Epidermal Growth Factor Binding to Neonatal Mouse Skin Explants and Membrane Preparations—Effect of Triiodothyronine

Hoath, Steven B.; Lakshmanan, J.; Fisher, David A.

doi:10.1203/00006450-198503000-00004

Cited by 15 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although these transcripts have been detected in neonatal skin (Coffey et al, 1987) and adult skin (Elder et al, 1989) (Hoath et al, 1984(Hoath et al, , 1985Coffey et al, 1987;this paper) and the target EGFreceptors are present in human epidermis (Nanney et al, 1984;Green & Couchman, 1985) and other epithelia (Partridge et al, 1987). TGF-a protein has been detected by immunocytochemistry throughout all viable cell layers in neonatal foreskin (Coffey et al, 1987) and normal adult skin (Gottleib et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Production of TGF-α and TGF-β by cultured keratinocytes, skin and oral squamous cell carcinomas – potential autocrine regulation of normal and malignant epithelial cell proliferation

Partridge

Green²,

Langdon³

et al. 1989

Br J Cancer

View full text Add to dashboard Cite

Summary Transforming growth factors have a wide range of biological activities related to cell proliferation and differentiation. In general TGF-a promotes cell proliferation while TGF-P may stimulate or inhibit proliferation depending on the cell type and growth factor environment. Cultured human keratinocytes, skin and oral squamous cell carcinomas were analysed for the presence of transcripts and protein for the transforming growth factors a & P. Both growth factors were detected in cultured keratinocytes (which have receptors for and respond to both ligands), and in medium conditioned by these cells. Additionally transcripts for TGF-a were found preferentially in the basal, proliferative compartment of cultured keratinocytes. Similarly both growth factors were detected in oral squamous cell carcinomas and a highly significant inverse correlation was found between the levels of TGF-a and the epidermal growth factor receptor in these tumours. The data for TGF-a are consistent with the existence of an autocrine growth control loop influencing cell proliferation in both a normal cell type and malignant epithelial tissues, a process that in keratinocytes and responsive squamous cell carcinomas could be modulated by TGF-,B.

show abstract

Section: Discussionmentioning

confidence: 99%

Production of TGF-α and TGF-β by cultured keratinocytes, skin and oral squamous cell carcinomas – potential autocrine regulation of normal and malignant epithelial cell proliferation

Partridge

Green²,

Langdon³

et al. 1989

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…EGF binds to specific plasma membrane receptors that are found in many tissues and significant EGF binding to embryonal tissues has been reported (7,(10)(11)(12)(13)(14) An early indication of a close relation between thyroid hormones and EGF was found by Hamburgh et al (1 5) who showed that the delay of eyelid opening and tooth eruption observed in hypothyroid animals was reversed by either thyroxine or EGF administration. Further studies suggest that synthesis of both EGF and its receptor protein are influenced by thyroid hormones (3,4,12,(16)(17)(18)(19)(20). Recent studies of early postnatal T4 treatment (0-6 days) of newborn mice have shown a prolonged effect of the T4 on tissue and urine EGF levels and a prolonged effect on growth (21).…”

mentioning

confidence: 91%

“…In vivo, EGF elicits precocious tooth eruption and eyelid opening in rodents, accelerates lung maturation in lambs and reduces gastric acid secretion in man (4)(5)(6)(7)(8)(9). EGF binds to specific plasma membrane receptors that are found in many tissues and significant EGF binding to embryonal tissues has been reported (7,(10)(11)(12)(13)(14).…”

Section: The Affinity Of Egf Receptor Binding Initially Wasmentioning

confidence: 99%

“…Further studies suggest that synthesis of both EGF and its receptor protein are influenced by thyroid hormones (3,4,12,(16)(17)(18)(19)(20). Recent studies of early postnatal T4 treatment (0-6 days) of newborn mice have shown a prolonged effect of the T4 on tissue and urine EGF levels and a prolonged effect on growth (21).…”

