Epidermal growth factor stimulates lactate production and inhibits aromatization in cultured Sertoli cells from immature rats

Mallea, L; Machado, Alberto; Navaroli, Francisco; Rommerts, F. F. G.

doi:10.1111/j.1365-2605.1986.tb00883.x

Cited by 49 publications

(13 citation statements)

References 14 publications

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“…After addition of serum to Sertoli cells this stimulatory effect of FSH on oestradiol production was inhibited [3]. In a previous paper we have shown that EGF may be one of the active principles in serum responsible for this inhibitory effect since EGF inhibits the FSH-stimulated aromatization in Sertoli cells from immature rats whereas insulin had no effect [4].…”

Section: Introductionmentioning

confidence: 99%

“…Sertoli cells were isolated as described by Oonk et al Ill], with slight modifications as reported earlier [4]. Briefly, decapsulated testes from 16-day-old Wistar rats were dispersed twice in phosphate-buffered saline (PBS), containing 0.5 mg/ml collagenase and 20/~g/ml deoxyribonuclease, both times for 30 min at 37°C in a shaking water bath at approx.…”

Section: Isolation and Culture Of Sertoli Cellsmentioning

confidence: 99%

“…Mallea, Instituto Nacional de Endocrinologia, Zapata y D, Vedado, Habana, Cuba by EGF occurred in parallel with the stimulation of lactate production [4]. It is unknown which intracellular mediators are involved in this specific inhibitory effect of EGF on at least one of the many functions of Sertoli cells [5].…”

Section: Introductionmentioning

confidence: 99%

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Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium

Mallea¹,

Machado²,

Navaroli³

et al. 1987

FEBS Letters

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Aromatization of testosterone by cultured Sertoli cells isolated from immature rats was stimulated more than 7-fold by follicle stimulating hormone (FSH) or dcAMP. The effects of FSH and dcAMP could be partly inhibited by epidermal growth factor (EGF) in a dose-dependent manner (IDs0 0.5 nM). The phorbol ester 4fl-phorbol-12-myristate-13-acetate (PMA) could also inhibit aromatase activity in a fashion similar to EGF. When 3 mM EGTA was present in the culture medium, the inhibitory effect of EGF was abolished but the stimulatory effect of FSH or dcAMP was magnified. These results suggest that EGF exerts a negative control on aromatase via calcium and protein kinase C. The abolishment of the inhibitory effect of EGF and the enhancement of the stimulatory effect of FSH or dcAMP by a calcium deficiency may be an indication that growth factors produced by Sertoli cells negatively control FSH-induced responses in an autocrine fashion.

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Section: Introductionmentioning

confidence: 99%

Section: Isolation and Culture Of Sertoli Cellsmentioning

confidence: 99%

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Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium

Mallea¹,

Machado²,

Navaroli³

et al. 1987

FEBS Letters

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“…Several observations have been made on the regulation of lactate production by different locally produced factors. In this regard, EGF (Nehar et al 1993), IL1 (Nehar et al 1998) and TNF in porcine Sertoli cells (Nehar et al 1997) and insulin, insulin-like growth factor-I (Oonk et al 1989), EGF (Mallea et al 1986), PModS (Mullaney et al 1994), FSH and IL1 (Riera et al 2001) in rat Sertoli cells, act as regulators of lactate secretion. A distinct participation of these hormones on the stimulation of the different biochemical steps that will ultimately lead to increased lactate production has been observed.…”

Section: Discussionmentioning

confidence: 99%

Regulation of lactate production and glucose transport as well as of glucose transporter 1 and lactate dehydrogenase A mRNA levels by basic fibroblast growth factor in rat Sertoli cells

