Epigallocatechin‐3‐gallate inhibits proliferation and induces apoptosis in odontogenic keratocyst keratinocytes

Abstract

Objective The aim of this study was to investigate the effects of epigallocatechin‐3‐gallate on the proliferation and apoptosis of odontogenic keratocyst (OKC) keratinocytes in vitro. Materials and methods Keratinocytes isolated from the epithelial lining of the OKC were cultured in keratinocyte serum‐free medium and identified by CK10, CK14, pan‐cytokeratin and vimentin immunofluorescence staining. The cells were exposed to EGCG at different concentrations, and proliferation inhibition was measured by cell counting kit 8 assay. Cell cycle and apoptosis were assessed by flow cytometry, and expression of the WNT signalling pathway‐related proteins FZD3 and JNK3 was detected by quantitative real‐time PCR and Western blotting. Human oral keratinocytes (HOKs) were used as the control. Results The OKC keratinocytes were successfully cultured. The primary cells were tile‐like and expressed the epithelial biomarkers CK10, CK14 and pan‐cytokeratin. Epigallocatechin‐3‐gallate inhibited cell proliferation in a dose‐ and time‐dependent manner, arrested cell cycle in the G1 phase and induced apoptosis of OKC keratinocytes. FZD3 and JNK3 were overexpressed in OKC keratinocytes compared with HOKs and were downregulated by epigallocatechin‐3‐gallate treatment. Conclusion Epigallocatechin‐3‐gallate inhibited proliferation and induced apoptosis in OKC keratinocytes, possibly by suppressing the WNT/JNK signalling pathway. It may thus be potentially used for OKC treatment.

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“…Catechin is a substance abundant in green tea and many studies approved its anticancer effects (Buttet al, 2015;Ohishi et al, 2016). These effects can be due to its role in apoptotic and signaling pathways (Kumar et al, 2019;Yang et al, 2019). The cytotoxic and apoptotic effects of S. rhombifolia extract may be attributed to the presence of epicatechin, why so it has also been present in S. rhombifolia to a great extent than other compounds and its apoptotic effects have been confirmed in other studies.…”

Epigallocatechin gallate (EGCG) alleviates the inflammatory response and recovers oral microbiota in acetic acid-induced oral inflammation mice

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The therapeutic effects and antioxidant properties of epigallocatechin-3 gallate: A new review

“…Cerezo-Guisado et al (2015) found that EGCG induces cell death in colon cancer cell line (HT-29 cells) and it has cytotoxic effects and has a cancer preventive role. Yang et al (2019) stated that EGCG induces apoptosis by inhibiting proliferation in odontogenic keratocyst keratinocytes by suppressing the WNT/JNK signaling pathway. Jankun et al (2014) stated that EGCG had better efficacy to prevent tumor cell growth than mitomycin C (MMC) when they compared EGCG and MMC in rats with superficial bladder cancer.…”