Epidermal growth factor enhances meiotic resumption of canine oocytes in the presence of BSA

Cui, Xiang‐Shun; Jin, Yong-Xun; Shen, Xiaopei; Lee, Jae Yeong; Lee, Hyo-Sang; Yin, Xi-Jun; Kong, Il‐Keun; Kim, Nam‐Hyung

doi:10.1016/j.theriogenology.2005.11.011

Cited by 22 publications

(15 citation statements)

References 27 publications

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“…These reports and our findings demonstrate that E 2 , but not DHT, induces morphologic and functional abnormalities of developing follicles, which results in the impairment of oocyte meiotic maturation. The treatment of oocytes with hCG/EGF induces meiotic resumption specially from GV to GVBD [30], [31]. In the present study, the high dose (10 −6 M) of E 2 blocked the progression from GVBD to MII in oocytes.…”

Section: Discussionsupporting

confidence: 53%

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

2014

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We have previously reported that androstenedione induces abnormalities of follicle development and oocyte maturation in the mouse ovary. In granulosa cells of the ovarian follicle, androstenedione is aromatized to 17β-estradiol (E2). To determine whether the androgen or estrogen acts directly on the follicle to induce the above-mentioned abnormalities, we compared the effects of a non-aromatizable androgen, 5α-dihydrotestosterone (DHT), with those of E2 on murine follicular development and oocyte maturation in a single follicle culture system. The high dose (10−6 M) of DHT prompted normal follicular development, and there was no effect on oocyte meiotic maturation after stimulation with human chorionic gonadotropin (hCG) and epidermal growth factor (EGF). In contrast, culture with the high dose (10−6 M) of E2 delayed follicular growth and also suppressed proliferation of granulosa cells and antrum formation. Furthermore, culture with E2 delayed or inhibited oocyte meiotic maturation, such as chromosome alignment on the metaphase plate and extrusion of the first polar body, after addition of hCG and EGF. In conclusion, these findings demonstrate that E2, but not DHT, induces abnormalities of follicular development, which leads to delay or inhibition of oocyte meiotic maturation.

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Section: Discussionsupporting

confidence: 53%

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

2014

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“…fetal calf, cow or bitch, in oestrus or not; Robertson et al 1992;Hewitt et al 1998;Rodrigues and Rodrigues 2003;Oh et al 2005;Lee et al 2007c); numerous growth factors such as IGF-1, epidermal growth factor (EGF) and human or porcine somatotropin (Rodrigues and Rodrigues 2003;Kim et al 2004;Bolamba et al 2006;Cui et al 2006;Rodrigues et al 2006;Hatoya et al 2009); various protein sources (Songsasen et al 2002;Rodrigues and Rodrigues 2003); hyaluronan (Rodrigues et al 2006); antioxidants (Kim et al 2004;Rota and Cabianca 2004;Hossein et al 2007;Lee et al 2007b); and cell cycle inhibitors (Hanna et al 2008). Nevertheless, Alhaider and Watson (2009) recently obtained high metaphase rates (up to 49%) with a combination of fibroblast growth factor (FGF), IGF, growth factor (GF) and transforming growth factor (TGF)-a.…”

Section: In Vitro Maturation Of Oocytesmentioning

confidence: 99%

The canine oocyte: uncommon features of in vivo and in vitro maturation

Chastant-Maillard

Lesegno

Chebrout

et al. 2011

Reprod. Fertil. Dev.

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The biology of the canine oocyte is unusual compared with that of other mammalian females. The present paper reviews both in vivo and in vitro specificities of canine oocytes. Final follicular growth in the bitch is characterised by an early appearance of LH binding sites in the granulosa, a high proportion of polyovular follicles and a preovulatory luteinisation, starting at the time of the LH surge. Through follicular fluid, preovulatory oocytes are thus exposed to high levels of progesterone, as high as 1000-fold plasma concentrations. The composition of the follicular fluid is affected by the size of the female. The more specific aspect of oocyte biology in the bitch is ovulation: oocytes are expelled immature, at the Prophase I stage. Ovulatory follicles are 6-8 mm in diameter, releasing oocytes from 110 µm, with dark cytoplasm. Resumption of meiosis occurs from 48 h postovulation, MII stages appearing 48-54 h after ovulation. The mechanisms controlling such a late meiotic resumption are still unknown. Granulosa cells seem to play a central role as in other mammalian species, but not with cAMP as the principal mediator. The importance of a transient reactivation of oocyte transcription a few hours before meiotic resumption is to be explored. These specific features may contribute to the low efficiency of IVM. Only 10-20% oocytes reach the metaphase stage and suffer from a poor cytoplasmic maturation. Moreover, in vitro culture of canine oocytes is associated with a high proportion of degeneration. To date, IVM of the oocytes is the main limiting factor for the development of assisted reproductive techniques in the canine. A better knowledge of the basic physiology of folliculogenesis and the molecular mechanisms controlling oocyte meiosis resumption in this species may allow us to overcome this obstacle.

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“…There are several reports indicating the role of media in vitro supplements on the meiotic maturation competence of canine oocytes (Otoi T et al 1999, Rodrigues and Rodrigues 2003, Cui et al 2006). However, the in vitro maturation procedures still bring low efficiency and only 15-20% of bitch oocytes reach the MII stage.…”

Section: Selected Aspects Of Canine Oocytes Maturation With Relation mentioning

confidence: 99%

The in vitro culture supplements and selected aspects of canine oocytes maturation

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Polish Journal of Veterinary Sciences

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The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved.In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.

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Epidermal growth factor enhances meiotic resumption of canine oocytes in the presence of BSA

Cited by 22 publications

References 27 publications

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

The canine oocyte: uncommon features of in vivo and in vitro maturation

The in vitro culture supplements and selected aspects of canine oocytes maturation

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