Riera

Meroni²,

Schteingart³

et al. 2002

Journal of Endocrinology

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By using cultured rat Sertoli cells as a model, both the action of basic fibroblast growth factor (bFGF) on lactate production and the site of this action were studied. bFGF stimulated Sertoli cell lactate production in a dosedependent manner (basal: 7·3 0·5; 0·1 ng/ml bFGF: 7·5 0·5; 1 ng/ml bFGF: 7·5 0·6; 10 ng/ml bFGF: 10·3 1·0; 30 ng/ml bFGF: 15·2 1·5; 50 ng/ml bFGF: 15·4 1·6 µg/µg DNA). Two major sites for the action of this growth factor were identified. First, bFGF was shown to exert short-and long-term stimulatory effects on glucose transport (basal: 1170 102; 30 ng/ml bFGF for 120 min: 1718 152 and basal: 718 64; 30 ng/ml bFGF for 48 h: 1069 69 d.p.m./µg DNA respectively). Short-term bFGF stimulation of glucose transport was not inhibited by the protein synthesis inhibitor cycloheximide. These results indicate that short-term bFGF stimulation of glucose uptake does not involve an increase in the number of glucose transporters. On the other hand, stimulation with bFGF for periods of time longer than 12 h increased glucose transporter 1 (GLUT1) mRNA levels. These increased mRNA levels were probably ultimately responsible for the increments in glucose uptake that are observed in long-term treated cultures. Secondly, bFGF increased lactate dehydrogenase (LDH) activity (basal: 31·0 1·4; 30 ng/ml bFGF: 45·7 2·4 mIU/µg DNA). The principal subunit component of those LDH isozymes that favors the transformation of pyruvate to lactate is subunit A. bFGF increased LDH A mRNA levels in a dose-and time-dependent manner. In summary, the results presented herein show that glucose transport, LDH activity and GLUT1 and LDH A mRNA levels are regulated by bFGF to achieve an increase in lactate production. These observed regulatory actions provide unequivocal evidence of the participation of bFGF in Sertoli cell lactate production which may be related to normal germ cell development.

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“…En effet, les cellules de la lign~e germinale ne m~taboli-sent pas le glucose mais utilisent pr~f~ren-tiellement le lactate provenant des cellules de Sertoli [54]. La FSH, rinsuline mais aussi des facteurs locaux comme IGF I, EGF augmentent la production de lactate par les cellules de Sertoli [36,45,54,59]. Ces molecules stimulent la production de lactate en favorisant, ~ court terme, la captation de glucose (principalement en augmentant les transporteurs de glucose).…”

Section: B) Action Indirecte Via Les Cellules De Sertoli 9 Par L'inteunclassified

Rôle des facteurs de croissance et cytokines dans le testicule normal et pathologique

Mauduit

Hamamah

Loras

et al. 1997

Androl.

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RESUME L'6tude de la st6rilit6 masculine a trouv6 depuis 4 ans un regain d'int6r6t avec l'av~nement d'une nouvelle technique pour le traitement de rinfertilit6 masculine, l'injection intracytoplasmique d'un spermatozoide dans l'ovocyte (ICSI). N6anmoins, l'6tude des caract6ristiques du sperme demeure le maitre examen dans r6valuation de la fertilit6 masculine. II constitue le reflet de l'activit6 testiculaire et des 6v6nements post-gonadiques, en particulier, la maturation 6pididymaire. Ces processus sont pour la plupart, non seulement sous le contr61e du syst~me neuroendocrinien, mais aussi, sous le contr61e de facteurs locaux tels que les facteurs de croissance et les cytokines. Dans cette revue, nous pr6-sentons, dans une premiere partie, les donn6es montrant le r61e crucial que jouent les facteurs locaux sur la formation du testicule fatal mais aussi sur les fonctions diff6renci6es de la gonade comme la st6roidogen~se et la spermatogen~se. Dans une seconde partie, nous pr6sentons les donn6es obtenues dans le cadre de l'exploration de l'homme infertile, ainsi que les effets in vitro des facteurs locaux sur le pouvoir f6condant des spermatozoides. Ces derni~res donn6es sont encore fragmentaires et l'utilisation des facteurs de croissance et des cytokines dans le domaine des st6rilit6s dites idiopathiques n'en est qu'~ ses d6buts. Enfin, dans le cadre d'une d6marche prospective, nous indiquons quelques voies de recherche qui nous semblent int6res-santes concernant l'6tude des facteurs de croissance et des cytokines dans les st6rilit6 masculines.

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Epidermal growth factor stimulates lactate production and inhibits aromatization in cultured Sertoli cells from immature rats

Cited by 49 publications

References 14 publications

Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium

Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium

Regulation of lactate production and glucose transport as well as of glucose transporter 1 and lactate dehydrogenase A mRNA levels by basic fibroblast growth factor in rat Sertoli cells

Rôle des facteurs de croissance et cytokines dans le testicule normal et pathologique

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