Epidermal growth factor activates EGFR/AMPK signalling to up-regulate the expression of SGLT1 and GLUT2 to promote intestinal glucose absorption in lipopolysaccharide challenged IPEC-J2 cells and piglets

Tang, Xiangwei; Xiong, Kangning

doi:10.1080/1828051x.2022.2073832

Cited by 7 publications

(12 citation statements)

References 41 publications

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“…The results of this study showed that dietary EGF supplementation significantly increased VH and V/C, which indicated that EGF could repair the intestinal morphology of piglets with IUGR. Similarly, our previous study showed that EGF treatment could repair intestinal morphology in LPS-stimulated piglets [ 23 ]. Pearson’s correlation analysis showed that VH and V/C were positively correlated with the ADFI, ADG, and FBW of piglets, which means that a good intestinal morphology is essential for animal growth.…”

Section: Discussionmentioning

confidence: 68%

“…The serum TP level can reflect the status of protein synthesis and metabolism [ 33 ]. Glucose is essential for maintaining the energy and health status of animals and regulating the absorption and transport capacity of various nutrients [ 18 , 23 ]. Immunoglobulins (IgA, IgG, and IgM) play important roles in immunity, which are considered important indicators reflecting the health status of animals [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that IUGR can cause intestinal villus shedding, crypt hyperplasia, and intestinal mucosal atrophy and damage intestinal mucosal barrier function, thereby resulting in the decline of intestinal absorption function [ 4 , 9 ]. EGF, as an intestinal trophic factor, plays important roles in intestinal development and intestinal repair [ 15 , 16 , 23 ]. The results of this study showed that dietary EGF supplementation significantly increased VH and V/C, which indicated that EGF could repair the intestinal morphology of piglets with IUGR.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose is an important energy source in the body and is absorbed mainly in the small intestine [ 36 ]. It has been proved that the glucose absorption in the intestinal tract is achieved through either the paracellular or transcellular route, in which the Na + /glucose cotransporter (SGLT1)-mediated transcellular route is the major route for the glucose transport [ 4 , 23 , 37 , 38 ]. GLUT2, a facilitated Na + -independent monosaccharide transporter, also contributes to glucose absorption; it can be recruited transiently to the apical membrane and assist in glucose absorption in response to a sugar-rich meal [ 39 ] or intestinal repair process [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, there are little data on EGF as a nutritional additive to alleviate intestinal injury in IUGR offspring. Our previous study showed that EGF treatment can promote glucose absorption of damaged intestinal or intestinal epithelial cells of piglets [ 18 , 23 ], which means that glucose plays an important role in intestinal repair. However, whether EGF can promote intestinal repair of IUGR piglets by increasing intestinal glucose absorption has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Dietary Epidermal Growth Factor Supplementation Alleviates Intestinal Injury in Piglets with Intrauterine Growth Retardation via Reducing Oxidative Stress and Enhancing Intestinal Glucose Transport and Barrier Function

Tang

Xiong

2022

Animals

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EGF plays an important role in the intestinal repair and nutrients transport of animals. However, the effect of EGF on the intestinal health of piglets with IUGR has not been reported. Thus, the present study was performed to investigate the effects of EGF on the intestinal morphology, glucose absorption, antioxidant capacity, and barrier function of piglets with IUGR. A total of 6 NBW piglets and 12 IUGR piglets were randomly divided into three treatments: NC group (NBW piglets fed with basal diet, n = 6), IC group (IUGR piglets fed with basal diet, n = 6), and IE group (IUGR piglets fed with basal diet supplemented with 2 mg/kg EGF, n = 6). Growth performance, serum biochemical profile, jejunum histomorphology, jejunum glucose absorption and antioxidant capacity, and jejunal barrier function were measured. The results showed that EGF supplementation significantly increased the final body weight (FBW), average daily gain (ADG), and average daily feed intake (ADFI) of piglets with IUGR; EGF supplementation significantly increased the total protein (TP), glucose (GLU), and immunoglobulin G (IgG) levels compared with the IUGR piglets in the IC group; EGF administration effectively exhibited an increased jejunum villus height (VH) and the villus-height-to-crypt-depth ratio (V/C) of IUGR piglets compared with the IC group; EGF supplementation significantly increased sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) activity, intestinal alkaline phosphatase (AKP) activity, glucose transporter sodium/glucose cotransporter 1 (SGLT1), glucose transporter 2 (GLUT2), and AMP-activated protein kinase α1 (AMPK-α1) mRNA expressions in the jejunum of IUGR piglets compared with the IC group; EGF supplementation exhibited increased superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) levels, tended to increase glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and tended to decrease the malondialdehyde (MDA) level in the jejunum of IUGR piglets compared with the IC group; EGF supplementation significantly increased ZO-1, Claudin-1, Occludin, and MUC2 mRNA expressions and improved secreted immunoglobulin A (sIgA) secretion in the jejunum of IUGR piglets compared with the IC group and tended to decrease the interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α) levels in the jejunum of IUGR piglets compared with the IC group. Pearson’s correlation analysis further showed that EGF can promote intestinal development and nutrient absorption by promoting intestinal barrier function, thus improving the growth performance of IUGR piglets.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary Epidermal Growth Factor Supplementation Alleviates Intestinal Injury in Piglets with Intrauterine Growth Retardation via Reducing Oxidative Stress and Enhancing Intestinal Glucose Transport and Barrier Function

