Epidermal growth factor promotes chick embryonic angiogenesis

Stewart, R. H.; Nelson, Jane; Wilson, David J.

doi:10.1016/0309-1651(89)90079-9

Cited by 28 publications

(11 citation statements)

References 12 publications

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“…To our knowl edge it has not been shown so far that these factors stimu late growth and vascularization of the yolk sac membrane. The proliferative effect of epidermal growth factor shown by Stewart et al [21] may not be confined to the vascular endothelium, but seems to stimulate the development of ecto-, meso-and endoderm of the area vasculosa. In the meantime it has been shown that VEGF may play a role in vasculogenesis and angiogenesis [26].…”

Section: Discussionmentioning

confidence: 82%

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Influence of Environmental Oxygen Concentration on Growth and Vascular Density of the Area vasculosa in Chick Embryos

Höper

Jahn

1995

Int J Microcirc

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The influence of different environmental oxygen concentrations on vascularization was studied in the yolk sac blood vessel system of chick embryos. The eggs were incubated at normoxic (20.9% oxygen, n = 29), hypoxic (10% oxygen, n = 36) or hyperoxic (100% oxygen, n = 26) conditions and evaluated on day 4 of incubation. In vivo photographs were taken of the vascularized part of the yolk sac membrane (area vasculosa). Prints of a given enlargement were evaluated by histometrical methods to measure the extension of the area vasculosa, the density and the total area of blood vessels. Hypoxia induced an increase in vascular density, in area occupied by blood vessels (44.4%, normoxia = 40.6%) and an enlargement of the area vasculosa (441 ± 78.6 mm2, normoxia = 380 ± 68.7 mm2). Hyperoxia also induced an increase in vascular density and in the area occupied by blood vessels (45.9%), but a decline in the extension of the vascularized area (287 ± 48.8 mm2). Thus the total number of blood vesels as well as the total exchange surface decrease in hyperoxia. Our results indicate that in the area vasculosa of the early chick embryo hypoxia possibly causes an upregulation of vascular growth factors. From the present results it cannot be decided whether vasculogenesis or angiogenesis is stimulated.

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Section: Discussionmentioning

confidence: 82%

“…VEGF is not the only factor known to stimulate vasculogenesis and angiogenesis. Stewart et al [21] showed that epidermal growth factor promotes extraembryonic angio genesis in the area vasculosa of 3-day-old chicks.…”

Section: Discussionmentioning

confidence: 99%

Influence of Environmental Oxygen Concentration on Growth and Vascular Density of the Area vasculosa in Chick Embryos

Höper

Jahn

1995

Int J Microcirc

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“…We have used the chick embryo area vasculosa in shell-less culture as our experimental model. This model has been well validated for studies on blood vessel development (Stewart et al 1989 ;Allen & Wilson, 1993) and has the advantage of allowing direct and continuous observation of all stages of vascular development in the same embryo (Flamme et al 1993).…”

Section: mentioning

confidence: 99%

The effects of lithium on vascular development in the chick area vasculosa

Giles

Bannigan

1999

Journal of Anatomy

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The effects of lithium on vascular development were examined using the chick embryo area vasculosa in shell-less culture as an experimental model. Embryos were explanted after 48 h in ovo and LiCl (50, 100, 150 and 200 µg in 10 µl water) was applied to the centre of the blastodisc. Controls were untreated or given equimolar amounts of NaCl. At 24 h and 48 h after treatment, untreated and NaCl controls were identical, having well developed extraembryonic vessels. At doses of 100 µg and greater, LiCl significantly inhibited normal vascular development and expansion of the area vasculosa in the majority of explants. In many specimens blood islands continued to form but their assembly into primitive vessels was prevented, indicating that lithium affects the mechanism regulating the assembly of vascular endothelium. At the same time the embryos were alive but retarded in development compared with controls. When LiCl (150 µg) was applied to cultures explanted after 72 h in ovo (when the primary vascular network had already formed through vasculogenesis) no adverse effects were seen. This suggests that lithium affects vasculogenesis but not angiogenesis. Treatment with myo-inositol completely reversed the effects of lithium in a time dependent manner indicating that the phosphatidylinositol second messenger cycle may be involved in the cellular events of vasculogenesis. Finally the results of this study show that the yolk sac vasculature is particularly vulnerable to lithium and the consequent effects of this interference on embryonic development are discussed.

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“…Elvax slabs or films have been used to control the release of angiogenic factors in vivo (Gospodarowicz et al, 1979;Silberstein et al, 1987;Fajardo et al, 1988) and in vitro (Murray et al, 19831, and to deliver antigens for immunization (Langer, 1981). Commercially available sponges made of gelatin or collagen and Millipore filter discs impregnated with growth factors have been used in in vivo (Thompson et al, 1984;Lindenbaum et al, 1989) and in vitro (Stewart et al, 1989) studies of angiogenesis as well. These systems have the C 1992 WILEY-LISS, INC drawback that they must be surgically implanted in experimental animals.…”

mentioning

confidence: 99%

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

Downs¹,

Robertson²,

Riss³

et al. 1992

Journal Cellular Physiology

140

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A method previously used in this laboratory for entrapment of tumor cells in alginate beads has been extended to provide a slow release delivery system for growth factors with known in vivo angiogenic activity. Protein growth factors were entrapped in alginate beads in amounts sufficient to cause incorporation of 3H-thymidine by COMMA-D cells in vitro, and in vivo neovascularization when injected subcutaneously into Balb/c mice. Entrapment of 125I-labelled growth factors showed that the amount of molecule entrapped in alginate beads may vary with the charge of the molecule. In vitro cell proliferation studies showed that entrapment in alginate beads may provide a slow-release system or a stabilizing environment for the protein. In some cases biological activity of the growth factor in solution was increased by the presence of control alginate beads. When alginate-entrapped growth factors were injected into Balb/c mice, induction of new blood vessels could be monitored qualitatively by macroscopic photography and assessed quantitatively by measuring the pooling of radiolabelled red blood cells at the experimental site. Subcutaneous injection of purified angiogenic factors not entrapped in alginate beads did not cause neovascularization. Diffusion of 125I-labelled growth factors from alginate beads in the animal showed that release in vivo may depend on the charge of the protein molecule. These results indicate that injection of purified molecules entrapped in alginate beads provides an effective localized and slow-release delivery of biologically active molecules. This delivery system may extend the time of effectiveness of biologically active molecules in vivo compared to direct injection without alginate entrapment. The method of entrapment and injection has potential for identifying active factors in tumor-induced angiogenesis and testing new compounds as modulators of neovascularization.

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Epidermal growth factor promotes chick embryonic angiogenesis

Cited by 28 publications

References 12 publications

Influence of Environmental Oxygen Concentration on Growth and Vascular Density of the Area vasculosa in Chick Embryos

Influence of Environmental Oxygen Concentration on Growth and Vascular Density of the Area vasculosa in Chick Embryos

The effects of lithium on vascular development in the chick area vasculosa

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

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