Epididymal protease inhibitor (EPPIN) is a protein hub for seminal vesicle-secreted protein SVS2 binding in mouse spermatozoa

Mariani, Noemia Aparecida Partelli; Camara, Aline C.; Silva, Alan Andrew S.; Raimundo, Tamiris R F; Andrade, Juliana J.; Andrade, Alexandre Carvalho de; Rossini, Bruno César; Marino, Celso Luís; Kushima, Hélio; Santos, Lucilene Delazari dos; Silva, Erick J. R.

doi:10.1016/j.mce.2020.110754

Cited by 11 publications

(2 citation statements)

References 35 publications

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“…SVS3 showed affinity for SVS2 and facilitated the effects of SVS2 on sperm capacitation [ 53 ]. Epididymal protease inhibitor (EPPIN) directly binds SVS2, while indirectly interacting with SVS3A, SVS5, and SVS6 via SVS2 in mice [ 54 ]. As the most biochemically complex ion channel known to date, the CatSper channel complex consist of seven protein components (CatSper1-4, β, γ, and δ) derived from mouse testis [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

A New Gene SCY3 Homologous to Scygonadin Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of Scylla paramamosain

Chen

et al. 2023

IJMS

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In the study, a new gene homologous to the known antimicrobial peptide Scygonadin was identified in mud crab Scylla paramamosain and named SCY3. The full-length sequences of cDNA and genomic DNA were determined. Similar to Scygonadin, SCY3 was dominantly expressed in the ejaculatory ducts of male crab and the spermatheca of post-mating females at mating. The mRNA expression was significantly up-regulated after stimulation by Vibrio alginolyticus, but not by Staphylococcus aureus. The recombinant protein rSCY3 had a killing effect on Micrococcus luteus and could improve the survival rate of mud crabs infected with V. alginolyticus. Further analysis showed that rSCY3 interacted with rSCY1 or rSCY2 using Surface Plasmon Resonance (SPR, a technology for detecting interactions between biomolecules using biosensor chips) and Mammalian Two-Hybrid (M2H, a way of detecting interactions between proteins in vivo). Moreover, the rSCY3 could significantly improve the sperm acrosome reaction (AR) of S. paramamosain and the results demonstrated that the binding of rSCY3, rSCY4, and rSCY5 to progesterone was a potential factor affecting the sperm AR by SCYs on. This study lays the foundation for further investigation on the molecular mechanism of SCYs involved in both immunity and physiological effects of S. paramamosain.

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Section: Discussionmentioning

confidence: 99%

A New Gene SCY3 Homologous to Scygonadin Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of Scylla paramamosain

Chen

et al. 2023

IJMS

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“…Recent research has suggested that EPPIN inhibits sperm capacitation and sperm motility by binding clusterin (CLU) and lactotransferrin (LTF) to form a shell protein on the surface of sperm. The expression of EPPIN is negatively correlated with sperm concentration and sperm motility (Mariani et al., 2020). The 34‐kDa human epididymal sperm protein, P34H, mostly binds to the spermatoacrosome site and is involved in mediating interactions between sperm and the zona pellucida.…”

Section: Introductionmentioning

confidence: 99%

Study of microRNA Expression Profile in Different Regions of Ram Epididymis

Wang

Zhai

et al. 2021

Reprod Domestic Animals

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The regional expression of epididymal genes provides a guarantee for sperm maturation. As a class of endogenous non‐coding small RNAs, microRNAs (miRNAs) play an important role in spermatogenesis, maturation and fertilization. Currently, the regulatory role of miRNA in the epididymis is poorly understood. Here, transcriptome sequencing was used to analyse miRNA expression profiles in three regions of the epididymis of rams, including caput, corpus and cauda. The results showed that there were 13 known miRNAs between the caput and corpus controls, 29 between the caput and cauda and 22 differences between the corpus and cauda. Based on the analysis of miRNA target genes by GO and KEGG, a negative regulation network of miRNA–mRNA was constructed in which let‐7, miR‐541‐5p, miR‐133b and miR‐150 may play an important regulatory role in the maturation regulation of ram epididymal sperm. This research provides a reference for studying the regulation mechanism of sperm maturation in male epididymis and improving semen quality and male reproductive performance.

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Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta 1

Munipalli

Yenugu

2023

Reprod. Sci.

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Epididymal protease inhibitor (EPPIN) is a protein hub for seminal vesicle-secreted protein SVS2 binding in mouse spermatozoa

Cited by 11 publications

References 35 publications

A New Gene SCY3 Homologous to Scygonadin Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of Scylla paramamosain

A New Gene SCY3 Homologous to Scygonadin Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of Scylla paramamosain

Study of microRNA Expression Profile in Different Regions of Ram Epididymis

Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta 1

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