Epididymal Based Changes in Spermatozoa of Dromedary Camels

Rashad, Dina E.M.; Kandiel, Mohamed M.M.; El-Khawagah, Ahmed R.M.; Mahmoud, Karima Gh. M.; Ahmed, Yousef F.; El-Roos, Mahmoud E.A. Abou; Sosa, Gamal A.

doi:10.5958/2277-8934.2022.00033.9

Cited by 1 publication

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current results revealed that spermatozoa motility, count, viability and normal morphology were elevated in the high‐fertile group compared to the lower one. The cauda epididymal sperm cells motility, count, viability and normal morphology in our study nearly matches the records of Zeitoun and Mansour (2023) for fertile and infertile ejaculated camel semen ensuring the suitability of cauda semen for assisted reproductive techniques in camel (Rashad et al., 2022). Furthermore, the cauda epididymal spermatozoa features (count, motility and viability) nearly similar to Ali et al.…”

Section: Discussionsupporting

confidence: 88%

“…*, ** and *** indicated significant differences at p < .05, p < .01 and .001, respectively. epididymal sperm cells motility, count, viability and normal morphology in our study nearly matches the records ofZeitoun and Mansour (2023) for fertile and infertile ejaculated camel semen ensuring the suitability of cauda semen for assisted reproductive techniques in camel(Rashad et al, 2022). Furthermore, the cauda epididymal spermatozoa features (count, motility and viability)F I G U R E 2The expression profile of the candidate genes in camel bull epididymis (phospholipase A2 group IVD (PLA2G4D), lipocalin 15 (LCN15), clusterin associated protein 1 (CLUAP1), secreted phosphoprotein 1 (SPP1), aquaporin 12B (AQP12B), defensin beta 110 (DEFB110) and oestrogen receptor 1 (ER1).…”

supporting

confidence: 83%

“…After slaughtering, samples were transported in chilled physiological saline (0.9%) to the laboratory, where each epididymis was separated from the testicles, washed with physiological saline and ethyl alcohol 70%. The three epididymal segments (caput, corpus and cauda) were ascertained depending on their anatomical features (Rashad et al., 2022). Based on the epididymal spermatozoa characteristics (motility proportion, count, viability and normal morphology), samples were categorized into high‐ and low‐fertile groups (Rashad et al., 2024; Waheed et al., 2014).…”

Section: Methodsmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

See 3 more Smart Citations

Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

Rashad,

Ibrahim,

El‐Sokary

et al. 2024

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes (PLA2G4D, LCN15, CLUAP1, SPP1, AQP12B, DEFB110 and ESR1) relevant to spermatozoa features between the different epididymal segments in the mature dromedary camels (n = 30). Scrotal contents were collected post‐slaughtering, during the breeding season and the epididymis was separated from the testicles and divided into three segments (caput, corpus and cauda) based on its morphology and anatomical characteristics. Epididymal spermatozoa were harvested from each epididymal portion and evaluated for motility, count, viability and morphology. Samples were grouped depending on their epididymal sperm cells features into high‐fertile (n = 15) and low‐fertile (n = 15) groups. The gene expression of the candidate genes was defined in the isolated RNA from each epididymal portion tissue. The segmental sperm motion and count were significantly (p < .05 and p < .01) higher in the three epididymal parts of high‐fertile camels than the lower ones. There were some candidate genes markedly up‐regulated in its expression in epididymal head of high‐fertile camels (PLA2G4D and LCN15) and low fertile (CLUAP1), while others in the body region of the high‐fertile group (SPP1, AQP12B and DEFB110). Nevertheless, ER1 did not differ in the expression among the epididymal segments. In conclusion, the variant expression patterns of these epididymal genes in relation to the regional spermatozoa features might suggest important roles of these genes in sperm maturation process in the epididymis and focusing more interest on their potential utility as markers for male camel fertility prediction.

show abstract

Section: Discussionsupporting

confidence: 88%

supporting

confidence: 83%