Epidermal fatty-acid-binding protein: a new circulating biomarker associated with cardio-metabolic risk factors and carotid atherosclerosis

Yeung, Dennis C.Y.; Wang, Yudong; Xu, Aimin; Cheung, Stephen C.W.; Wat, Nelson M.S.; Fong, Daniel Tik-Pui; Fong, Carol Ho Yi; Chau, MT; Sham, Pak C.; Lam, Karen S.L.

doi:10.1093/eurheartj/ehn295

Cited by 40 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the expression of FABP5 is only about one-hundredth of that of FABP4 in adipose tissue [51]. Furthermore, circulating FABP5 level is detected at levels of about one tenth or less of FABP4 concentrations [25, 36, 37]. Levels of FABP4 and FABP5 in the local area around ADSC should be much higher than those in circulating blood, which are about 1 nM (15 ng/ml) for FABP4 and 0.1 nM (1.5 ng/ml) for FABP5 [25], and we therefore used recombinant FABP4 and FABP5 at the dose of 1 μM in in vitro experiments in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, secretion of FABP5 remains to be elucidated. However, circulating FABP5, similar to FABP4, has been reported to be detected at levels of about one tenth or less of FABP4 concentrations, and FABP5 level has been shown to be associated with components of metabolic syndrome, though the correlation is not as strong as that of FABP4 [25, 36, 37]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome and Metabolome Analyses in Exogenous FABP4- and FABP5-Treated Adipose-Derived Stem Cells

Yamamoto¹,

Furuhashi²,

Sugaya³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Adipose-derived stem cells (ADSC), which exist near adipocytes in adipose tissue, have been used as a potential tool of regenerative medicine. Lipid chaperones, fatty acid-binding protein 4 (FABP4) and 5 (FABP5), are abundantly expressed in adipocytes. FABP4 has recently been shown to be secreted from adipocytes during lipolysis in a non-classical pathway and may act as an adipokine. Here, we investigated the role of exogenous FABP4 and FABP5 in transcriptional and metabolic regulation in ADSC. FABP4 and FABP5 were little expressed in ADSC. However, both FABP4 and FABP5 were significantly induced after adipocyte differentiation of ADSC and were secreted from the differentiated adipocytes. Analysis of microarray data, including gene ontology enrichment analysis and cascade analysis of the protein-protein interaction network using a transcription factor binding site search, demonstrated that treatment of ADSC with FABP4 or FABP5 affected several kinds of genes related to inflammatory and metabolic responses and the process of cell differentiation. Notably, myogenic factors, including myocyte enhancer factors, myogenic differentiation 1 and myogenin, were modulated by treatment of ADSC with FABP4, indicating that exogenous FABP4 treatment is partially associated with myogenesis in ADSC. Metabolome analysis showed that treatment of ADSC with FABP4 and with FABP5 similarly, but differently in extent, promoted hydrolysis and/or uptake of lipids, consequentially together with enhancement of β oxidation, inhibition of downstream of the glycolysis pathway, accumulation of amino acids, reduction of nucleic acid components and increase in the ratio of reduced and oxidized nicotinamide adenine dinucleotide phosphates (NADPH/NADP+), an indicator of reducing power, and the ratio of adenosine triphosphate and adenosine monophosphate (ATP/AMP), an indicator of the energy state, in ADSC. In conclusion, secreted FABP4 and FABP5 from adipocytes as adipokines differentially affect transcriptional and metabolic regulation in ADSC near adipocytes. The adiposity condition in the host of regenerative medicine may affect characteristics of ADSC by exposure of the balance of FABP4 and FABP5.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses in Exogenous FABP4- and FABP5-Treated Adipose-Derived Stem Cells