Tang

Xiong

2022

Animals

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Red rice bran aqueous extract ameliorate diabetic status by inhibiting intestinal glucose transport in high fat diet/STZ-induced diabetic rats

Ontawong,

Pengnet,

Thim-Uam

et al. 2024

Journal of Traditional and Complementary Medicine

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Lipopolysaccharide promotes apoptosis and oxidative injury of porcine small intestinal epithelial cells by down-regulating the expression of glutamine transporter ASCT2

Tang

Xiong

Jia³

et al. 2023

Journal of Animal Science

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The present study aimed to investigate the effects of lipopolysaccharide (LPS) stimulation on oxidative damage, apoptosis, and glutamine (Gln) transporter Alanine-Serine-Cysteine transporter 2 (ASCT2) expression in porcine small intestinal epithelial cells (IPEC-J2), and preliminarily elucidated the relationship between ASCT2 expression level and oxidative damage and apoptosis of IPEC-J2 cells. IPEC-J2 cells were treated without (control group, CON, n=6) or with 1 μg/mL LPS (LPS group, LPS, n=6). Cell viability, lactate dehydrogenase (LDH) content, malonaldehyde (MDA), antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC)], apoptosis of IPEC-J2 cells, the expression of Caspase3, the expression of ASCT2 mRNA and ASCT2 protein was detected. The results showed that LPS stimulation of IPEC-J2 cells significantly reduced the cell viability, and antioxidant enzymes activity (SOD, CAT and GSH-Px), and significantly increased LDH and MDA release. Flow cytometry results showed that LPS stimulation significantly increased the late apoptosis rate and the total apoptosis rate of IPEC-J2 cells. The immunofluorescence results showed that the fluorescence intensity of LPS stimulated IPEC-J2 cells was significantly enhanced. LPS stimulation significantly decreased the mRNA and protein expression of ASCT2 in IPEC-J2 cells. The correlation analysis showed that ASCT2 expression was negatively correlated with apoptosis, and positively correlated with the antioxidant capacity of IPEC-J2 cells. According to the results of this study, it can be preliminarily concluded that LPS promotes the apoptosis and oxidative injury of IPEC-J2 cells by down-regulating the expression of ASCT2.

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Epidermal growth factor activates EGFR/AMPK signalling to up-regulate the expression of SGLT1 and GLUT2 to promote intestinal glucose absorption in lipopolysaccharide challenged IPEC-J2 cells and piglets

Cited by 7 publications

References 41 publications

Dietary Epidermal Growth Factor Supplementation Alleviates Intestinal Injury in Piglets with Intrauterine Growth Retardation via Reducing Oxidative Stress and Enhancing Intestinal Glucose Transport and Barrier Function

Dietary Epidermal Growth Factor Supplementation Alleviates Intestinal Injury in Piglets with Intrauterine Growth Retardation via Reducing Oxidative Stress and Enhancing Intestinal Glucose Transport and Barrier Function

Red rice bran aqueous extract ameliorate diabetic status by inhibiting intestinal glucose transport in high fat diet/STZ-induced diabetic rats

Lipopolysaccharide promotes apoptosis and oxidative injury of porcine small intestinal epithelial cells by down-regulating the expression of glutamine transporter ASCT2

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