Epigallocatechin-3-gallate attenuates head and neck cancer stem cell traits through suppression of Notch pathway

Lee, Sang Hyuk; Nam, Hyo Jung; Kang, Hyun Jung; Kwon, Hye Won; Lim, Young Chang

doi:10.1016/j.ejca.2013.06.025

Cited by 97 publications

(75 citation statements)

References 32 publications

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“…It's well known, analysis the capacity to form multi-cellular spheroids is a commonly used technique to evaluate the self-renewal potential of CSCs in many solid tumors [12]. In our present study, we found that the ability to form spheroids in sphere culture was markedly restricted after restoration of miR-200a expression.…”

Section: Discussionsupporting

confidence: 60%

Upregulation of microRNA-200a associates with tumor proliferation, CSCs phenotype and chemosensitivity in ovarian cancer

Liu¹,

Zhong²,

Zeng³

et al. 2015

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Ovarian cancer is a lethal gynecologic malignancy and always has a poor prognosis. Despite new treatments modalities, the long term outcomes had not been significantly improved in the past 30 years. Although has been reported to be a prognostic marker in ovarian cancer, it's exact role in ovarian cancer remain unclear. In this study, we inserted the response element of miR-200a in ovarian cancer cell line via lentivirus-mediated transgene in vitro, and qRT-PCR (real time quantitative reverse transcription PCR) assay confirmed that miR-200a was up regulated compared with control. Then colony-formation assay, cell cycle analysis, CCK8 assays in vitro and xenograft experiments in vivo were performed and verified that miR-200a promoted proliferation, while blocked the formation of tumor spheroids and reduced the ratio of SP (side population) cells in ovarian cancer. Finally, we validated that miR-200a significantly enhanced the chemosensitivity of paclitaxel but not cisplatin in both adherent culture and sphere culture. Taken together, we demonstrated that upregulation miR-200a promoted proliferation and inhibited cancer stem cells (CSCs) phenotype in OVCAR-3 ovarian cancer cell line, combined with cell cycle-targeting drug paclitaxel could effectively eliminate the "side effects" of proliferation, and showed evidences that this strategy may be promising for ovarian cancer treatment. Key words: microRNA, ovarian cancer, proliferation, cancer stem cells, chemosensitivityOvarian cancer is still the most lethal gynaecologic malignancy according to 2013 statistics [1]. Although the current standard treatment, systemic by administration of platinum and paclitaxel after debulking surgery was efficient in almost 70% of ovarian cancer cases, most of these patients ultimately develop chemoresistance and recurrence [2].Recently, accumulated evidence showed that the miR-200a expression level was closely associated with progression free survival (PFS), overall survival (OS) and chemo-sensitivity in ovarian cancer, thus miR-200a was suitable as a prognostic marker for ovarian cancer outcome [3,4]. Previously, we demonstrated that the profile of miRNAs was different between CD133+ and CD133 -ovarian cancer cells, miR-200a was down-regulated in CD133 + ovarian cancer stem cells (CSCs) [5], and upregulation miR-200a could reduce ZEB2-mediated migration and invasion of CD133 + ovarian CSCs [6]. However, miR-200a regulation of proliferation, CSCs phenotype and chemosensitivity in ovarian cancer remain unclear. Therefore, an in-depth understanding of the roles and mechanisms of miR-200a in ovarian cancer is needed.Herein, we first established that miR-200a was stably overexpressed in OVCAR-3 ovarian cancer cell line and then validated that over-expression of miR-200a increased tumor proliferation and tumor volume. Additionally, we found that the over-expression of miR-200a weakened the CSCs phenotype by blocking the formation of tumor spheroids and reducing the ratio of side population (SP) cells. Importantly, we demonstrated miR-200...