Section: The Affinity Of Egf Receptor Binding Initially Wasmentioning

confidence: 99%

See 1 more Smart Citation

Neonatal Hyperthyroidism Alters Hepatic Epidermal Growth Factor Receptor Ontogeny in Mice

et al. 1988

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Epidermal growth factor (EGF) liver receptor ontogeny and somatic growth were studied in mice from day 7 to day 70 postnatally to assess long-term effects of short-term postnatal thyroxine treatment. The mice were given 0.4 pg thyroxinelg body weightlday for the 1st wk of life. EGF receptor binding in liver tissue was studied on days 7, 15, 20, 30, and 70 postnatally. Treated animals had accelerated eyelid opening and tooth eruption, and permanent growth retardation was obvious from the second week of life. Hepatic EGF receptor-binding capacity increased markedly in control mice with increasing age in contrast to a very slow increase in treated mice, making the difference statistically significant (P < 0.01) from day 30. The affinity of EGF receptor binding initially was similar in the two groups of animals (1.09 x lo9 M-' and 1.02 x lo9 M-') and increased by day 30 in controls (2.57 x lo9 M-I), an increase that was not observed in treated animals either at day 30 or 70. These results suggest a sensitive period of imprinting during the first 7 days postnatally, a period when thyroxine can exert a permanent effect on later growth and later hepatic EGF receptor number. (Pediatr Res 23: 557-560, 1988) Abbreviations EGF, epidermal growth factor T4, thyroxine TSH, thyroid-stimulating hormone Thyroid hormones are necessary for postnatal growth and development in rodents as in humans although the mechanism of the thyroid hormone effect is not entirely clear (1). Recent studies suggest that thyroid hormone interaction with specific growth factors may be important for normal postnatal growth and development (2). An interaction with the growth hormonesomatomedin axis has been described (2). Several other growth factors have been studied, but to date the interaction between thyroid hormones and EGF is the best characterized (3,4). EGF is a polypeptide (mouse EGF mol. wt. 6045) first described in extracts from mouse submandibular glands (5-7). In vivo, EGF elicits precocious tooth eruption and eyelid opening in rodents, accelerates lung maturation in lambs and reduces gastric acid secretion in man (4-9). EGF binds to specific plasma membrane receptors that are found in many tissues and significant EGF binding to embryonal tissues has been reported (7,(10)(11)(12)(13)(14) An early indication of a close relation between thyroid hormones and EGF was found by Hamburgh et al. (1 5) who showed that the delay of eyelid opening and tooth eruption observed in hypothyroid animals was reversed by either thyroxine or EGF administration. Further studies suggest that synthesis of both EGF and its receptor protein are influenced by thyroid hormones (3,4,12,(16)(17)(18)(19)(20). Recent studies of early postnatal T4 treatment (0-6 days) of newborn mice have shown a prolonged effect of the T4 on tissue and urine EGF levels and a prolonged effect on growth (21). This effect was not observed in mice treated only during the 2nd wk of life (2 1). The present study was undertaken to relate the disturbances in somatic growth to the postn...

show abstract

“…EGF production in mouse SMG is thyroxine and testosterone responsive and urine EGF production and kidney EGF concentrations are thyroxine responsive in the mouse (10)(11)(12). EGF concentrations in several other tissues including liver, skin, and eye also are thyroxine responsive (13,14) as is EGF receptor binding in skin and liver (15,16). Finally, EGF has been shown to evoke hormone secretion from cultured anterior pituitary cells (17)(18)(19), and a recent paper reports that EGF augments ACTH release in vivo in adult sheep (20).…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor acts as a corticotropin-releasing factor in chronically catheterized fetal lambs.

Polk¹,

Ervin²,

Padbury³

et al. 1987

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

show abstract

Epidermal Growth Factor Binding to Neonatal Mouse Skin Explants and Membrane Preparations—Effect of Triiodothyronine

Cited by 15 publications

References 11 publications

Production of TGF-α and TGF-β by cultured keratinocytes, skin and oral squamous cell carcinomas – potential autocrine regulation of normal and malignant epithelial cell proliferation

Production of TGF-α and TGF-β by cultured keratinocytes, skin and oral squamous cell carcinomas – potential autocrine regulation of normal and malignant epithelial cell proliferation

Neonatal Hyperthyroidism Alters Hepatic Epidermal Growth Factor Receptor Ontogeny in Mice

Epidermal growth factor acts as a corticotropin-releasing factor in chronically catheterized fetal lambs.

Contact Info

Product

Resources

About