Epidermal growth factor receptor activity is necessary for mouse basal cell proliferation

Brechbuhl, Heather M.; Li, Bilan; Smith, R. H.; Reynolds, Susan D.

doi:10.1152/ajplung.00201.2014

Cited by 25 publications

(27 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies reported that ErbB1–ErbB4 and their ligands, including EGF, AREG, and NRG activity, are required for airway epithelial cell proliferation and repair (Britsch, ; Dammann et al, ; Fischer et al, ; Lemjabbar et al, ; Nielsen, ; O'Donnell et al, ; in rewieved Brechbuhl et al, ; Finigan et al, ; Manzo et al, ). Therefore, the presence of EGFRs, together with their ligands, in the basal and ciliated cells of bronchial epithelium in the quail lung throughout the post‐hatching period support the idea that ErbB receptors and ligands regulate tissue homeostasis and stem/progenitor cells (Brechbuhl et al, ; Puddicombe et al, ; Shaykhiev et al ).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of epidermal growth factor system in the lung of the Japanese quail (Coturnix coturnix japonica) during the post-hatching period

Alan

Lïman

Sağsöz

2015

Microsc. Res. Tech.

View full text Add to dashboard Cite

The purpose of this study is to determine the possible changes in the localization of the four Epidermal Growth Factor Receptors and three ligands in quail lungs from the first day of hatching until the 125th after hatching using immunohistochemical methods. Immunohistochemical results demonstrated that four EGFRs and their ligands are chiefly located in the cytoplasm of cells. Additionally, ErbB4, AREG, and NRG1 are localized to the nucleus and nucleolus, but EGF is present in the nucleolus. ErbB2 was also found in the cell membrane. In the epithelium of secondary bronchi, the goblet cells only exhibited ErbB1 and ErbB2, whereas the basal and ciliated cells exhibited EGFRs and ligands immunoreactivity. The atrial granular cells displayed moderate levels of ErbB1-ErbB3 and EGF and strong levels of ErbB4, AREG, and NRG1 immunoreactivity. While the squamous atrial cells and squamous respiratory cells of air capillaries and endothelial cells of blood capillaries exhibited moderate to strong ErbB2, ErbB4, AREG, and NRG1 immunoreactivity, they had negative or weak ErbB1, ErbB3, and EGF immunoreactivity. The expression levels of ErbB2-ErbB4, EGF, AREG, and NRG1 were also detected in fibroblasts. Although ErbB2 was highly expressed in the bronchial and vascular smooth muscle cells, weak expression of ErbB1, ErbB3, AREG and EGF and moderate expression of ErbB4 and NRG1 were observed. Macrophages were only negative for ErbB1. In conclusion, these data indicate that the EGFR-system is functionally active at hatching, which supports the hypothesis that the members of EGFR-system play several cell-specific roles in quail lung growth after hatching.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of epidermal growth factor system in the lung of the Japanese quail (Coturnix coturnix japonica) during the post-hatching period

Alan

Lïman

Sağsöz

2015

Microsc. Res. Tech.

View full text Add to dashboard Cite

show abstract

“…However, this idea has not yet been assessed in postnatal lung maturation. This study, along with the work of Brechbuhl and coworkers (48), adds the IGF system and the EGF system to the cocktail of modulators of basal cell differentiation and proliferation.…”

Section: Growth Factorsmentioning

confidence: 99%

Recent advances in the mechanisms of lung alveolarization and the pathogenesis of bronchopulmonary dysplasia

Silva

Nardiello

Pozarska

et al. 2015

American Journal of Physiology-Lung Cellular and Molecular Phys

120

117

View full text Add to dashboard Cite

Alveolarization is the process by which the alveoli, the principal gas exchange units of the lung, are formed. Along with the maturation of the pulmonary vasculature, alveolarization is the objective of late lung development. The terminal airspaces that were formed during early lung development are divided by the process of secondary septation, progressively generating an increasing number of alveoli that are of smaller size, which substantially increases the surface area over which gas exchange can take place. Disturbances to alveolarization occur in bronchopulmonary dysplasia (BPD), which can be complicated by perturbations to the pulmonary vasculature that are associated with the development of pulmonary hypertension. Disturbances to lung development may also occur in persistent pulmonary hypertension of the newborn in term newborn infants, as well as in patients with congenital diaphragmatic hernia. These disturbances can lead to the formation of lungs with fewer and larger alveoli and a dysmorphic pulmonary vasculature. Consequently, affected lungs exhibit a reduced capacity for gas exchange, with important implications for morbidity and mortality in the immediate postnatal period and respiratory health consequences that may persist into adulthood. It is the objective of this Perspectives article to update the reader about recent developments in our understanding of the molecular mechanisms of alveolarization and the pathogenesis of BPD.

show abstract

“…It was reported that HGF stimulated AEC II cell proliferation and induced lung tissue regeneration in COPD models (50,51). EGF was reported necessary for mouse epithelial cell proliferation and repair (52). SLPI can inhibit protease and consequently reduce the level of elastin-breakdown by protease in lung parenchyma (16).…”

Section: Pre-clinical Results With the Infusion Of Mscsmentioning

confidence: 99%

Stem cell therapies for chronic obstructive pulmonary disease: current status of pre-clinical studies and clinical trials

Sun¹,

Li²,

Zhou³

et al. 2018

J. Thorac. Dis.

View full text Add to dashboard Cite

Chronic obstructive pulmonary disease (COPD) is a respiratory disease that has a major impact worldwide. The currently-available drugs mainly focus on relieving the symptoms of COPD patients. Novel regenerative therapeutic approaches have been investigated with the aim of repairing or replacing the injured functional structures of the respiratory system. We summarized the progress made by regenerative therapies for COPD by analyzing results from both pre-clinical studies and completed clinical trials. These approaches include the application of exogenous stem cells or small molecules to stimulate the regeneration by endogenous lung stem/progenitor cells. Exogenous mesenchymal stem cells (MSCs) have been reported to repair the structure and improve the function of the injured respiratory system in COPD models. However, the studies that used MSCs in patients with moderate-to-severe COPD patients did not lead to clear respiratory functional improvements. Exogenous human lung stem cells applied to cryo-injured (CI) lungs of mice have been shown to organize into human-like pulmonary structures, indicating a new property of stem cells that is potentially capable of curing COPD patients. Small molecules like retinoic acid has been shown to lead to regeneration and repair of the damaged lung structures in COPD mouse models probably by activation of endogenous lung stem/progenitor cells. However, retinoic acid or agonists of retinoic acid receptor administered to moderate or severe COPD patients did not improve the density and function of the damaged lung. These novel regenerative approaches have failed in preliminary clinical trials, possibly due to the advanced severity of the disease. Further work should be done to develop the current regenerative approaches for curing patients at different stages of COPD. We suggest that some modifications of the approach in the clinical studies may lead to more successful outcomes of regenerative therapy for COPD.

show abstract

Epidermal growth factor receptor activity is necessary for mouse basal cell proliferation

Cited by 25 publications

References 56 publications

Immunohistochemical localization of epidermal growth factor system in the lung of the Japanese quail (Coturnix coturnix japonica) during the post-hatching period

Immunohistochemical localization of epidermal growth factor system in the lung of the Japanese quail (Coturnix coturnix japonica) during the post-hatching period

Recent advances in the mechanisms of lung alveolarization and the pathogenesis of bronchopulmonary dysplasia

Stem cell therapies for chronic obstructive pulmonary disease: current status of pre-clinical studies and clinical trials

Contact Info

Product

Resources

About