Epidermal-Dermal Separation with Proteolytic Enzymes*

Einbinder, Julia M.; Walzer, Richard; Mandl, Ines

doi:10.1038/jid.1966.75

Cited by 43 publications

(9 citation statements)

References 12 publications

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“…sweating). This latter observation agrees with that of Einbinder, Walzer, and Mandl (1966) although no acantholysis was observed in these experiments as was reported in Einbinder's work. The lower part of the ORS has separated from the CTS (Fig.…”

Section: (I) Depilation Load 61· 2 (10 Kr)supporting

confidence: 81%

“…Other workers (Einbinder, Walzer, and Mandl 1966) have observed that the first effect of treatment of skin with proteolytic enzymes is vacuolation and perinuclear halo formation in the cells of the basal layers, followed by dermal-epidermal separation. With Pancrozyme CIA no changes were observed at this stage in the Figs.…”

Section: (B) Structural Changes In the Follicle Following Applicationmentioning

confidence: 99%

“…The changes described above are those for the most advanced state of degradation at each depilatory level, but especially in the early stages many follicles had not reached the stage of degradation described. It is of interest in this context that Einbinder, Walzer, and Mandl (1966) find that skins must be incubated in enzyme solution for consistent dermal and epidermal changes to occur, and that topical application of enzymes gives inconsistent results. This variability in the trypsin system is undoubtedly a contributing factor to the unsuitability of trypsin as a depilatory enzyme.…”

Section: (Iv) Depilation Load 1· 3 (59 Hr)mentioning

confidence: 99%

“…12, Puckered remains of the ORS (fully nucleated) at the top of the follicle after fibre removal. cells of the basal layer, but with the bacterial method of wool loosening ("sweating") (Yates 1968a) dermal-epidermal separation was preceded by vacuolation of the cells as described by Einbinder, Walzer, and Mandl (1966).…”

Section: (B) Structural Changes In the Follicle Following Applicationmentioning

confidence: 99%

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Studies in Depilation VII. Structural Changes in the Wool Follicle During Depilation With Acid and Enzyme Systems

Yates¹

1968

Aust. Jnl. Of Bio. Sci.

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SummaryStructural changes in the wool follicle during depilation with acetic acid, crystalline trypsin, and a commercial enzyme preparation, Pancrozyme C1A, are described. A number of the morphological changes produced by acetic acid and Pancrozyme C1A are similar. Both cause early separation of the epidermal and outer root sheath (ORS) structures from the underlying tissues, and the resistance to fibre withdrawal at this stage is due largely to the physical obstruction of the ORS material. In both cases the separated ORS assumes a tightly packed cylindrical form around the fibre and is gradually digested away until there is no longer any resistance to fibre removal. In both cases separation of the fibre ultimately occurs in the lower part of the prekeratinous zone just above the bulb.Histological evidence is provided which accounts for the unsatisfactory nature of crystalline trypsin as a depilating agent.A comparative analysis of the main structural changes in the follicle with the depilating systems at present available is given, and the requirements for an ideal depilating system are stated.

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Section: (I) Depilation Load 61· 2 (10 Kr)supporting

confidence: 81%

Section: (B) Structural Changes In the Follicle Following Applicationmentioning

confidence: 99%

Section: (Iv) Depilation Load 1· 3 (59 Hr)mentioning

confidence: 99%

Section: (B) Structural Changes In the Follicle Following Applicationmentioning

confidence: 99%

See 2 more Smart Citations

Studies in Depilation VII. Structural Changes in the Wool Follicle During Depilation With Acid and Enzyme Systems

Yates¹

1968

Aust. Jnl. Of Bio. Sci.

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“…Responses of the basal cell to exogenous toxins and proteases such as papain (4), collagenase (5) pronase (6), and the reported ultrastructural pathology of other skin lesions (7) are all strikingly similar to the HD-induced pathology of the skin basal cell and, now, to that of the human lymphocyte in vitro. In addition, the temporal features of HD-induced lesion, to include the effect on the epidermal-dermal junction, are also similar.…”

Section: Discussicnmentioning

confidence: 85%