2010
DOI: 10.1016/j.bbagrm.2010.08.004
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Epidermal growth factor regulates PAI-1 expression via activation of the transcription factor Elk-1

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Cited by 18 publications
(13 citation statements)
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“…As mentioned in section 2.5, PAI-1 promoter (pm) 1 contained TATA box, c-Ets, Elk-1, NFkB, and C/EBP; pm2 contained p300 and USF-2; pm3 contained C/EBP and AP-1; and pm4 contained c-Ets, NRF-2 binding site. It has been reported that EGF activates Ets binding site by phosphorylation of Elk1 (49); hypoxia activates C/EBP (24); oxidative stress activates AP-1 (47); EGF receptor activation activates NF-B, steroid receptor coactivator-1 potentiates TGF-␤/Smad signal, facilitating the functional link between Smad3 and p300/CBP (8); and TGF-␤ signal facilitates Smad/p53/USF-2 transcriptional complex (40), resulting in PAI-1 transcription (39). In addition, shRNA knock-down of Nrf2 and loss of Nrf2/Smad3/Smad4 complex resulted in increased transcription of PAI-1.…”
Section: Discussionmentioning
confidence: 99%
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“…As mentioned in section 2.5, PAI-1 promoter (pm) 1 contained TATA box, c-Ets, Elk-1, NFkB, and C/EBP; pm2 contained p300 and USF-2; pm3 contained C/EBP and AP-1; and pm4 contained c-Ets, NRF-2 binding site. It has been reported that EGF activates Ets binding site by phosphorylation of Elk1 (49); hypoxia activates C/EBP (24); oxidative stress activates AP-1 (47); EGF receptor activation activates NF-B, steroid receptor coactivator-1 potentiates TGF-␤/Smad signal, facilitating the functional link between Smad3 and p300/CBP (8); and TGF-␤ signal facilitates Smad/p53/USF-2 transcriptional complex (40), resulting in PAI-1 transcription (39). In addition, shRNA knock-down of Nrf2 and loss of Nrf2/Smad3/Smad4 complex resulted in increased transcription of PAI-1.…”
Section: Discussionmentioning
confidence: 99%
“…We designed the ChIP PCR primers for four randomly segmented regions, which contained the transcription factor binding sites reported by others (24,40,47,49), or oxidative stress or inflammation-related transcription factor binding sites predicted by us. ChIP PCR primer sequences were 5=-AGGCTCGAG-GAAGGGAATTCCAAA-3= and 5=-TGATCCAGCTGTGCTC-CGTT-3= for Ϫ289 to approximately ϩ45 of the PAI-1 promoter (pm) region (PAI-1 pm1) when the TATA site was set as 0; 5=-ACACCAGGAGAGTCTGGCCCATGT-3= and 5=-ACTTCAAGTC-CTTTCCTCCTCCCT-3= for Ϫ596 to approximately Ϫ507 of PAI-1 pm (PAI-1 pm2); 5-=AGCTGCAGATTGAGTTCCAGGCTA-3= and 5=-TTTGTGTCAATAGCCCTTGTGCGG-3= for Ϫ895 to approximately Ϫ611 of PAI-1 pm (PAI-1 pm3); and 5=-AACTTCCATTCCCAACAC-CCACGA-3= and 5=-TTCTGCCTCCTGAGTGCTCGATTA-3= for Ϫ1,130 to approximately Ϫ930 of PAI-1 pm (PAI-1 pm4) sense and antisense, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…These data indicate that PAI‐1 may be upstream of EGF and EGF receptor signaling. EGF‐treated breast adenocarcinoma and glioma cells rapidly up‐regulate PAI‐1 expression (32, 33). TGF‐β–induced PAI‐1 expression requires EGF receptor–mediated signaling (34).…”
Section: Discussionmentioning
confidence: 99%
“…Elk-1 belongs to the ternary complex factor subfamily, which is a subgroup of the Ets family of transcription factors. 35, 36 Elk-1 participates in the regulation of many important genes, such as c-fos, 37 plasminogen activator inhibitor-1, 38 EZH2, 39 Mcl-1, 40 PKC alpha 41 and cyclin D1, 42 which are involved in the regulation of cell proliferation and apoptosis. Elk-1 is associated with tumorigenesis of many different malignant diseases, such as cancer of the breast, 42, 43 colon, 44 endometrium 45 and HCC.…”
Section: Discussionmentioning
confidence: 99%