Epidermal Growth Factor Receptors on Corneal Endothelium

Fabricant, Robert; Alpar, Andrew J; Centifanto, Ysolina M.; Kaufman, Herbert E.

doi:10.1001/archopht.1981.03930010307019

Cited by 50 publications

(17 citation statements)

References 10 publications

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“…In normal mice, whose tears contained higher concentrations of EGF-like substance, the effect of exogeneously applied EGF was not apparent. In addition, it has been shown that the cornea possesses EGF receptors (14,15) and EGF stimulates proliferation of epithelial and endothelial cells ofthe cornea in culture (5, 6). These results are also consistent with the view that EGF plays a role in the corneal regeneration.…”

Section: Discussionmentioning

confidence: 50%

Epidermal growth factor-like, corneal wound healing substance in mouse tears.

Tsutsumi¹,

Tsutsumi²,

Oka³

1988

J. Clin. Invest.

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We have identified the presence of a putative corneal wound healing substance in mouse tears, which has a molecular size and immunological properties similar to those of epidermal growth factor (EGF). The substance was capable of binding to EGF receptors in mouse parenchymal cells and this binding was inhibited by anti-EGF serum. The concentration of the EGF-like substance in the tears of male and female mice was estimated to be 79.3±7.0 (SD) ng/ml and 76.5±8.1 (SD) ng/ml, respectively, by EGF radioimmunoassay. Removal of the submandibular glands, which produce large amounts of EGF, reduced plasma EGF to an undetectable level and also decreased the concentration of the EGF-like substance in tears to 27.3±3.9 (SD) ng/ml in male mice and 25.8±3.7 (SD) ng/ml in female mice. Approximately 50% of sialoadenectomized (submandibular glands removed) male mice with deep corneal wounds developed severe ocular lesions or loss of sight whereas none of normal male mice with similar wounds did. Topical application of EGF to deeply wounded eyes of sialoadenectomized mice eliminated the various complications and restored the healing rate and incidence of recovery to virtually normal levels.

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Section: Discussionmentioning

confidence: 50%

Epidermal growth factor-like, corneal wound healing substance in mouse tears.

Tsutsumi¹,

Tsutsumi²,

Oka³

1988

J. Clin. Invest.

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“…The regenera tive capacity of corneal endothelium is, known to be very limited in primates [1], However, recent studies have given evidence that also corneal endothelial cells are capable of mitotic activity during wound healing [2]. This is in accordance with studies showing enhanced endothelial cell density after appli cation of epidermal growth factor (EGF) [3,4], The exposure of corneal endothelial cells to EGF could furthermore be associated with the stimulation of DNA synthesis [5], Mole cules like EGF could hence also enhance ex tracellular matrix proteins synthesis by endo thelial cells similarly as it has been shown for cultured fibroblasts [6] and for corneal tissue [7] , The control mechanisms for such a stimu lation, however, seem to differ between epi thelial cells, keratocytes and endothelial cells [8] and are still less understood. In spite of the demonstrated physiological absence of EGF in anterior chamber fluid (ACF) [9], the pres ence of EGF in ACF is still subject to discus sion [10,11].…”

Section: Introductionsupporting

confidence: 39%

Growth Factors and Their Receptors in the Anterior Chamber

Setten¹,

Philipson²,

Schultz³

1996

Ophthalmic Res

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The involvement of growth factors such as epidermal growth factor (EGF) in regenerative processes of corneal endothelium and lens epithelium has recently been suggested. However, knowledge on the presence of growth factors in anterior chamber fluid (ACF) is still very restricted. Although we have previously shown that EGF is undetectable in the ACF of normal eyes undergoing cataract surgery even by the use of high-sensitivity assays, this does not exclude the possible presence of other, EGF-like proteins in ACF such as transforming growth factor α (TGF-α). In the present study, we have hence determined in ACF samples of 70 human eyes the concentrations of both EGF and TGF-α. As assays served ELISA techniques and RIA. In none of all the samples investigated could detectable amounts of EGF, i.e. above 0.2 pg/ml (detection limit of the assay), be found, confirming earlier results. Interestingly, however, also no TGF-α could be detected in ACF. If present at all, the level of any TGF-α concentration in ACF was hence below the detection limit, i.e. less than 20 pg/ml. Based on the results of this study, it seems therefore that under physiological conditions there is no measurable presence of free EGF or TGF-α in human ACF. Existing receptors in the structures of the anterior segment must hence have ample binding capacity which could explain the effect of externally applied growth factors. The physiological and clinical importance of this result is briefly outlined.

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“…In partic ular, EGF eye drops have been shown to re duce the healing times after viral or traumatic corneal ulcers or erosions [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 45%

Epidermal Growth Factor in Topical Treatment following Epikeratoplasty

Caporossi¹,

Manetti²

1992

Ophthalmologica

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The efficacy of epidermal growth factor (EGF) in reducing the healing time of the cornea after an epikeratoplasty has been evaluated in an open study in two groups of patients. The time required for complete reepithelialization of the cornea was recorded, and the data obtained were analyzed statistically. In the EGF group the reepithelialization was significantly faster than in the control group. These results indicate that EGF is effective in reducing the reepithelialization time of the cornea after an epikeratoplasty.

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Epidermal Growth Factor Receptors on Corneal Endothelium

Cited by 50 publications

References 10 publications

Epidermal growth factor-like, corneal wound healing substance in mouse tears.

Epidermal growth factor-like, corneal wound healing substance in mouse tears.

Growth Factors and Their Receptors in the Anterior Chamber

Epidermal Growth Factor in Topical Treatment following Epikeratoplasty

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