(−)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice

Sae-tan, Sudathip; Grove, Kimberly A.; Kennett, Mary J.; Lambert, Joshua D.

doi:10.1039/c0fo00155d

Cited by 95 publications

(77 citation statements)

References 25 publications

(50 reference statements)

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“…This finding is in agreement with reported data about high doses of pure green tea catechins: EGCG, EGC and epicatechine that effectively reduced adipose tissue mass and ameliorated plasma lipid profiles in high-fat diet induced obese mice [14,15]. They are also consistent with those indicating that a solution containing variable doses of EGCG or EGC or both was able to reduce body weight gain, fasting blood glucose, plasma insulin, insulin resistance and some markers of obesity-related fatty liver disease [16]. Therefore, our findings confirm that 15-min GTD has equivalent effects than those reported with purified catechins used in different experimental conditions.…”

Section: Discussionsupporting

confidence: 82%

Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats

Abid¹

2015

IJNFS

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Background: In previous studies, we reported that green tea decoction (GTD) cooked in boiling water for 15-min (15-min GTD) reduced body weight gain (BWG), glucose and lipid absorption and improved total antioxidant status (TAS) of rats fed normal or high-fat diet (HFD). However, the long-term effect of a mixture: 15-min GTD + lemon juice (LJ) on BWG, liver steatosis (LS), TAS, blood glucose and lipids is still unknown. Purpose The aim of the present study is to examine the long-term effect of 15-min GTD + LJ mixture on above parameters in rats fed HFD induced-obesity and LS. Methods Twentyeight male rats were assigned to four groups with comparable body weights. During 9-weeks treatment, rats were given adlibitum a basic diet (BD) or HFD to promote obesity and LS. The group 1 received the BD (BD group), the group 2 received HFD (HFD group), the group 3 received HFD + 15-min GTD (GTD group) and the group 4 received HFD + a mixture: 15-min GTD and LJ (GTDLJ group). Results The rats fed HFD had the highest BWG associated with an abundant LS spread than rats consumed BD. However, 15-min GTD significantly reduced the BWG and LS spread (p<0.001). GTD group showed also decreased plasma glucose, triglycerides and increased both TAS and GPX activities (p<0.05 and p<0.01, respectively). Surprisingly, an abundant reappearance of several LS spread and a restore of BWG were observed in GTDLJ group compared to GTD group. The GTDLJ mixture also reversed the GTD effect on plasma glucose, TAS, GPX and blood iron. Conclusion These results show that 15-min GTD significantly reduces BWG, HFD induced-LS, plasma glucose, triglycerides, blood iron and improves TAS and GPX. However, the GTDLJ mixture partially or totally counteracts the effect of 15-min GTD on BWG, LS, plasma glucose, blood iron, TAS and GPX.

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Section: Discussionsupporting

confidence: 82%

Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats

Abid¹

2015

IJNFS

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“…This suggests that EGCG is an effective treatment for NAFLD mice. Recently, several studies confirmed that the beneficial effects of EGCG on NAFLD may be attributed to its induction of hepatic autophagy and the attenuation of inflammation and oxidative stress [23][24][25] . Insulin resistance is closely associated with NAFLD and is a key factor in the pathogenesis and potential evolution of NAFLD [1,2] .…”

Section: Discussionmentioning

confidence: 99%

Green tea polyphenol epigallocatechin-3-gallate ameliorates insulin resistance in non-alcoholic fatty liver disease mice

et al. 2015

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Aim: Epigallocatechin-3-gallate (EGCG) is a major polyphenol in green tea. In this study, we investigated the effects of EGCG on insulin resistance and insulin clearance in non-alcoholic fatty liver disease (NAFLD) mice. Methods: Mice were fed on a high-fat diet for 24 weeks. During the last 4 weeks, the mice were injected with EGCG (10, 20 and 40 mg·kg -1 ·d -1 , ip). Glucose tolerance, insulin tolerance and insulin clearance were assessed. After the mice were euthanized, blood samples and tissue specimens were collected. Glucose-stimulated insulin secretion was examined in isolated pancreatic islets. The progression of NAFLD was evaluated histologically and by measuring lipid contents. Insulin-degrading enzyme (IDE) protein expression and enzyme activity were detected using Western blot and immunocapture activity assays, respectively. Results: The high-fat diet significantly increased the body weight and induced grade 2 or 3 liver fatty degeneration (steatosis, lobular inflammation and ballooning) accompanied by severe hyperlipidemia, hyperglycemia, hyperinsulinemia and insulin resistance in the model mice. Administration of EGCG dose-dependently ameliorated the hepatic morphology and function, reduced the body weight, and alleviated hyperlipidemia, hyperglycemia, hyperinsulinemia and insulin resistance in NAFLD mice. Furthermore, EGCG dosedependently enhanced insulin clearance and upregulated IDE protein expression and enzyme activity in the liver of NAFLD mice. Conclusion: EGCG dose-dependently improves insulin resistance in NAFLD mice not only by reducing body weight but also through enhancing the insulin clearance by hepatic IDE. The results suggest that IDE be a potential drug target for the treatment of NAFLD.

