Epidermal growth factor binding and receptor distribution in the mouse reproductive tract during development

Bossert, Nancy L.; Nelson, Kay M.; Ross, Kimberly A.; Takahashi, Tsuneo; McLachlan, John A.

doi:10.1016/0012-1606(90)90152-9

Cited by 31 publications

(17 citation statements)

References 33 publications

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“…Because estrogen stimulates uterine epithelial cell proliferation (McCormack & Glasser 1980, Quarmby & Korach 1984 and expression of EGF, IGF-I, heparin-binding EGF-like growth factor, transforming growth factor (TGF)-and EGF-R in the reproductive tracts of mice and rats, these growth factors may mediate the estrogen-induced proliferative and differentiative responses in rodent reproductive tracts (Murphy et al 1987, DiAugustine et al 1988, Nelson et al 1991, Beck & Garner 1992, Ignar-Trowbridge et al 1992, Kapur et al 1992, Das et al 1994, Sahlin et al 1994, Zhang et al 1994, Falck & Forsberg 1996, Hom et al 1998. In the fetal reproductive tract, expression of EGF-R in mice (Bossert et al 1990), and EGF, TGFand IGF-I in rats (Koike & Noumura 1993, Kanno et al 1994, Gu et al 1999) was reported. Kanno et al (1994) reported that immunoreactivity of EGF was not-detected, moderate, slight and moderate during GD 15, GD 16-17, GD 18-20 and GD 21 respectively, in the female rat Müllerian duct.…”

Section: Discussionmentioning

confidence: 99%

Effect of diethylstilbestrol on cell proliferation and expression of epidermal growth factor in the developing female rat reproductive tract

Okada¹,

Satō²,

Ohta³

et al. 2001

Journal of Endocrinology

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To evaluate mechanisms of cell proliferation in the fetal female rat reproductive tract, diethylstilbestrol (DES) effects on cell division and estrogen receptor (ER), epidermal growth factor (EGF) and EGF receptor (EGF-R) expressions were determined from gestational day (GD) 15·5 to 21·5. Reproductive tracts were evaluated within three regions along the Müllerian duct axis; these were proximal, middle and caudal, which differentiate into oviduct, uterus and upper vagina respectively. In fetuses from non-treated dams, epithelial and mesenchymal proliferation, as evaluated by 5-bromo-2 -deoxyuridine incorporation, was decreased with development in all regions of the Müllerian duct. EGF levels were determined by immunohistochemistry. Müllerian epithelial EGF immunoreactivity was intense in the proximal and middle regions on GDs 15·5 and 17·5. EGF staining remained intense only in the proximal epithelia by GD 19·5 and was weak in the caudal epithelium, but substantially reduced throughout epithelia in all regions by GD 21·5. Thus, decreased cell proliferation correlated with decreased EGF expression in the developing Müllerian duct. DES (100 µg/kg body weight) was injected from GD 15 to 19 and female fetuses were collected on GD 19·5. DES increased Müllerian duct cell proliferation in the proximal epithelium and mesenchyme but decreased it in the caudal epithelium compared with oil-treated controls. No proliferative DES effect was observed in any cell type in the middle region. Müllerian duct EGF immunoreactivity was suppressed by DES compared with oil. Competitive RT-PCR indicated DES also decreased mRNAs for EGF, ER 1 and ER 2, but not ER and EGF-R. These results indicate EGF may be an important regulatory factor of Müllerian duct cell proliferation, and that DES may alter cell proliferation by disrupting normal EGF, ER 1 and ER 2 expression in the developing female rat reproductive tract.

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Section: Discussionmentioning

confidence: 99%

Effect of diethylstilbestrol on cell proliferation and expression of epidermal growth factor in the developing female rat reproductive tract

Okada¹,

Satō²,

Ohta³

et al. 2001

Journal of Endocrinology

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“…EGF levels fall on day 2, but TGF-␣ expression continues till day 3 and then increases in the stroma [Tamada et al, 1991]. EGFr is expressed throughout the preimplantation period (days 1-5) in the myometrium, stroma, and epithelium [Bossert et al, 1990;Das et al, 1994a], although it is a matter of controversy whether a functional signal-transducing EGFr is expressed in the luminal epithelium [Tong et al, 1996]. Consistent with their role in mediating the proliferative effects of E 2 , EGF, TGF-␣, and the EGFr are all upregulated (within 6 hours) following the administration of E 2 to ovariectomized mice [HuetHudson et al, 1990;Paria et al, 1993Paria et al, , 1994.…”

Section: Cell Proliferation and Differentiation In The Uterus: Their mentioning

confidence: 98%

Preimplantation development of the mammalian embryo and its regulation by growth factors

Stewart¹,

Cullinan

1997

Dev. Genet.

130

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“…It is now recognized that the EGF receptor binds a number of ligands, including EGF, transforming growth factor a (TGFa), amphiregulin (Ar), and heparin-binding EGF (HB-EGF), all of which activate the receptor [1,2]. The receptor, EGF, and TGFa have been found in the uterus of rodents [3][4][5][6][7][8]10] and humans [11][12][13][14] and also in the fallopian tubes of humans [14]. To our knowledge, there is no information about the expression of Ar or HB-EGF in the female reproductive tract of any species.…”

Section: Introductionmentioning

confidence: 99%

Expression of the Genes for the Epidermal Growth Factor Receptor and its Ligands in Porcine Oviduct and Endometrium1

Kennedy¹,

Brown²,

Vaughan³

1994

Biology of Reproduction

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We have used the reverse transcription-polymerase chain reaction (RT-PCR) to determine whether transcripts for the epidermal growth factor (EGF) receptor and its four known ligands--EGF, transforming growth factor alpha (TGF alpha), amphiregulin (Ar), and heparin-binding EGF (HB-EGF)--are expressed in porcine oviduct and endometrium. We were able to detect mRNA for the EGF receptor, EGF, TGF alpha, and Ar in both the oviduct and endometrium, whereas HB-EGF mRNA was not detectable in either tissue. Through use of an antiserum raised against recombinant pig EGF, expression of EGF was found to be localized to the columnar epithelial cells of the oviduct and to the glandular epithelial cells of the endometrium. The possible physiological roles of the EGF family in the reproductive tract are discussed.

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Epidermal growth factor binding and receptor distribution in the mouse reproductive tract during development

Cited by 31 publications

References 33 publications

Effect of diethylstilbestrol on cell proliferation and expression of epidermal growth factor in the developing female rat reproductive tract

Effect of diethylstilbestrol on cell proliferation and expression of epidermal growth factor in the developing female rat reproductive tract

Preimplantation development of the mammalian embryo and its regulation by growth factors

Expression of the Genes for the Epidermal Growth Factor Receptor and its Ligands in Porcine Oviduct and Endometrium1

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