Epidermal growth factor receptor vIII enhances tumorigenicity and resistance to 5-fluorouracil in human hepatocellular carcinoma

Mutual Regulation of Receptor-Mediated Cell Signalling and Endocytosis: EGF Receptor System as an Example

“…In spite of not binding any ligands, the receptor is constitutively active [146], and is able to activate Ras-Erk1/2 and PI 3-kinase-Akt pathways [142,147]. In concordance with this, EGFRvIII was shown to transform fibroblasts and to enhance the proliferation and/or tumorigenicity of cells both in vivo and in vitro [142,[148][149][150][151][152]. The constitutive activity may be important for tumorigenicity, but impaired downregulation certainly enhances the effect.…”

Bioprocess development for the production of mouse-human chimeric anti-epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells

“…The recombinant CHO-C12 cell lines were established by transfecting the chimeric anti-EGFRvIII C12 antibody expression vector into CHO-DG44 cells, as described in our previous work (Wang 2009). Stable recombinant cells were selected in Dulbecco's Modified Eagle Medium (DMEM), containing 7% fetal bovine serum (FBS) and 400 nM methotrexate (MTX) and adapted to suspension culture in shaker flasks.…”

“…The level of EGFRvIII expression was closely correlated with increased tumorigenicity in mouse models (Nishikawa et al 1994;Ramnarain et al 2006) and poor prognosis of brain cancer in clinical settings (Diedrich et al 1995;Liu et al 2005). In addition, the EGFRvIII mutation has been detected in HCC tissue samples (Ou et al 2005) and found to play a vital role in tumorigenicity and enhanced resistance to 5-Fluorouracil (5-FU) treatment of HCC (Wang et al 2009). Therefore, in our previous work, we successfully developed and produced the chimeric anti-EGFRvIII antibody C12 from CHO-DG44 cells.…”

“…Therefore, in our previous work, we successfully developed and produced the chimeric anti-EGFRvIII antibody C12 from CHO-DG44 cells. This antibody binds to the same sites, possesses similar affinity to ligand as the monoclonal antibody 12H23, and effectively reduces the human anti-mouse antibody response (HAMA) (Wang 2009). Thus, there is an increasing demand for purified C12 antibody in large amounts for use in pre-clinical or clinical studies or in studies on its biological character.…”

Single Chain Fragment Variables Antibody binding to EGF Receptor in the Surface of MCF7 Breast Cancer Cell Line: Application and Production Review

“…Which are constructed using phage display technology and molecular cloning and the specific bio-panning selection with the use of the epidermal growth factor receptor (EGFR) at the surface of MCF-7 cells as the antigen for the straightforward specific selection are considered as fast and accurate way of producing specific antibodies [3]. In this review, the process of constructing a single chain antibody by using phage display technologies, which are sequence of known experiments, that in the end produce high specific antibodies described in detail.…”