Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression

Abstract

Epigallocatechin-3-gallate (EGCG) is a kind of polyphenol compound, called catechin, and is extracted from green tea. EGCG has a wide range of biological activities. The present study aimed to evaluate the effect of EGCG on neointimal hyperplasia in a rat model of carotid artery balloon injury and to explore the molecular mechanisms involved. Various experiments were performed to assess the effects of EGCG on thickening of neointima, expression levels of high mobility group box 1 protein (HMGB1) and receptor of advanced glycation end products (RAGE), the inflammatory response, oxidative stress and activation of nuclear factor (NF)-κB. Results demonstrated that EGCG decreased the intimal area and the ratio of intimal area/medial area compared with the balloon injury group. The expression levels of HMGB1 and RAGE induced by balloon injury were markedly inhibited by EGCG treatment. Furthermore, the inflammatory response and oxidative stress damage, which have close correlations with HMGB1, were restrained by EGCG. Finally, EGCG treatment markedly inhibited NF-κB activation. The present data provided evidence that EGCG attenuates neointimal hyperplasia in a model of carotid artery balloon injury, which indicated that EGCG may serve as a potential drug for restenosis in clinics.

Cytokine‐induced apoptosis inhibitor 1 (CIAPIN1) accelerates vascular remodelling via p53 and JAK2‐STAT3 regulation in vascular smooth muscle cells

“…In addition, cytoplasmic CIAPIN1 is actively translocated into the nucleus by reactive oxygen species (ROS)-inducing 6-hydroxydopamine in dopaminergic neuronal cells (MN9D) and rat brain (Park et al, 2011). Since vascular injury conditions such as balloon injury and high level of PDGF-BB increased ROS level (Dong et al, 2020;B. Yang et al, 2017), we investigated localisation of CIAPIN1 in vascular injury condition.…”

Epigallocatechin Gallate (EGCG) Improves Anti-Angiogenic State, Cell Viability, and Hypoxia-Induced Endothelial Dysfunction by Downregulating High Mobility Group Box 1 (HMGB1) in Preeclampsia

“…EGCG is the most abundant catechin in green tea and has a variety of biological activities [ 12 ]. A study suggested that EGCG can improve the treatment efficacy of oral nifedipine against severe PE [ 13 ], and EGCG treatment can markedly inhibit the expression of HMGB1 induced by balloon injury [ 16 ]. EGCG can alleviate lung injury by inhibiting the expression of HMGB1 and RAGE [ 17 ].…”

“…EGCG can enhance the endothelial nitric oxide synthase (eNOS) activation and nitric oxide (NO) formation in HUVECs, indicating that EGCG may help improve endothelial functions [ 15 ]. Several studies showed that EGCG can inhibit the expression of HMGB1 [ 16 , 17 ].…”

Potential protective mechanisms of green tea polyphenol EGCG against COVID-19

“…downregulates pancreatic RAGE in diabetic mice ( Feng, Hou, Zhu, Zhu, & Jiang, 2019 ); 3) EGCG (2 mg/kg and 4 mg/kg, i.p.) improves restenosis in a rat model of carotid artery balloon injury via inhibiting HMGB1/RAGE and NF-κB activation ( Yang et al, 2017 ); 4) EGCG (75 mg/kg, i.p. 3 times per week) alleviates hyperglycemia, downregulates RAGE expression and reduces advanced glycation end products in mice with high fat diet-induced obesity ( Sampath, Rashid, Sang, & Ahmedna, 2017 ); 5) in patients with type II diabetes, EGCG at doses from 300 to 900 mg/day dose-dependently increases plasma levels of soluble RAGE, a RAGE variant acting as a ligand decoy that competes with RAGE ( Huang et al, 2013 ).…”