT [17]. Foxq1, one of forkhead transcription factor, has also regarded as a novel transcription factor mediating the EMT of gastric cancer [6,18]. Runt-related transcription factor 2 (Runx2...

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Epigallocatechin-3-Gallate Regulates Anti-Inflammatory Action Through 67-kDa Laminin Receptor-Mediated Tollip Signaling Induction in Lipopolysaccharide-Stimulated Human Intestinal Epithelial Cells

Cited by 41 publications

References 28 publications

(−)-Epigallocatechin-3-gallate (EGCG) attenuates salt-induced hypertension and renal injury in Dahl salt-sensitive rats

(−)-Epigallocatechin-3-gallate (EGCG) attenuates salt-induced hypertension and renal injury in Dahl salt-sensitive rats

Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

Reversal of Epithelial–Mesenchymal Transition by Natural Anti-Inflammatory and Pro-Resolving Lipids

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