Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling

Yang, Wen-Chieh; Chueh, Yi-Ru; Cheng, Ying-Ju; Siegenthaler, Dominique; Pielage, Jan; Chien, Ching‐Te

doi:10.1016/j.cub.2019.03.050

Cited by 14 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We further assessed the ultrastructural organization of microtubules by transmission electron microscopy (TEM). We recognized dendrites that locate between epidermal cells and the extracellular matrix in the dorsal field (Yang et al, 2019). In longitudinal sections, wild-type microtubules are aligned and cross-connected (n = 38) ( Figure 5E).…”

Section: Cct Subunits Localize To Dendrites and Regulate Dendritic MImentioning

confidence: 98%

“…Primary antibodies used in immunostaining included rat anti-CCT1 (1:50, Abcam), mouse anti-Futsch (1:200, Developmental Studies Hybridoma Bank), rabbit anti-GFP-Alexa488 (1:500, Life Technologies), and rabbit anti-HA (1:400; Cell Signaling Technology). Goat anti-rat Cy3 and anti-mouse Cy3 secondary antibodies were obtained from Jackson ImmunoResearch Laboratories Inc. Wandering larvae were dissected and prepared for TEM experiments as described previously (Yang et al, 2019).…”

Section: Immunohistochemistry and Temmentioning

confidence: 99%

See 1 more Smart Citation

An Efficient Screen for Cell-Intrinsic Factors Identifies the Chaperonin CCT and Multiple Conserved Mechanisms as Mediating Dendrite Morphogenesis

Wang

Ding

Cheng

et al. 2020

Front. Cell. Neurosci.

Self Cite

View full text Add to dashboard Cite

Dendritic morphology is inextricably linked to neuronal function. Systematic large-scale screens combined with genetic mapping have uncovered several mechanisms underlying dendrite morphogenesis. However, a comprehensive overview of participating molecular mechanisms is still lacking. Here, we conducted an efficient clonal screen using a collection of mapped P-element insertions that were previously shown to cause lethality and eye defects in Drosophila melanogaster. Of 280 mutants, 52 exhibited dendritic defects. Further database analyses, complementation tests, and RNA interference validations verified 40 P-element insertion genes as being responsible for the dendritic defects. Twenty-eight mutants presented severe arbor reduction, and the remainder displayed other abnormalities. The intrinsic regulators encoded by the identified genes participate in multiple conserved mechanisms and pathways, including the protein folding machinery and the chaperonin-containing TCP-1 (CCT) complex that facilitates tubulin folding. Mutant neurons in which expression of CCT4 or CCT5 was depleted exhibited severely retarded dendrite growth. We show that CCT localizes in dendrites and is required for dendritic microtubule organization and tubulin stability, suggesting that CCT-mediated tubulin folding occurs locally within dendrites. Our study also reveals novel mechanisms underlying dendrite morphogenesis. For example, we show that Drosophila Nogo signaling is required for dendrite development and that Mummy and Wech also regulate dendrite morphogenesis, potentially via Dppand integrin-independent pathways. Our methodology represents an efficient strategy for identifying intrinsic dendrite regulators, and provides insights into the plethora of molecular mechanisms underlying dendrite morphogenesis.

show abstract

Section: Cct Subunits Localize To Dendrites and Regulate Dendritic MImentioning

confidence: 98%

Section: Immunohistochemistry and Temmentioning

confidence: 99%

An Efficient Screen for Cell-Intrinsic Factors Identifies the Chaperonin CCT and Multiple Conserved Mechanisms as Mediating Dendrite Morphogenesis

Wang

Ding

Cheng

et al. 2020

Front. Cell. Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…L1CAM/Neuroglian (Nrg) likewise regulates dendrite positioning of Drosophila SSN dendrites, albeit by a slightly different mechanism. In flies, epidermal cells and SSNs express Nrg isoforms which differ in intracellular but not extracellular domains and are therefore capable of interacting (Yamamoto et al, 2006;Yang et al, 2019). A series of genetic manipulations of neuronal (Nrg180) or epidermal (Nrg167) isoforms demonstrated that dendrite spacing in the epidermis critically depends on the balance of Nrg dendrite-dendrite and dendrite-epidermis interactions (Yang et al, 2019).…”

