Epidermal growth factor receptor expression by human squamous cell carcinomas of the head and neck, cell lines and xenografts

Stanton, Peter; Richards, Sean; Reeves, Jonathon R.; Nikolić, Milan; Edington, Kirsten G.; Clark, Louise; Robertson, Gerry; Souter, D; Mitchell, Rod T; Hendler, F. J.; Cooke, Timothy G.; Parkinson, E.K.; Ozanne, Brad

doi:10.1038/bjc.1994.322

Cited by 72 publications

(58 citation statements)

References 35 publications

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“…As a human model epidermoid carcinoma cell line, A431 has been widely used in studies on the cell cycle and tumor related cell signaling pathways because epidermal growth factor receptor (EGFR) is known to be strikingly upregulated in these cells (65)(66)(67)(68). In this study, we found that in the A431 cell, overexpression of PDCD5 inhibits cell proliferation, induces cell cycle arrest at G2/M phase and apoptosis.…”

Section: Discussionmentioning

confidence: 54%

PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

Hong-xin

Wang

et al. 2017

Oncol Lett

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Abstract. Programmed cell death (PDCD)5 is cloned from human leukemia cell line TF-1. PDCD5 is one of the members of the programmed cell death protein family that is frequently involved in tumor growth and apoptosis. To investigate the molecular and cellular functions of PDCD5, the present study established a PDCD5 stably overexpressing A431 cell line and examined the role of PDCD5 in cell proliferation, cell cycle progression and apoptosis. The data demonstrated that overexpression of PDCD5 significantly inhibited cell proliferation, induced cell cycle arrest at G2/M phase and apoptosis in A431 cells. The expression profiles of certain key regulators of these cellular events were further investigated, including P53, B cell lymphoma (BCL)-2, BCL-2 associated X protein (BAX) and caspase (CASP)3. The data demonstrated that at the transcript and protein levels, P53, BAX and CASP3 were all upregulated in the PDCD5 stably overexpressing A431 cells whereas BCL-2 was downregulated, indicating that PDCD5 acts as an important upstream regulator of P53, BCL-2, BAX and CASP3. The data suggest that PDCD5 regulates cell proliferation, cell cycle progression and apoptosis in A431 cells. PDCD5 may be a novel tumor suppressor gene, and may be potentially used for cancer treatment in the future.

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Section: Discussionmentioning

confidence: 54%

PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

Hong-xin

Wang

et al. 2017

Oncol Lett

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“…To address this question, we introduced a single normal copy of human chromosome 4 into two SCC-HN lines that possessed LOH on 4q, BICR6 and BICR31, and two cell lines that did not, BICR3 and BICR19. These cell lines are otherwise matched for p53 (Loughran et al, 1997), p16 INK4A (Loughran et al, 1997), Ras (Clark et al, 1993), epidermal growth factor receptor (Stanton et al, 1994) and chromosome 3 (Loughran et al, 1997) status (Table 1). All the lines are telomerase positive (Loughran et al, 1997).…”

Section: Human Chromosome 4 Specifically Restores Mortality To Scc-hnmentioning

confidence: 99%

Functional evidence for a squamous cell carcinoma mortality gene(s) on human chromosome 4

et al. 2002

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“…Although TGF-a expression has also been detected in normal colon (Malden et al, 1989), co-expression of TGF-a and EGFR and growth stimulation by TGF-a has been demonstrated in multiple colon cancer cell lines (Mulder and Brattain, 1989). Overexpression of EGFR has been reported in squamous cell carcinoma of the skin, oesophagus (Itakura et al, 1994;Stanton et al, 1994), non-small-cell lung carcinoma (Rusch et al, 1993), breast adenocarcinoma and endometrial adenocarcinoma (Khalifa et al, 1994;Miller et al, 1994). In colon cancer it has been well documented that overexpression of EGFR may indicate an advanced stage of the disease (Gross et al, 1991) and may predict the metastatic potential (Radinsky et al, 1995).…”

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confidence: 99%

Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells

Ellinger²,

Sheinin³

et al. 1998

Br J Cancer

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Summary In situ hybridization on human colon tissue demonstrates that epidermal growth factor receptor (EGFR) mRNA expression is strongly increased during tumour progression. To obtain test systems to evaluate the relevance of growth factor action during carcinogenesis, primary cultures from human colorectal carcinomas were established. EGFR distribution was determined in 2 of the 27 primary cultures and was compared with that in well-defined subclones derived from the Caco-2 cell line, which has the unique property to differentiate spontaneously in vitro in a manner similar to normal enterocytes. The primary carcinoma-derived cells had up to three-fold higher total EGFR levels than the Caco-2 subclones and a basal mitotic rate at least fourfold higher. The EGFR affinity constant is 0.26 nmol 1-1, which is similar to that reported in Caco-2 cells. The proliferation rate of Caco-2 cells is mainly induced by EGF from the basolateral cell surface where the majority of receptors are located, whereas primary cultures are strongly stimulated from the apical side also. This corresponds to a three-to fivefold higher level of EGFR at the apical cell surface. This redistribution of EGFR to apical plasma membranes in advanced colon carcinoma cells suggests that autocrine growth factors in the colon lumen may play a significant role during tumour progression.

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Epidermal growth factor receptor expression by human squamous cell carcinomas of the head and neck, cell lines and xenografts

Abstract: S_mmary Epidermal growth factor receptor (EGFR) overexpression has been associated frequently with squamous cell carcinomas (SCC) and SCC cell lines. In most cases the level of EGFR on the tumours from which the cell lines were derived has not been determined, nor

Cited by 72 publications

References 35 publications

PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

PDCD5 regulates cell proliferation, cell cycle progression and apoptosi

Functional evidence for a squamous cell carcinoma mortality gene(s) on human chromosome 4

Epidermal growth factor receptor expression in primary cultured human colorectal carcinoma cells

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