Epidermal Growth Factor Promotes Epidermal Growth Factor Receptor Nuclear Accumulation by a Pathway Dependent on Cytoskeleton Integrity in Human Breast Cancer Cells

Cortés‐Reynosa, Pedro; Robledo, Teresa; Salazar, Eduardo Pérez

doi:10.1016/j.arcmed.2009.06.007

Cited by 7 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current work provides evidence that dynamin and clathrin are necessary first steps for translocation of EGFR to the nucleus. It has been reported that treatment with colchicine and cytochalasin D inhibited the nuclear accumulation of EGFR [33]. In contrast, other report showed that ErbB3 nuclear translocation is clathrin-independent in prostate cancer cell lines [34].…”

Section: Discussionmentioning

confidence: 99%

Epidermal growth factor receptors destined for the nucleus are internalized via a clathrin-dependent pathway

Campos

Rodrigues

Andrade

et al. 2011

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The epidermal growth factor (EGF) transduces its actions via the EGF receptor (EGFR), which can traffic from the plasma membrane to either the cytoplasm or the nucleus. However, the mechanism by which EGFR reaches the nucleus is unclear. To investigate these questions, liver cells were analyzed by immunoblot of cell fractions, confocal immunofluorescence and real time confocal imaging. Cell fractionation studies showed that EGFR was detectable in the nucleus after EGF stimulation with a peak in nuclear receptor after 10 min. Movement of EGFR to the nucleus was confirmed by confocal immunofluorescence and labeled EGF moved with the receptor to the nucleus. Small interference RNA (siRNA) was used to knockdown clathrin in order to assess the first endocytic steps of EGFR nuclear translocation in liver cells. A mutant dynamin (dynamin K44A) was also used to determine the pathways for this traffic. Movement of labeled EGF or EGFR to the nucleus depended upon dynamin and clathrin. This identifies the pathway that mediates the first steps for EGFR nuclear translocation in liver cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Epidermal growth factor receptors destined for the nucleus are internalized via a clathrin-dependent pathway

Campos

Rodrigues

Andrade

et al. 2011

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…Epithelial growth factor receptor (EGFR) is one of these proteins that are significantly upregulated on the cell surface on malignant cells. [47] Recent evidence in the literature suggests that EGFR and similar receptors are present within the nucleus of the normal cell. Multiple membrane-bound receptor tyrosine kinases (RTKs), such as the epidermal growth factor receptor and ErbB-2, have been reported to be localized in the nucleus, and emerging evidence suggests that they are involved in transcriptional regulation, cell proliferation, DNA repair, and chemo and radio resistance.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that tumor cells greatly upregulate the production of several surface proteins. Epithelial growth factor receptor (EGFR) is one of these proteins that are significantly upregulated on the cell surface on malignant cells 47. Recent evidence in the literature suggests that EGFR and similar receptors are present within the nucleus of the normal cell.…”

Section: Discussionmentioning

confidence: 99%

Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity

Guze

Short

Zeng

et al. 2010

J Raman Spectroscopy

View full text Add to dashboard Cite

Raman spectroscopy has been effectively applied to clinically differentiate normal and cancerous mucosal tissues. Micro-Raman spectroscopy provides a tool to better understand the molecular basis for the Raman clinical signal. The objective of the current study was to utilize micro-Raman spectroscopy to define the molecular/spectral differences between normal and abnormal squamous cell carcinoma (SCC) in oral mucosa (in vitro). Understanding this may help in identifying unique spectra or may be useful for in vivo application of this technology. Micro-Raman (confocal) spectroscopy was used to obtain molecular images of normal and SCC cells of human oral mucosa. Four fresh flashed-frozen tumor and four matched normal tongue specimens were studied. The spectra covered a wavenumber range from 300 to 4000 cm −1 with a spectral resolution of 8 cm −1 and a spatial resolution of 1.0 µm. The cells were located within thin sections of tongue mucosa biopsies. The excitation wavelength of 515 nm was used. We were able to obtain Raman images with rich information about the spectroscopic and structural features within the cytoplasm, cell membrane, and cell nuclei. Significant spectral differences were observed between the Raman images of normal and malignant squamous cells. The heterogeneity of tumor cells within the abnormal tissue was also demonstrated. Spectral differences demonstrated between both tissue types have provided important information regarding the origins of specific signals within the cells of each tissue type. In our search for specific spectral biomarkers, we believe that a cell surface protein, greatly upregulated in SCC cells, was discovered at 1583 cm −1 .

show abstract

“…Many integral membrane proteins have been previously reported to be translocated to the nucleus through many hypothetical mechanisms and the biological consequences of this translocation are currently under investigation. Regarding the nuclear trafficking of EGFR family proteins, endocytosis and endosomal sorting are both required for the nuclear transport of the EGFR complex (Cortes-Reynosa et al, 2009). However, the detailed mechanism by which the internalized EGFR proteins are routed to the nucleus through the NPC is still unclear (Wanget al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Morel¹

2023

Preprint

View full text Add to dashboard Cite

Growth hormone (GH) and many other hormones or growth factors have been shown to be rapidly internalized and translocated into the nucleus. The first event of peptide action is binding to its receptor which initiates both signal transduction pathways and internalization. The latter process involves the nucleus and probably gene transcription. In order to analyze the consequences of internalization of GH on gene expression, we used different populations of CHO cells, transfected with either full length GH receptor, or with defective receptor unable to trigger either signal transduction (deletion of box1) or the internalization of GH (Phe346mutation in Ala). In addition, functional isolated nuclei were incubated 10 and 30 min with 50 nM GH in order to analyze the direct effect of GH on gene expression without surrounding cytoplasmic structures. The genes involved in signal transduction pathways were not revealed if GH internalization is the only functional activity in the whole cell as well as with isolated nuclei. In intact cell, internalization increased expression of 297 genes and decreased fewer than 10% of those known to be influenced by GH. Variations of expression in purified nuclei showed large variations with time. If cell signaling was not modified, cellular growth and proliferation, nucleic acid metabolism, cellular development, cell cycle and gene expression showed many variations with time. GH internalization shows direct effects on gene expression, different from those stimulated by signal transduction.

show abstract

Epidermal Growth Factor Promotes Epidermal Growth Factor Receptor Nuclear Accumulation by a Pathway Dependent on Cytoskeleton Integrity in Human Breast Cancer Cells

Cited by 7 publications

References 45 publications

Epidermal growth factor receptors destined for the nucleus are internalized via a clathrin-dependent pathway

Epidermal growth factor receptors destined for the nucleus are internalized via a clathrin-dependent pathway

Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Contact Info

Product

Resources

About