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“…Previous reports have indicated that dietary GTE and FO can reduce body weight by increasing fat oxidation (Rains et al, 2011;Martínez-Victoria and Yago, 2012;Buckley and Howe, 2010;Sae-Tan et al, 2011). Previously, we showed that FO intake also reduced Hg accumulation in muscle and led to loss of body weight in MeHg-exposed mice (Shirai et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Effects of Green Tea Extracts and Fish Oil on Mercury Accumulation and Antioxidant Defenses in Methylmercury-exposed Adult Mice

Shirai

Yamashita

2013

FSTR

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Male mice (4 months old) were fed green tea extract-(GTE; 0.25% w/w) and/or fish oil-(FO; 5% w/w) containing diets with 8 ppm methylmercury (MeHg) chloride for 4 months to investigate the effects of simultaneous intakes on brain functions, antioxidant defenses against MeHg, and MeHg accumulation in tissues. In mouse maze tests, intake of GTE or FO significantly improved the learning ability of MeHg-exposed mice. GTE and FO also significantly decreased liver and brain catalase activities in MeHg-exposed mice. Total Hg concentrations in muscle were significantly lowered by dietary GTE and FO in the MeHgexposed group, though no remarkable differences were observed in the brain. These data indicate that simultaneous intake of GTE and FO effectively prevents MeHg-mediated oxidative stress and reduces the effects of Hg exposure with fish consumption.Keywords: fish oil, green tea, methylmercury, ascorbic acid, thiobarbituric acid reactive substances *To whom correspondence should be addressed. E-mail: nshinya@affrc.go.jp IntroductionFish contains several nutrients that are beneficial to human health. However, fish has been known to contain hazardous components such as methylmercury (MeHg), which has been linked to decreased neuropsychological function and increased risk of cardiovascular diseases (Choi et al., 2009;Virtanen et al., 2007). In contrast, n-3 polyunsaturated fatty acids (PUFA) contained in fish improve brain function (Guesnet et al., 2011;Mourek and Mourek, 2011) and reduce the risk of cardiovascular diseases (Cottin et al., 2011). Some reports indicate that dietary fat can influence oxidative DNA damage, nephrotoxicity, and steroidogenic enzyme activities after MeHg exposure (Grotto et al., 2011;Jin et al., 2008Jin et al., , 2009McVey et al., 2008). However, the human diet varies widely, and some reports have shown that foodstuffs other than seafood may also prevent MeHg toxicity and accumulation (Abdalla et al., 2010;Farina et al., 2005;Lee et al., 1999;Rowland et al., 1986;Sumathi et al., 2012). Therefore, daily fish consumption may alleviate relatively few health risks.In general, the Japanese drink a few cups of green tea every day, and often accompany sushi meals with green tea. Therefore, a close relationship exists between green tea and fish oil in the Japanese diet. Canuel et al. (2006) concluded that tea might accelerate the enterohepatic MeHg cycle and contribute to temporary MeHg bioamplification in the bloodstream. Green tea contains large quantities of polyphenols that have antioxidant effects and accelerate cadmium (Cd) excretion by forming chelates (Abib et al., 2011;Yu et al., 2007;Wang et al., 2012). Some studies indicate that green tea consumption reduces the risk of coronary heart disease (Bøhn et al., 2012;Chacko et al., 2010) and prevents agerelated neurodegeneration (Andrade and Assunção, 2012). Thus, these green tea polyphenol properties may be effective against MeHg toxicity.The aim of this study was to determine the interactive influence of fish oil and green tea on the effects o...

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(−)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice

Cited by 95 publications

References 25 publications

Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats

Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats

Green tea polyphenol epigallocatechin-3-gallate ameliorates insulin resistance in non-alcoholic fatty liver disease mice

Simultaneous Effects of Green Tea Extracts and Fish Oil on Mercury Accumulation and Antioxidant Defenses in Methylmercury-exposed Adult Mice

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