Yamamoto¹,

Furuhashi²,

Sugaya³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…A member of the iLBP subfamily IV, FABP5 binds a wide array of ligands in a 1:1 ratio, including fatty acids and fatty acid metabolites spanning 10 -22 carbons in length with various saturation states, as well as the vitamin A metabolite all-trans-retinoic acid and numerous synthetic drugs and probes (10,(23)(24)(25). It has also been found to be involved in a range of pathologies, including the metabolic syndrome (26,27), atherosclerosis (28), cancer (29 -33), and potentially certain neurodegenerative diseases (34).…”

mentioning

confidence: 99%

Structural Basis for Ligand Regulation of the Fatty Acid-binding Protein 5, Peroxisome Proliferator-activated Receptor β/δ (FABP5-PPARβ/δ) Signaling Pathway

Armstrong

Goswami

Griffin

et al. 2014

Journal of Biological Chemistry

112

133

View full text Add to dashboard Cite

Background: Intracellular lipid-binding proteins stimulate lipid-induced gene expression. Results: Fatty acid-binding protein 5 (FABP5) uses a molecular switch that controls nuclear import when complexed with activating fatty acids. Conclusion: FABP5 is tuned to selectively stimulate peroxisome proliferation-activated receptor ␤/␦ transactivation in response to specific fatty acids based on their structural features. Significance: FABPs provide a second level of regulatory control of nuclear receptor-mediated lipid signaling.

show abstract

“…Altogether, previous data suggest that A-FABP has some hormonal functions on other tissues after release from adipose tissue. As for other FABPs, a few studies suggest that some FABPs such as L-FABP and E-FABP may have roles in systemic metabolism and cardiovascular system by showing the association between their serum concentrations, and metabolic and cardiovascular regulation markers (27283435). However, it has not been investigated, to our knowledge, that whether the secreted form of L-FABP is biologically active.…”

Section: Discussionmentioning

confidence: 99%

Cytokine-like Activity of Liver Type Fatty Acid Binding Protein (L-FABP) Inducing Inflammatory Cytokine Interleukin-6

et al. 2016

View full text Add to dashboard Cite

It has been reported that fatty acid binding proteins (FABPs) do not act only as intracellular mediators of lipid responses but also have extracellular functions. This study aimed to investigate whether extracellular liver type (L)-FABP has a biological activity and to determined serum L-FABP levels in patients with end-stage renal disease (ESRD). We isolated L-FABP complementary deoxyribonucleic acid (cDNA) from the Huh7 human hepatocarcinoma cell line and expressed the recombinant L-FABP protein in Escherichia coli. A549 lung carcinoma and THP-1 monocytic cells were stimulated with the human recombinant L-FABP. Human whole blood cells were also treated with the human recombinant L-FABP or interleukin (IL)-1α. IL-6 levels were measured in cell culture supernatants using IL-6 enzyme-linked immunosorbent assay (ELISA). Human recombinant L-FABP induced IL-6 in a dose-dependent manner in A549, THP-1 cells, and whole blood cells. The blood samples of healthy volunteers and patients with ESRD were taken after an overnight fast. The serum levels of L-FABP in healthy volunteers and ESRD patients were quantified with L-FABP ELISA. The values of L-FABP in patients with ESRD were significantly lower than those in the control group. Our results demonstrated the biological activity of L-FABP in human cells suggesting L-FABP can be a mediator of inflammation.

show abstract

Epidermal fatty-acid-binding protein: a new circulating biomarker associated with cardio-metabolic risk factors and carotid atherosclerosis

Abstract: E-FABP is a new circulating biomarker associated with increased cardio-metabolic risk. It may contribute to the development of the MetS and carotid atherosclerosis in humans, independent of the effect of A-FABP.

Cited by 40 publications

References 29 publications

Transcriptome and Metabolome Analyses in Exogenous FABP4- and FABP5-Treated Adipose-Derived Stem Cells

Transcriptome and Metabolome Analyses in Exogenous FABP4- and FABP5-Treated Adipose-Derived Stem Cells

Structural Basis for Ligand Regulation of the Fatty Acid-binding Protein 5, Peroxisome Proliferator-activated Receptor β/δ (FABP5-PPARβ/δ) Signaling Pathway

Cytokine-like Activity of Liver Type Fatty Acid Binding Protein (L-FABP) Inducing Inflammatory Cytokine Interleukin-6

Contact Info

Product

Resources

About