Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

Abstract

IntroductionEpidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs).Material and methodsHuman DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR.ResultsEGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect.ConclusionThese data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology.

Effects of Epidermal Growth Factor, Glial Cell Line-Derived Neurotrophic and Leukemia Inhibitory Factor on the Proliferation and Differentiation Potential of Adipose Tissue-Derived Mesenchymal Stem Cells

“…Conversely, it was reported that in lower doses of EGF (5 µg/mL) and bFGF (10 µg/mL), neural differentiation increased and differentiation into mesoderm lineage decreased (10). Also, EGF increased osteogenic differentiation and morphological change of dental pulp stem cells (36).…”

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

“…Especially, as factors resulting in stimulating loss of AP activity in the pSSCs, bFGF, and EGF included in pSSCCM can be considered, supported by the previous reports that bFGF and/or EGF induce differentiation of stem cells in the extracellular signal-free niche (Carpenter and Cohen, 1990; Schuldiner et al, 2000; Del Angel-Mosqueda et al, 2015). Accordingly, physico-chemical and physiological niche derived from GDNF-mESCCM will be able to be used as a basic microenvironment for investigating effects of extracellular signals on undifferentiation or differentiation of pSSCs.…”

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

“…Interestingly, our previous study also demonstrated that GP‐12 can significantly promote the proliferation of osteoblasts by interaction with epidermal growth factor receptor (EGFR) (Fig. S3), the signaling of which can affect bone formation by regulating endochondral ossification, and plays an essential role in the remodeling of the extracellular matrix . β ‐Catenin is involved in both the Wnt/ β ‐catenin and the EGFR pathways.…”