Section: Direct Adhesive Interactions That Position Somatosensory Neuritesmentioning

confidence: 99%

“…Finally, junctional proteins are recruited to sheaths, where they may seal sheaths and limit sheath permeability (Kim et al, 2012;Jiang et al, 2019). The nature of these autotypic junctions is not fully defined, but zebrafish sheaths contain both adherens junctions and desmosomes (Jiang et al, 2019), whereas Drosophila sheaths contain adherens junction (E-cadherin, βcatenin/armadillo) and numerous septate junction proteins (Discs large, Coracle/Band4.1, Nrg/L1CAM, Neurexin-IV, and Scribble, among others; Kim et al, 2012;Tenenbaum et al, 2017;Jiang et al, 2019;Yang et al, 2019). Although the molecular basis for the recruitment of these junctional proteins remains to be determined, studies in Drosophila suggest one plausible mechanism.…”

Section: Developmental Origins Of Ensheathmentmentioning

confidence: 99%

“…Within the nervous system, the EPB41 family protein EPB41L2/4.1G is concentrated at membranes of Schwann cells (Ohno et al, 2006) and plays essential roles in Schwann cell ensheathment, in part through organizing glial transmembrane proteins (Ivanovic et al, 2012;Terada et al, 2019). Similarly, Cora organizes septate junctions (SJs) via interactions with Neurexin-IV and Nrg/L1CAM (Lamb et al, 1998;Ward et al, 1998) and is required for accumulation of Nrg at sheaths (Yang et al, 2019). Epidermal sheaths are enriched in PIP 2 , purified recombinant versions of the Cora FERM domain directly bind PIP 2 (Nunomura et al, 2014), and PIP 2 binding modulates EPB41 family binding specificity for membrane proteins (An et al, 2006).…”

Section: Developmental Origins Of Ensheathmentmentioning

confidence: 99%

See 1 more Smart Citation

Transparent Touch: Insights From Model Systems on Epidermal Control of Somatosensory Innervation

Yin

Peterman

Rasmussen

et al. 2021

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Somatosensory neurons (SSNs) densely innervate our largest organ, the skin, and shape our experience of the world, mediating responses to sensory stimuli including touch, pressure, and temperature. Historically, epidermal contributions to somatosensation, including roles in shaping innervation patterns and responses to sensory stimuli, have been understudied. However, recent work demonstrates that epidermal signals dictate patterns of SSN skin innervation through a variety of mechanisms including targeting afferents to the epidermis, providing instructive cues for branching morphogenesis, growth control and structural stability of neurites, and facilitating neurite-neurite interactions. Here, we focus onstudies conducted in worms (Caenorhabditis elegans), fruit flies (Drosophila melanogaster), and zebrafish (Danio rerio): prominent model systems in which anatomical and genetic analyses have defined fundamental principles by which epidermal cells govern SSN development.

show abstract

Transcriptomic analysis of mosaic brain differentiation underlying complex division of labor in a social insect

Muratore

Mullen

Traniello

2023

J of Comparative Neurology

View full text Add to dashboard Cite

Concerted developmental programming may constrain changes in component structures of the brain, thus limiting the ability of selection to form an adaptive mosaic of size‐variable brain compartments independent of total brain size or body size. Measuring patterns of gene expression underpinning brain scaling in conjunction with anatomical brain atlases can aid in identifying influences of concerted and/or mosaic evolution. Species exhibiting exceptional size and behavioral polyphenisms provide excellent systems to test predictions of brain evolution models by quantifying brain gene expression. We examined patterns of brain gene expression in a remarkably polymorphic and behaviorally complex social insect, the leafcutter ant Atta cephalotes. The majority of significant differential gene expression observed among three morphologically, behaviorally, and neuroanatomically differentiated worker size groups was attributable to body size. However, we also found evidence of differential brain gene expression unexplained by worker morphological variation and transcriptomic analysis identified patterns not linearly correlated with worker size but sometimes mirroring neuropil scaling. Additionally, we identified enriched gene ontology terms associated with nucleic acid regulation, metabolism, neurotransmission, and sensory perception, further supporting a relationship between brain gene expression, brain mosaicism, and worker labor role. These findings demonstrate that differential brain gene expression among polymorphic workers underpins behavioral and neuroanatomical differentiation associated with complex agrarian division of labor in A. cephalotes.

show abstract

Epidermis-Derived L1CAM Homolog Neuroglian Mediates Dendrite Enclosure and Blocks Heteroneuronal Dendrite Bundling

Cited by 14 publications

References 41 publications

An Efficient Screen for Cell-Intrinsic Factors Identifies the Chaperonin CCT and Multiple Conserved Mechanisms as Mediating Dendrite Morphogenesis

An Efficient Screen for Cell-Intrinsic Factors Identifies the Chaperonin CCT and Multiple Conserved Mechanisms as Mediating Dendrite Morphogenesis

Transparent Touch: Insights From Model Systems on Epidermal Control of Somatosensory Innervation

Transcriptomic analysis of mosaic brain differentiation underlying complex division of labor in a social insect

Contact Info

Product

Resources

About