Epigallocatechin‐3‐gallate inhibits the invasion of human oral cancer cells and decreases the productions of matrix metalloproteinases and urokinase‐plasminogen activator

Abstract

Taken together, these results suggest that EGCG could inhibit the invasion and migration of human oral cancer cells and that the effects may partially because of the decreased productions of MMP-2, MMP-9, and uPA.

Green tea (−)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met

“…We demonstrated that HGF-induced expression of MMP-2 and MMP-9 in KB cells was reduced by EGCG treatment in a dose-dependent manner. Similar results were found in that EGCG is a potent inhibitor of the expression and activity of MMP-2 and MMP-9 in HT1080 cells [19,38,39] and oral cancer cell line OEC-M1 cells [40]. Thus, inhibition of the migration or invasion mediated by MMP-2 or MMP-9 may be a key feature in the prevention of cancer invasion or metastasis.…”

“…Even in the presence of HGF, as the dose of EGCG increased, the number of TUNEL-positive cells increased dose-dependently. Interactions between cells with the extracellular matrix that promote adhesion and migration are believed to be essential for invasion, migration and metastasis of tumors [19]. MMP plays an important role in the processes of cancer invasion and metastasis.…”

“…The major activities of EGCG include inhibition of numerous signaling pathways and protein kinases (AP-1, p44/p42 MAPK, Erk1/2, EGF-R, PDGF-R, FGF-R), inhibition of cell proliferation, induction of apoptosis, modulation of cell cycle regulation, interference with receptor binding and suppression of invasiveness and angiogenesis [17,18]. Recently, some studies showed that EGCG could inhibit the invasion and migration of human oral cancer cells through multiple mechanisms, possibly by the decreased production of MMP (matrix metalloproteinase)-2, MMP-9 and uPA or the demethylation effect on MMP inhibitors, such as RECK [19,20]. Also, EGCG has been reported to inhibit ligandinduced c-Met phosphorylation and potentially block invasive cancer growth [4,21].…”

Telomerase activity in the occurrence and progression of oral squamous cell carcinoma

“…To date, many such molecules have been identified, including 3′-azido-3′-deoxythymidine (AZT), which inhibits human immunodeficiency virus (HIV)-1 by interfering with DNA synthesis (43), PIPER (N,N′-bis[2-(1-piperidino) ethyl]-3,4,9,10-perylenetetra-carboxylic diimide), which binds to G-quadruplexes (44), and epigallocatechin-3-gallate (EGCG), which is derived from polyphenols in green tea (45). Of these, EGCG has been reported to inhibit the invasion and proliferation of oral cancer cells (46,47). In addition, there is growing support for the use of NaB as an anti-cancer agent.…”

Bak is a key molecule in apoptosis induced by methanol extracts of Codonopsis lanceolata and Tricholoma matsutake in HSC-2 human oral cancer cells

“…Numerous studies have demonstrated that natural products play critical roles against oral cancer. For example, green tea extracts (GTEs) reduce the incidence of oral cancer development by inhibiting invasion and metastasis (19,20). Withania somnifera extract also reduces the overall occurrence of oral tumors by increasing antioxidant activity (21,22).…”