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Section: Discussionsupporting

confidence: 60%

Upregulation of microRNA-200a associates with tumor proliferation, CSCs phenotype and chemosensitivity in ovarian cancer

Liu¹,

Zhong²,

Zeng³

et al. 2015

neo

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“…SOX2 expression has been demonstrated to improve the ability of invasion and migration of tumor cells in tongue squamous cell carcinoma (TSCC) (77). Other properties of cancer cells in OSCC or HNSCC that involve the SOX2 protein include apoptosis (74,78), chemoresistance (79), metastasis and tumorigenesis (10,79).…”

Section: Sox2 In Osccmentioning

confidence: 99%

Expression of SOX2 in oral squamous cell carcinoma and the association with lymph node metastasis

Ren

Zhang

2016

Oncology Letters

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Abstract. Oral squamous cell carcinomas (OSCCs) are a growing problem in the world. The various existing treatments have not markedly improved the survival rate of patients with OSCC during the past three decades. Novel treatment strategies are required. Sex determining region Y-box 2 (SOX2) is a transcription factor that is involved in the maintenance of embryonic stem cell pluripotency and in multiple developmental processes. SOX2 expression was indicated to act as a prognostic factor in various types of tumors, including breast, colorectal, gastric and lung cancer and glioblastoma, and as a link between malignancy and stemness. Cancer stem cells (CSCs) may be responsible for the genesis, growth and metastatic spread of tumors. The poor survival outcomes for OSCC patients may be attributable to a poor selection of target cells for treatment, as current oral cancer therapies are generally aimed at the global mass of tumor. Therefore, the consideration that novel approaches to oral cancer may be targeted using SOX2 and CSCs appears reasonable. In order to better understand the oncogenic roles and the corresponding signal transduction pathways of the SOX2 protein, the present study emphasizes the role of SOX2 in OSCC, including the proteins associated with OSCC, and reviews the literature regarding the role of SOX2 in lymph node metastasis. The aim of the present study is to provide a reference for future studies that engage in research on the aforementioned subject.

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“…It has been also shown that synergistic treatment of EGCG with quercetin led to a decrease of CD44+/ CD133 + prostate cells through inhibition of Vimentin, Slug, Snail, and nuclear b-catenin expression and LEF-1/TCF responsive reporter activity, affecting the CSC migration and invasion (Tang et al, 2010), and in combination with curcumin, it led to a reduction of sphere formation from breast tumor cells by inhibition of STAT3 phosphorylation and retention of STAT3-NFkB interaction (Chung & Vadgama, 2015). Moreover, in head and neck squamous cell carcinoma (HNSCC), EGCG treatment has been reported to downregulate the expression of stem cell markers, such as Oct4, Sox2, Nanog, and CD44 and to suppress HNSCC cells sphere forming capacity, augmenting their cisplatin (CP)-mediated chemosensitivity by inhibiting ABCC2 and ABCG2 transporter genes (Lee et al, 2013b). In the colorectal tumor model, EGCG induced sensitization of the 5-fluorouracil (5FU)-resistant colorectal cancer cells and spheroid-derived colorectal CSCs to 5FU treatment that was associated with suppression of Notch1, Bmi1, Suz12, and Ezh2, upregulation of self-renewal suppressive-miRNAs, miR-34a, miR-145, and miR-200c and enhancement of apoptosis and cell-cycle arrest (Toden et al, 2016).…”

Section: Anticancer Activity Of Polyphenol: Modulation Of Cell Redox mentioning

confidence: 99%

Polyphenols delivery by polymeric materials: challenges in cancer treatment

et al. 2017

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Nanotechnology can offer different solutions for enhancing the therapeutic efficiency of polyphenols, a class of natural products widely explored for a potential applicability for the treatment of different diseases including cancer. While possessing interesting anticancer properties, polyphenols suffer from low stability and unfavorable pharmacokinetics, and thus suitable carriers are required when planning a therapeutic protocol. In the present review, an overview of the different strategies based on polymeric materials is presented, with the aim to highlight the strengths and the weaknesses of each approach and offer a platform of ideas for researchers working in the field.

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Epigallocatechin-3-gallate attenuates head and neck cancer stem cell traits through suppression of Notch pathway

Cited by 97 publications

References 32 publications

Upregulation of microRNA-200a associates with tumor proliferation, CSCs phenotype and chemosensitivity in ovarian cancer

Upregulation of microRNA-200a associates with tumor proliferation, CSCs phenotype and chemosensitivity in ovarian cancer

Expression of SOX2 in oral squamous cell carcinoma and the association with lymph node metastasis

Polyphenols delivery by polymeric materials: challenges in cancer treatment

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