Epidermal Growth Factor Prevents Acetaldehyde‐Induced Paracellular Permeability in Caco‐2 Cell Monolayer

Abstract

These results indicate that EGF attenuates acetaldehyde-induced disruption of tight junctions and adherens junctions and prevents acetaldehyde-induced reorganization of actin cytoskeleton and its interaction with occludin, ZO-1, E-cadherin, and beta-catenin.

Alcohol, intestinal bacterial growth, intestinal permeability to endotoxin, and medical consequences: Summary of a symposium

“…The importance of ethanol metabolism into acetaldehyde was further confirmed when acetaldehyde, and not ethanol, was shown to increase mucosal permeability in proximal rat colonic strips mounted to Ussing chambers in vitro (Ferrier et al, 2006). Further mechanistic studies showed that acetaldehyde-induced increase in paracellular permeability is associated with redistribution of tight junction proteins (occludin and ZO1) and adherens junction (E-cadherin and β-catenin) proteins from the intercellular junctions into the intracellular compartments (Atkinson and Rao, 2001;Seth et al, 2004;Sheth et al, 2004).…”

Editorial - Augmenting Epithelial Barrier Function

“…Some agents, e.g. Epidermal Growth Factor and glucorticoids, [12][13][14][15][16] While this may not necessarily be good for intellectual property rights, it could be quite a boon for the average individual who needs inexpensive and readily-administered therapeutic interventions. Each one of these agents has its own anecdotal (or better) 'resume' attesting to beneficial health effects.…”

Role of Extracellular Signal-Regulated Kinase (ERK) in Regulation of Intestinal Epithelial Tight Junctions

“…28,94,[144][145][146][147][148][149][150][151][152][153][154][155][156][157] However, EGFR activation can also disrupt cell-cell adhesion and promote cell migration. 124,158,159 Therefore, it is crucial to define the conditions, which render differential EGF responses.…”

“…94 Occludin is known to be highly phosphorylated on Threonine residues in the epithelium with intact tight junctions, 102,103,105 while it is dephosphorylated during the disruption of tight junctions by calcium depletion, 25 phorbol esters, 186 or acetaldehyde. 27,156,157 Occludin phosphorylation appears to be required for the assembly of tight junctions. 102,103,105 Evidence indicates that PP2A 59,60 and PKC 97,150,153 play roles in the regulation of Thrphosphorylation of occludin.…”