Epidermal CCL27 Expression Is Regulated during Skin Development and Keratinocyte Differentiation

Mildner, Michael; Prior, Marion; Gschwandtner, Maria; Schuster, Christopher; Tschachler, Erwin; Elbe‐Bürger, Adelheid

doi:10.1038/jid.2013.394

Cited by 12 publications

(13 citation statements)

References 14 publications

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“…Whether the enhanced CCL27 signal we identified in the inner foreskin is responsible for an influx or induction of CCR10-expressing HIV target cells warrants further investigation. Our experiments of adding exogenous CCL27 to inner foreskin tissue cultures did reveal that this chemokine induced the movement of tissue-resident CD3+CD4+ T cells to the epithelium of the inner foreskin and may be compatible with in vitro monolayer and organotypic skin cultures showing that CCL27, expressed by epidermal keratinocytes 33 , enhanced the influx of CCR10+CD4+ T cells into inflamed skin. Our CCL27 data also appear to be reminiscent of studies that have investigated wound healing 33,34 , where it has been shown that upon burn-wound injury, CCL27 produced by keratinocytes promotes tissue repair.…”

Section: Discussionsupporting

confidence: 65%

Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

et al. 2020

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We compared outer and inner foreskin tissue from adolescent males undergoing medical male circumcision to better understand signals that increase HIV target cell availability in the foreskin. We measured chemokine gene expression and the impact of sexually transmitted infections (STIs) on the density and location of T and Langerhans cells. Chemokine C-C ligand 27 (CCL27) was expressed 6.94-fold higher in the inner foreskin when compared to the outer foreskin. We show that the density of CD4+CCR5+ cells/mm2 was higher in the epithelium of the inner foreskin, regardless of STI status, in parallel with higher CCL27 gene expression. In the presence of STIs, there were higher numbers of CD4+CCR5+ cells/mm2 cells in the sub-stratum of the outer and inner foreskin with concurrently higher number of CD207+ Langerhans cells (LC) in both tissues, with the latter cells being closer to the keratin surface of the outer FS in the presence of an STI. When we tested the ability of exogenous CCL27 to induce T cell migration in foreskin tissue, CD4+ T cells were able to relocate to the inner foreskin epithelium in response. We provide novel insight into the impact CCL27 and STIs on immune and HIV-1 target cell changes in the foreskin.

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Section: Discussionsupporting

confidence: 65%

Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

et al. 2020

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“…The levels of IL‐6, IL‐8, and IL‐10 were measured but were all below detection levels in adult and fetal skin (lower limit detection levels were 0.88 pg/mg, 0.63 pg/mg, and 1.63 pg/mg, respectively). Further, the main T‐cell chemoattractants during skin homeostasis are CCL17 and CCL27 . Fetal skin contained significant lower levels of both CCL17 and CCL27 (Figure B, C).…”

Section: Resultsmentioning

confidence: 95%

Healthy human second‐trimester fetal skin is deficient in leukocytes and associated homing chemokines

Walraven

Talhout

Beelen

et al. 2016

Wound Repair Regeneration

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The lack of immune cells in mid-gestational fetal skin is often mentioned as a key factor underlying scarless healing. However, the scarless healing ability is conserved until long after the immune system in the fetus is fully developed. Therefore, we studied human second-trimester fetal skin and compared the numbers of immune cells and chemokine levels from fetal skin with adult skin. By using immunohistochemistry, we show that healthy fetal skin contains significant lower numbers of CD68(+) -macrophages, Tryptase(+) -mast cells, Langerin(+) -Langerhans cells, CD1a(+) -dendritic cells, and CD3(+) -T cells compared to adult skin. Staining with an early lineage leukocyte marker, i.e., CD45, verified that the number of CD45(+) -immune cells was indeed significantly lower in fetal skin but that sufficient numbers of immune cells were present in the fetal lymph node. No differences in the vascular network were observed between fetal and adult skin. Moreover, significant lower levels of lymphocyte chemokines CCL17, CCL21, and CCL27 were observed in fetal skin. However, levels of inflammatory interleukins such as IL-6, IL-8, and IL-10 were undetectable and levels of CCL2 were similar in healthy fetal and adult skin. In conclusion, this study shows that second-trimester fetal skin contains low levels of immune cells and leukocyte chemokines compared to adult skin. This immune cell deficiency includes CD45(+) leukocytes, despite the abundant presence of these cells in the lymph node. The immune deficiency in healthy second-trimester fetal skin may result in reduced inflammation during wound healing, and could underlie the scarless healing capacities of the fetal skin.

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“…Besides, IL17 is supposed to downregulate CCL27 expression [22], and also further cytokines overexpressed in psoriasis, such as INF-g and TNF-a, have a pivotal role in downregulating CCL27 expression at later stages of psoriatic plaques [23]. Interestingly, only differentiated keratinocytes -which are known to be rather amiss in psoriasis -have been shown to strongly release CCL27 [24].…”

mentioning

confidence: 99%

NOS2 and CCL27: clinical implications for psoriasis and eczema diagnosis and management

Garzorz

Eyerich

2014

Expert Review of Clinical Immunology

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Epidermal CCL27 Expression Is Regulated during Skin Development and Keratinocyte Differentiation

Cited by 12 publications

References 14 publications

Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

Healthy human second‐trimester fetal skin is deficient in leukocytes and associated homing chemokines

NOS2 and CCL27: clinical implications for psoriasis and eczema diagnosis and management

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